Evaluation of surface properties of erythrocyte membranes in liver diseases

Background: The physicochemical properties of Red Blood Cell membranes (RBC) are altered in liver diseases. Langmuir monolayers offer an excellent model system to study biological membrane surface properties. The aim of this study was to evaluate surface properties of erythrocyte membranes in liver diseases.  Methods: Sixty-one patients with various liver diseases and fifteen controls were enrolled. Surface properties of RBC membrane were evaluated using Langmuir monolayers. Surface pressure area isotherms were recorded at body temperature using RBC membrane lipid extract. Student’s t-test and Analysis of variance tests were performed.Results: Mean maximum surface pressure and hysteresis area were significantly higher in cirrhotic and non-cirrhotic liver disease groups compared to controls. Within cirrhotics, mean maximum surface pressure and lift off area was significantly lower in the Child C group as compared to the Child A, B and A-B groups. The mean hysteresis area was significantly lower in the Child C group as compared to the Child B and A-B groups.Conclusion: The results of our study confirmed high rigidity of RBC membrane in mild and moderate liver cirrhosis and high fluidity in severe liver cirrhosis. This study may pave the way to the development of a surface activity based biophysical tool for therapeutic implication in liver diseases.

Nanotechnology platforms in Parkinson’s Disease

Parkinson’s disease (PD) remains a serious concern due to its effects on the quality of life of patients and its socioeconomic burden to society. Present day management of PD has limitations in both diagnosis and treatment. Nanotechnology may provide smart solutions to this problem. The present review highlights the recent advancements in the development of nanotechnology platforms for PD. The review focuses on the use of such platforms in diagnostics, treatments, deep brain stimulation, neurosurgery and other modalities of management and the role of nanotechnology in each of these fields. The review also sheds light on the translation of technologies from labs to clinics and the essential advantages as well as concerns that accompany the translation

Interfacial properties as predictors of radioresistance in cervical cancer

The prediction of radioresistance of tumours, early in the course of radiotherapy, may help clinicians in deciding the optimal treatment strategy for each case. This study was carried out to investigate an in vitro technique to predict radiosensitivity, after a single radiation dose of 2 Gy in cervical cancer. Langmuir films of tissue homogenates of biopsy samples from 20 cervical cancer patients treated with radiotherapy alone and 15 normal controls were evaluated. The tensiometric profiles before and after giving 2 Gy of radiation, were compared with that of controls and were correlated with the clinical radioresponsiveness evaluated on completion of the radiotherapy course of 70–78 Gy over a period of 50–55 days. The tensiometric profiles measured after a single dose of radiation can be used to fingerprint the clinical radioresponsiveness of the cervical cancer tissues. The hysteresis of the monolayers of completely radioresponsive post-radiotherapy tissue homogenates was 5.8 times greater than that of partially radioresponsive post-radiotherapy tissue homogenates and was statistically significant using Mann–Whitney test (p < 0.05). From our results, the following tensiometric criteria for prediction of radioresistance emerge. After first dose of radiation, if the minimum surface tension of tissue homogenate is greater than 50 mN/m and hysteresis area is less than 20 μJ those tissues will be in the partially radioresponsive and for completely radioresponsive tissue homogenates, the minimum surface tension will be less than 47 mN/m and the hysteresis area will be greater than 33 μJ. The cholesterol and phospholipid content of radioresponsive cervical cancerous tissues after radiotherapy was found to be 1.2 and 2.2 times lower than that of the untreated tissues and due to lower lipid content organic phase surface activity of radioresponsive cancerous tissues after radiotherapy was less than that of the untreated tissue organic phase. The radiation induced tensiometric profile changes of radioresponsive cervical cancerous tissues can be correlated to the radiation induced lipid profile changes. This technique, due to its simplicity and high precision, can serve as a predictive tool for radioresponsiveness and is easily translatable to the clinical setting. Randomized large sample trials are necessary to validate this technique further and help in the translation from bench to clinics.© Elsevie

Cellular interactions of lauric acid and dextran-coated magnetite nanoparticles

In vitro cytocompatibility and cellular interactions of lauric acid and dextran-coated magnetite nanoparticles were evaluated with two different cell lines (mouse fibroblast and human cervical carcinoma). Lauric acid-coated magnetite nanoparticles were less cytocompatible than dextran-coated magnetite nanoparticles and cellular uptake of lauric acid-coated magnetic nanoparticles was more than that of dextran-coated magnetite nanoparticles. Lesser cytocompatibility and higher uptake of lauric acid-coated magnetite nanoparticles as compared to dextran-coated magnetic nanoparticles may be due to different cellular interactions by coating material. Thus, coating plays an important role in modulation of biocompatibility and cellular interaction of magnetic nanoparticles.© Elsevie