29 research outputs found
Inhibition of proliferation in (A and C) MCF-7 WT or (B and D) MCF-7 ADR cultures with lipoxygenase inhibitors; MK59I, MK886, NDGA for A and B; curcumin, ketoconazole, and AA88I for C and D
<p><b>Copyright information:</b></p><p>Taken from "Control of the growth of human breast cancer cells in culture by manipulation of arachidonate metabolism"</p><p>BMC Cancer 2007;7():138-138.</p><p>Published online 25 Jul 2007</p><p>PMCID:PMC1940258.</p><p></p> Cells were plated in IMEM culture fluid containing 8% FBS, 0.5% penicillin and streptomycin sulfate,0.5% kanamycin sulfate, and 1% MEM Vitamin Solution. Inhibitors were added after 24 hours and the cells were incubated in a humidified chamber of 5% CQfor an additional 48 hours. Thymidine was incorporated 16 hours prior to harvest and the cells were harvested using Packard Unifilter System. Values are expressed as % of the control. Data points represent the mean ± SD of three experiments with samples run in replicates of six
Human bone marrow stroma colonies treated with inhibitors for the first week and fresh media for the second week
<p><b>Copyright information:</b></p><p>Taken from "Control of the growth of human breast cancer cells in culture by manipulation of arachidonate metabolism"</p><p>http://www.biomedcentral.com/1471-2407/7/138</p><p>BMC Cancer 2007;7():138-138.</p><p>Published online 25 Jul 2007</p><p>PMCID:PMC1940258.</p><p></p> (A) The number of colonies, (B) average area of the colonies, (C) and the percentage of coverage of the colonies are shown. The corresponding concentration of inhibitors were added to the cells which were plated in IMDM-based Long Term Culture Media containing 25% horse serum and incubated in a humidified chamber containing 5% CQ. Data points represent the mean ± SD of three experiments
Induction of apoptosis in MCF-7 WT cells with (B) Curcumin, (C) NDGA and (D) MK 886
<p><b>Copyright information:</b></p><p>Taken from "Control of the growth of human breast cancer cells in culture by manipulation of arachidonate metabolism"</p><p>http://www.biomedcentral.com/1471-2407/7/138</p><p>BMC Cancer 2007;7():138-138.</p><p>Published online 25 Jul 2007</p><p>PMCID:PMC1940258.</p><p></p> MCF-7 WT cells were plated in Labtek 8 chamber glass slides for 24 hours and then incubated with the inhibitors for 48 hours. Cells were fixed using paraformaldehyde and stained with HOECHST 33258 dye for 30 minutes. Cells with bright, fragmented, condensed nuclei were identified as apoptotic cells
Cells were treated with each inhibitor and the lipoxygenase activity was determined in treated and control cells
<p><b>Copyright information:</b></p><p>Taken from "Control of the growth of human breast cancer cells in culture by manipulation of arachidonate metabolism"</p><p>http://www.biomedcentral.com/1471-2407/7/138</p><p>BMC Cancer 2007;7():138-138.</p><p>Published online 25 Jul 2007</p><p>PMCID:PMC1940258.</p><p></p> Data points represent the mean ± SD of three experiments with samples run in triplicate
Human bone marrow stroma colonies treated with inhibitors continuously for 2 weeks
<p><b>Copyright information:</b></p><p>Taken from "Control of the growth of human breast cancer cells in culture by manipulation of arachidonate metabolism"</p><p>http://www.biomedcentral.com/1471-2407/7/138</p><p>BMC Cancer 2007;7():138-138.</p><p>Published online 25 Jul 2007</p><p>PMCID:PMC1940258.</p><p></p> (A) The number of colonies, (B) average area of the colonies, (C) and the percentage of coverage of the colonies are shown. Cells were treated in the same manner as described in figure 5. Data points represent the mean ± SD of three independent experiments
Percentage of isolates tested for recommended antibiotics for shigellosis.
Percentage of isolates tested for recommended antibiotics for shigellosis.</p
Percentage of ciprofloxacin resistance in shigellosis, 2001–2020.
Percentage of ciprofloxacin resistance in shigellosis, 2001–2020.</p
Percentage of azithromycin resistance in shigellosis, 2001–2020.
Percentage of azithromycin resistance in shigellosis, 2001–2020.</p
Percentage of different Shigella species in under 5 children in the urban site and rural site in last 20 years.
Percentage of different Shigella species in under 5 children in the urban site and rural site in last 20 years.</p
Percentage of antibiotic resistance and association with year among under 5 children with shigellosis from 2001–2020 in rural site (Matlab hospital).
Percentage of antibiotic resistance and association with year among under 5 children with shigellosis from 2001–2020 in rural site (Matlab hospital).</p