Adivinhas e desenvolvimento da inferência na educação pré-escolar

O presente estudo pretendia desenvolver a competência inferencial em crianças a frequentar a Educação Pré-Escolar mediante o recurso a adivinhas. Com a realização deste projeto, pretendíamos obter resposta para a seguinte questão de investigação: Que efeito terá a exploração de adivinhas no desenvolvimento da capacidade inferencial em crianças a frequentar a Educação Pré-Escolar? O nosso objetivo de investigação era: desenvolver competências em inferência em crianças a frequentar a Educação Pré-Escolar, tirando proveito do lado lúdico da adivinha e do uso que faz do pensamento inferencial. Assim, concebemos e implementamos uma intervenção didática, incluindo cinco sessões, que incidiu sobre a realização de inferências pragmáticas (de objeto, agente, lugar e tempo) a partir de adivinhas apresentadas às crianças e de adivinhas que elas próprias produziram. Recorrendo a uma metodologia de investigação de índole qualitativa, baseada no estudo de caso, recolhemos dados relativos ao desempenho das crianças em atividades envolvendo a exploração de adivinhas e a sua criação. A análise de conteúdo dos dados recolhidos revelou que estas crianças tinham efetivamente desenvolvido a sua competência inferencial, um elemento-chave da compreensão oral e escrita. No entanto, apresentavam algumas dificuldades nas situações que envolviam inferências de agente e tempoThis study aimed to develop the ability to make inferences in children attending a kindergarten (3-5 year old) using riddles. The project was supposed to help find answers for a research question: In what way the exploration of riddles will affect the ability to make inferences in children attending kindergarten? Our research objective was the following one: To develop the ability to make inferences in children attending kindergarten through the exploration of riddles and taking profit of their amusing nature and their use of inference. With this purpose, we conceived and applied a didactic intervention, including five sessions, focused on the elaboration of pragmatic inferences (object, agent, time and place) through the exploration and the production of riddles. Using a qualitative research methodology, based on case study, we collected data concerning the children’s ability to make inferences by involving them in activities related with finding the answers for riddles and creating their own riddles. The content analysis of the data revealed that these children had really developed their ability to make several kinds of inferences, a key element of oral and written comprehension. Nevertheless, they were less able when the riddles demanded inferences concerning agent and timeMestrado em Educação Pré-Escolar e Ensino no 1º Ciclo do Ensino Básic

Preparation of starch-based scaffolds for tissue engineering by supercritical immersion precipitation

The aim of this study was to evaluate the possibility of preparing starch-based porous matrixes using supercritical fluid technology. Supercritical immersion precipitation technique was used to prepare scaffolds of a polymeric blend of starch and poly(l-lactic acid) for tissue engineering purposes.Immersion precipitation experiments were carried out at different operational conditions and highly porous and interconnected scaffolds were obtained. Two organic solvents, dichloromethane and chloroform were tested, and from the results obtained chloroform was the more favourable for the process. The effect of polymer solution concentration (5 up to 20 wt%), temperature (35 up to 55 ◦C) and pressure (100 up to 200 bar) in the SPLA (50:50 wt%) membrane morphology, porosity and interconnectivity was evaluated. All the conditions tested were in the region of total miscibility between the organic solvent and carbon dioxide. Additionally, a blend with a different starch-poly(l-lactic acid) ratio (30:70 wt%) was tested. Bicontinuous structures were formed indicating that the L–L demixing process that governs the phase inversion is the spinodal decomposition.Ana Rita C. Duarte is grateful for financial support from Fundacao para a Ciencia a Tecnologia through the grant SFRH/BPD/34994/2007

Dexamethasone-loaded scaffolds prepared by supercritical assisted phase inversion

The aim of this study was to evaluate the possibility of preparing dexamethasone-loaded starch-based porous matrices in a one-step process. Supercritical phase inversion technique was used to prepare composite scaffolds of dexamethasone and a polymeric blend of starch and poly(L-lactic acid) (SPLA) for tissue engineering purposes. Dexamethasone is used in osteogenic media to direct the differentiation of stem cells towards the osteogenic lineage. Samples with different drug concentrations (5–15 wt.% polymer) were prepared at 200 bar and 55 C. The presence of dexamethasone did not affect the porosity or interconnectivity of the polymeric matrices. Water uptake and degradation studies were also performed on SPLA scaffolds. We conclude that SPLA matrices prepared by supercritical phase inversion have a swelling degree of nearly 90% and the material presents a weight loss of 25% after 21 days in solution. Furthermore, in vitro drug release studies were carried out and the results show that a sustained release of dexamethasone was achieved over 21 days. The fitting of the power law to the experimental data demonstrated that drug release is governed by an anomalous transport, i.e., both the drug diffusion and the swelling of the matrix influence the release of dexamethasone out of the scaffold. The kinetic constant was also determined. This study reports the feasibility of using supercritical fluid technology to process in one step a porous matrix loaded with a pharmaceutical agent for tissue engineering purposes.Ana Rita C. Duarte is grateful for financial support from Fundatyao para a Ciencia a Tecnologia through the Grant SFRH/BPD/34994/2007

Polymer processing using supercritical fluid based technologies for drug delivery and tissue engineering applications

