1 research outputs found
Multimodal Mass Spectrometry Imaging of <i>N</i>‑Glycans and Proteins from the Same Tissue Section
On-tissue
digestion matrix-assisted laser desorption/ionization mass spectrometry
imaging (MALDI-MSI) can be used to record spatially correlated molecular
information from formalin-fixed, paraffin-embedded (FFPE) tissue sections.
In this work, we present the <i>in situ</i> multimodal analysis
of <i>N</i>-linked glycans and proteins from the same FFPE
tissue section. The robustness and applicability of the method are
demonstrated for several tumors, including epithelial and mesenchymal
tumor types. Major analytical aspects, such as lateral diffusion of
the analyte molecules and differences in measurement sensitivity due
to the additional sample preparation methods, have been investigated
for both <i>N</i>-glycans and proteolytic peptides. By combining
the MSI approach with extract analysis, we were also able to assess
which mass spectral peaks generated by MALDI-MSI could be assigned
to unique <i>N</i>-glycan and peptide identities