EXPOSURE TO GOAT BILE FOR 28-DAYS CAUSES HEPATOCYTE INJURY: A HISTOPATHOLOGICAL STUDY

Highlights: 1. Bile consumption, especially goat bile, is believed to have therapeutic effects even though it contains harmful ingredients that can cause toxic effects on the liver 2. The administration of goat bile for 28 days had a toxic effect on the liver of the mice based on histopathological findings Abstract: Background: Bile consumption by Indonesians is believed to have therapeutic effects, especially goat bile. Goat bile is thought to contain harmful ingredients that can cause toxic effects on the liver. However, the 28-days oral toxicity study of goat bile has not been performed. Objective: To analyze the hepatotoxic effect of subchronic administration of goat bile on the liver of mice (Mus musculus). Material and Method: This was an experimental research with a post-test-only control group design. The samples used were 32 Balb/C mice (Mus musculus), which were grouped into 4 groups. The samples were administered with goat bile orally (3.2, 6.4, or 12.8 mL/kg/day) for 28 days. The liver was taken for histopathological examination and the hepatocytes injury score was performed. The scoring results were analyzed using Kruskal-Wallis, Mann-Whitney, and Spearman correlation tests (p<0.05). Result: Goat bile administration was associated with hepatocyte injury (p= 0.004). Groups with goat bile administration of 6.4 and 12.8 mL/kg/day had significant differences with the control group (p= .015 and .029 respectively) and the 3.2 mL/kg/day administered group (p= 0.006 and 0.009 respectively). Moreover, the increased administration of goat bile had a positive correlation with the level of hepatocyte injury (p= 0.004 and r_s= 0.504) Conclusion: Goat bile administration for 28 days had a significant toxic effect on the liver of mice at a dose of 6.4 mL/kg/day

UJI TOKSISITAS SUBKRONIS ORAL EMPEDU KAMBING TERNAK (Capra aegagrus hircus) TERHADAP HATI MENCIT (Mus musculus)

Konsumsi empedu oleh orang Indonesia dipercaya dapat memberikan efek terapetik, khusus empedu kambing. Empedu kambing diperkirakan mengandung zat berbahaya yang dapat mengakibatkan efek toksik pada hati. Walaupun demikian, uji toksisitas subkronis oral empedu kambing belum pernah dilakukan. Penelitian ini adalah penelitian eksperimental dengan desain post-test-only control group. Sampel yang digunakan adalah 32 ekor mencit (Mus musculus) strain BalbC, yang dibagi menjadi 4 kelompok perlakuan. Empedu kambing diberikan secara oral dengan dosis 3,2; 6,4; dan 12,8 mL/KgBB/Hari selama 28 hari. Hati mencit diambil untuk pemeriksaan histopatologi, lalu skoring kerusakan hepatosit juga dilakukan. Hasil skoring dianalisis secara statistik dengan uji Kruskal – Wallis, Mann – Whitney, dan uji korelasi Spearman (p<0.05). Hasil: Pemberian empedu kambing berhubungan dengan kerusakan hepatosit (p= 0,004). Kelompok perlakuan dengan dosis pemberian 6,4 dan 12,8 mL/KgBB/Hari menunjukkan perbedaan efek toksik yang signifikan dengan kelompok kontrol negatif (p= 0,015 dan 0,029) dan kelompok dosis 3,2 mL/KgBB/Hari (p= 0,006 dan 0,009). Selain itu, peningkatan dosis pemberian empedu kambing memiliki korelasi yang positif dengan tingkat kerusakan hepatosit (p= .004) Kesimpulan: Pemberian empedu kambing ternak menimbulkan efek toksis pada hati mencit dimulai pada dosis 6,4 mL/KgBB/Hari. Kata Kunci: Empedu kambing, asam empedu, uji toksisitas subkronis, hepatotoksisita