From the use of botanical plants in early human civilizations through synthetic chemistry and biotechnology, drug research has always passionate scientists creating exciting challenges to a large number of researchers from different fields, thus, promoting a collaborative effort between polymer scientists, pharmacologists, engineers, chemists and medical researchers. Worldwide, there is an increasing concern on health care that creates a major opportunity for development of new pharmaceutical formulations. Ageing populations worried about the quality of life in the older years are actively seeking for new, more effective and patient compliant drug delivery devices. This has been the driving force for the continuous growth of the research made on delivery devices, which has become a powerful technique in health care. It has been recognized for long that simple pills or injections may not be the suitable methods of administration of a certain active compound. These medications present several problems and/or limitations, like poor drug bioavailability and systemic toxicity, derived essentially from pharmacokinetic and other carrier limitations and low solubility of the drugs in water. Therefore and to overcome these drawbacks, clinicians recommend frequent drug dosing, at high concentrations, in order to overcome poor drug bioavailability but causing a potential risk of systemic toxicity. Polymer science has open new strategies for drug delivery systems. This Chapter overviews of possible strategies involving polymer modification and processing for controlled drug delivery and drug delivery in tissue engineering.European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement number REGPOT-CT2012-316331-POLARIS and from the project “Novel smart and biomimetic materials for innovative regenerative medicine approaches” RL1 -ABMR-NORTE-01-0124-FEDER-000016 cofinanced by North Portugal Regional Operational Programme (ON.2 –O Novo Norte), under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF)

The role of organic solvent on the preparation of chitosan scaffolds by supercritical assisted phase inversion

The aim of this study was to evaluate the possibility of preparing chitosan porous matrixes using supercritical fluid technology. Supercritical immersion precipitation technique was used to prepare scaffolds of a natural biocompatible polymer, chitosan for tissue engineering purposes. The physicochemical and biological properties of chitosan make it an excellent material for the preparation of drug delivery systems and for the development of new biomedical applications in many fields from skin to bone or cartilage. Supercritical assisted phase inversion experiments were carried out and the effect of different organic solvents on the morphology of the scaffolds was assessed. Chitosan scaffold morphology, porosity and pore size were evaluated by SEM and micro-CT. A thermodynamic analysis of the process was carried out and insights on the solubility parameter and Flory–Huggins interaction parameters are given. The preparation of a highly porous and interconnected structure of a natural material, chitosan, using a clean and environmentally friendly technology constitutes a new processing technology for the preparation of scaffolds for tissue engineering using these materials.Ana Rita C. Duarte is grateful for financial support from Fundacao para a Ciencia e Tecnologia (FCT) through the grant SFRH/BPD/34994/2007. The support through the FCT project PTDC/QUI/68804/2006 is also acknowledged

Preparation of chitosan scaffolds for tissue engineering using supercritical fluid technology

The aim of this study was to evaluate the possibility of preparing chitosan porous matrixes using supercritical fluid technology. Supercritical immersion precipitation technique was used to prepare scaffolds of a natural biocompatible polymer, chitosan, for tissue engineering purposes. The physicochemical and biological properties of chitosan make it an excellent material for the preparation of drug delivery systems and for the development of new biomedical applications in many fields from skin to bone or cartilage. Immersion precipitation experiments were carried out at different operational conditions in order to optimize the processing method. The effect of different organic solvents on the morphology of the scaffolds was assessed. Additionally, different parameters that influence the process were tested and the effect of the processing variables such as polymer concentration, temperature and pressure in the chitosan scaffold morphology, porosity and interconnectivity was evaluated by micro computed tomography. The preparation of a highly porous and interconnected structure of a natural material, chitosan, using a clean and environmentally friendly technology constitutes a new processing technology for the preparation of scaffolds for tissue engineering using these materials

Thermosensitive polymeric matrices for three-dimensional cell culture strategies

A completely new strategy for cell culture focusing on the design of three-dimensional (3D) smart surfaces by supercritical fluid technology has been developed. This approach might overcome the limitations on cell expansion and proliferation of currently existing techniques. An alternative technology, based on supercritical carbon dioxide, was used to polymerize poly(N-isopropylacrylamide) (PNIPAAm) and to foam poly(d,l-lactic acid) (PD,LLA), creating a thermosensitive 3D structure which has proven to have potential as a substrate for cell growth and expansion. We demonstrated that the thermosensitive matrices promoted cell detachment, thus PD,LLA scaffolds have the potential to be used as substrates for cell growth and expansion avoiding enzymatic and mechanical methods of cell harvesting. The harvested cells were replated to evaluate their viability, which was not compromised. A major advantage of this technology is the fact that the prepared materials can be recovered and reused. Therefore, the same substrate can be recycled and reused for different batches. An indirect impact of the technology developed is related to the field of biotechnology, as this novel technology for cell expansion can be applied to any adherent cell cultures.FCT project PTDC/QUI/68804/2006; grant SFRH/BPD/34994/200

Novel 3D scaffolds of chitosan-PLLA blends for tissue engineering applications : preparation and characterization

This work addresses the preparation of 3D porous scaffolds of blends of chitosan and poly(l-lactic acid), CHT and PLLA, using supercritical fluid technology. Supercritical assisted phase-inversion was used to prepare scaffolds for tissue engineering purposes. The physicochemical and biological properties of chitosan make it an excellent material for the preparation of drug delivery systems and for the development of new biomedical applications in many fields from skin to bone or cartilage regeneration. On the other hand, PLLA is a synthetic biodegradable polymer widely used for biomedical applications. Supercritical assisted phase-inversion experiments were carried out in samples with different polymer ratios and different polymer solution concentrations. The effect of CHT:PLLA ratio and polymer concentration and on the morphology and topography of the scaffolds was assessed by SEM and Micro-CT. Infra-red spectroscopic imaging analysis of the scaffolds allowed a better understanding on the distribution of the two polymers within the matrix. This work demonstrates that supercritical fluid technology constitutes a new processing technology, clean and environmentally friendly for the preparation of scaffolds for tissue engineering using these materials