2 research outputs found
Induction of the expression of defence genes in Carica papaya fruit by methyl jasmonate and low temperature treatments
Abstract The defence mechanisms that are activated by methyl jasmonate
(MJ) in fruits are not well understood. In this work, we studied the
expression of defence genes in papaya fruit that are induced by the
exposure to MJ and/or low temperatures. The papaya fruits
\u2018Maradol\u2019 were randomly divided into two groups: one group
was the untreated control and the other was treated with 10-4 M of MJ.
Half of the fruits from each of the two groups were stored after
treatment for 5 days at 5\ubaC and 2 days at 20\ubaC. We studied
the expression levels of the pdf1.1 and pdf1.2 genes by amplification
from expression libraries created from the pulp and skin tissues of the
papaya fruit. As a reference, the mRNA level of the 18s ribosomal gene
was used. In the skin tissue, the expression levels of the pdf1.1 and
pdf1.2 genes were higher immediately after MJ treatment compared to the
control. Furthermore, the expression of pdf1.2 remained high after MJ
treatment and subsequent storage compared to the control. It was
therefore concluded that the activation of the pdf1.1 and pdf1.2 genes
forms part of the molecular defence mechanism in fruits that is
activated by exposure to MJ. To our knowledge, this is the first study
that analyzes the gene expression in papaya fruit that is induced by
the exogenous application of methyl jasmonate and cold treatment
Total RNA quality of lyophilized and cryopreserved dormant grapevine buds
Background: Plant tissues must be preserved in their collection state,
especially for genome-wide expression profile studies. Lyophilization
is a feasible, affordable tool when fresh tissues cannot be shipped at
ultralow temperatures from their origin to the place of analysis. In
this study, the total RNA quality of dormant grapevine buds ( Vitis
vinifera L. cv. \u2018Flame Seedless\u2019) of freeze-dried
samples stored at room temperature conditions was evaluated and
compared to that of cryopreserved (-80\ub0C) grapevine buds. Results:
Good yield and quality of RNA were obtained from freeze-dried dormant
buds stored at room temperature for 0, 3 and 6 weeks after they were
lyophilized. Further experiments confirmed that the extracted total RNA
could be used for actin and \u3b2-tubulin PCR gene amplification.
Conclusion: High-quality RNA that is useful for downstream applications
was obtained from freeze-dried dormant grapevine bud tissue, similarly
to the RNA obtained from cryopreserved dormant grapevine buds