Acoustic Response of a Vibrating Elongated Cylinder in a Hydrodynamic Turbulent Flow

The present paper contains the results of the numerical analysis of the interaction between a Newtonian incompressible turbulent flow and a linear elastic slender body, together with the influence of the fluid-structure interaction (FSI) on the noise generation and propagation. The purpose is to evaluate the differences in term of acoustic pressure between the case where the solid body is rigid (infinite stiffness) and the case where it is elastic (finite stiffness). A partitioned and implicit algorithm with the arbitrary Lagrangian-Eulerian method (ALE) is used for the interaction between the fluid and solid. For the evaluation of the turbulent fluid motion, we use a large eddy simulation (LES) with the Smagorinsky subgrid scale model. The equation for the solid is solved through the Lagrangian description of the momentum equation and the second Piola-Kirchoff stress tensor. In addition, the acoustic analogy of Lighthill is used to characterize the acoustic source (the slender body) by directly using the fluid dynamic fields. In particular, we use the Ffowcs Williams and Hawkings (FW-H) equation for the evaluation of the acoustic pressure in the fluid medium. As a first numerical experiment, we analyze a square cylinder immersed in a turbulent flow characterized by two different values of stiffness: one infinite (rigid case) and one finite (elastic case). In the latter case, the body stiffness and mean flow velocity are such that they induce the lock-in phenomenon. Finally, we evaluate the differences in terms of acoustic pressure between the two different cases

Establishing Human Lacrimal Gland Cultures with Secretory Function

PURPOSE: Dry eye syndrome is a multifactorial chronic disabling disease mainly caused by the functional disruptions in the lacrimal gland. The treatment involves palliation like ocular surface lubrication and rehydration. Cell therapy involving replacement of the gland is a promising alternative for providing long-term relief to patients. This study aimed to establish functionally competent lacrimal gland cultures in-vitro and explore the presence of stem cells in the native gland and the established in-vitro cultures. METHODS: Fresh human lacrimal gland from patients undergoing exenteration was harvested for cultures after IRB approval. The freshly isolated cells were evaluated by flow cytometry for expression of stem cell markers ABCG2, high ALDH1 levels and c-kit. Cultures were established on Matrigel, collagen and HAM and the cultured cells evaluated for the presence of stem cell markers and differentiating markers of epithelial (E-cadherin, EpCAM), mesenchymal (Vimentin, CD90) and myofibroblastic (α-SMA, S-100) origin by flow cytometry and immunocytochemistry. The conditioned media was tested for secretory proteins (scIgA, lactoferrin, lysozyme) post carbachol (100 µM) stimulation by ELISA. RESULTS: Native human lacrimal gland expressed ABCG2 (mean±SEM: 3.1±0.61%), high ALDH1 (3.8±1.26%) and c-kit (6.7±2.0%). Lacrimal gland cultures formed a monolayer, in order of preference on Matrigel, collagen and HAM within 15-20 days, containing a heterogeneous population of stem-like and differentiated cells. The epithelial cells formed 'spherules' with duct like connections, suggestive of ductal origin. The levels of scIgA (47.43 to 61.56 ng/ml), lysozyme (24.36 to 144.74 ng/ml) and lactoferrin (32.45 to 40.31 ng/ml) in the conditioned media were significantly higher than the negative controls (p<0.05 for all comparisons). CONCLUSION: The study reports the novel finding of establishing functionally competent human lacrimal gland cultures in-vitro. It also provides preliminary data on the presence of stem cells and duct-like cells in the fresh and in-vitro cultured human lacrimal gland. These significant findings could pave way for cell therapy in future

Reti ecologiche in agricoltura. Struttura, funzionamento e modellizzazione del paesaggio vegetale e dell’agroecosistema

This work presents principal results of a research over ecological networks in hilly and low-mountain agro-ecosystems in the Marche Region (Central Italy). Different studies have been carried out to collect data coming from multiple research fields. In particular a phytosociological analysis was realized in order to study and describe all natural and semi-natural vegetable units inside the landscape. A faunistic study was also carried out in order to assess the impact of agricultural practices on Carabid Beatles and Micromammals populations, used as bio-indicators of biodiversity and habitat fragmentation. These studies were used to create a modelling system for landscape quality evaluation, as a method to assess evolution dynamics of different life-forms inside ecosystems modified by human activities. This model represents a tool for different users (agronomists, biologists, architects, environmental scientists etc.), available for both analytical and management purposes. It can be used to describe and locate homogeneous areas with precise productive potentiality, and to assess policies and local measures effectiveness