Bactericidal effect of silver nanoparticles against propagation of Clavibacter michiganensis infection in Lycopersicon esculentum Mill

This study explored the use of silver nanoparticle as a bactericidal against the propagation of Clavibacter michiganensis onto tomatoes (Lycopersicon esculentum Mill). In Mexico, tomato production covers about 73% of the total vegetable production but it is affected by outbreak of bacteria canker caused by Clavibacter michiganensis subspecies michiganensis (Cmm). Silver ions possess inhibitor properties, bactericides and high specter antimicrobials. In this study, 6 groups of culture were prepared using 6 different petri dishes where silver nanoparticles of varying concentrations (120, 84, 48, 24, 12 and 0 μg) were added. Furthermore, each group was observed for 20 min, 1, 2, 12 and 24 h. The optimum concentration is 84 μg, which shows an average of 2 Cmm colonies after 20 min. Further increase to 120 μg shows no significant change. However, the average colonies was observed for 48 μg after 1, 2, 12, and 24 h. The obtained results indicate that silver nanoparticles are a promising inhibitor, bactericide and high a specter antimicrobial for treatment or prevention of Cmm

Antagonistic trait of Staphylococcus succinus strain AAS2 against uropathogens and assessment of its in vitro probiotic characteristics

The desideratum aim of the present context was to isolate a promising antagonist probiotic bacterium from fermented food item as biocontrol agent against uropathogens. Among diversified isolates evaluated for antagonistic trait, Staphylococcus succinus strain AAS2 was found to be an auspicious candidate against urinary tract infection (UTI) causing bacterial pathogens, being the most active against Staphylococcus aureus with substantial activity of 352.5 ± 5.4 AU/mL. Further, the in vitro probiotic attributes of strain AAS2 were assessed using systematic methodology. The isolate exhibited tolerance to acidic condition (up to pH 3.0) and simulated gastric juice (at pH 3.0) with fairly high survival logarithmic cell counts of 5.3 ± 0.15 and 5.23 ± 0.02 log cfu/mL, respectively. Additionally, strain AAS2 showed capability to resist 0.5% w/v bile salt too. It also revealed significant values of auto-aggregation (32.5 ± 1.3–56.5 ± 1.4%) and cell surface hydrophobicity (38.35 ± 1.4%) properties. The isolate showed resistivity towards phenol (6.8 ± 0.08 log cfu/mL) and lysozyme (58.6 ± 1.6%). Further, the susceptibility trait of strain AAS2 to conventional antibiotics made this isolate a promising probiotic bacterium. Most importantly, the isolate depicted DPPH (2,2-Diphenyl-1-picrylhydrazyl) and hydroxyl radical scavenging activities in a concentration dependent manner, thereby exhibiting its propitious antioxidative properties. In a nutshell, the outcomes of this investigation divulge the plausible use of S. succinus strain AAS2 as biocontrol agent against uropathogens, and recommended further applications in pharmaceutics due to its pronounced probiotic traits

PREVALENCIA DE COCCIDIA EN BECERRAS HOLSTEIN EN LA ETAPA DE DESARROLLO

Tanto la producción como el consumo de leche han incrementado, es por eso que la salud de los becerros es fundamental. La importancia de la dieta y el cuidado de los becerros determinan las pérdidas o ganancias que un productor de leche puede tener. De la muestra bajo supervisión, el 62 % resultaron positivos con coccidia, el 81 % de la muestra tuvo niveles de inmunoglobulina menores a 7.0 mg/ml confirmando el alto porcentaje de becerros enfermos o propensos a enfermarse

CULTIVO DE EMBRIONES A TRAVÉS DE UN MEDIO MODIFICADO PARA AUMENTAR LA PREÑEZ EN GANADO BOVINO

Las condiciones de cultivo in vitro mejoran la calidad del embrión durante el desarrollo temprano y tardío, y modificaciones en las condiciones de cultivo pueden aumentar la expresión génica, el metabolismo celular o el estado de la impresión genómica. La investigación se enfocó en el metabolismo del embrión que fue medido en un medio modificado describiendo las principales vías por las cuales se implementa el Adenosin Trifosfato. Los medios usados para la capacitación espermática fueron HTF QUEEN, HTF HEPES, SPERM WASH MEDIUM, también se utilizó ATP. El espermatozoide fue incubado para la fertilización in vitro junto con los medios modificados para luego transferir los embriones a las vacas receptoras. El embrión tuvo un mejor desarrollo en presencia del ATP que con los medios estándares usados, preñándose 2 de las 5 vacas