94 research outputs found
Evaluation and molecular characterization of human adenovirus in drinking water supplies: viral integrity and viability assays
BACKGROUND: Human adenoviruses (HAdVs) are the second-leading cause of childhood gastroenteritis worldwide. This virus is commonly found in environmental waters and is very resistant to water disinfection and environmental stressors, especially UV light inactivation. Molecular techniques, such as PCR-based methods (Polymerase Chain Reaction), are commonly used to detect and identify viral contamination in water, although PCR alone does not allow the discrimination between infectious and non-infectious viral particles. A combination of cell culture and PCR has allowed detection of infectious viruses that grow slowly or fail to produce cytopathic effects (CPE) in cell culture. This study aimed to assess the integrity and viability of human adenovirus (HAdV) in environmental water and evaluate circulating strains by molecular characterization in three sites of the water supply in Florianópolis, Santa Catarina Island, Brazil: Peri Lagoon water, spring source water, and water from the public water supply system. METHODS: Water samples were collected, concentrated and HAdV quantified by real-time PCR. Viral integrity was evaluated by enzymatic assay (DNase I) and infectivity by plaque assay (PA) and integrated cell culture using transcribed mRNA (ICC-RT-qPCR). Samples containing particles of infectious HAdV were selected for sequencing and molecular characterization. RESULTS: The analyzed sites contained 83, 66 and 58% undamaged HAdV particles (defined as those in which the genetic material is protected by the viral capsid) at Peri Lagoon, spring source water and public supply system water, respectively. Of these, 66% of the particles (by PA) and 75% (by ICC-RT-qPCR) HAdV were shown to be infectious, due to being undamaged in Peri Lagoon, 33% (by PA) and 58% (by ICC-RT-qPCR) in spring source water and 8% (by PA) and 25% (by ICC-RT-qPCR) in the public water supply system. ICC-RT-qPCR, a very sensitive and rapid technique, was able to detect as low as 1 × 10(2) HAdV genome copies per milliliter of infectious viral particles in the environmental water samples. The molecular characterization studies indicated that HAdV-2 was the prevalent serotype. CONCLUSIONS: These results indicate a lack of proper public health measures. We suggest that HAdV can be efficiently used as a marker of environmental and drinking water contamination and ICC-RT-qPCR demonstrated greater sensitivity and speed of detection of infectious viral particles compared to PA
Agroecologia e justiça ambiental: um diálogo possível
O movimento por justiça ambiental surgiu nos EUA na década de 1970 quando pesquisas revelaram que os resíduos tóxicos eram destinados de forma desproporcional à populações afrodescendentes. A agroecologia, campo de produção científico interdisciplinar, também se consolidou na década de 1970, oriunda da fusão das ciências naturais e sociais, com o propósito de permitir o estilos de agricultura com maiores níveis de sustentabilidade, socialmente justa e economicamente viável. Esse trabalho situa-se no campo da pesquisa teórica, de caráter exploratório sobre as concepções e convergências da área da justiça ambiental e da agroecologia, utilizando-se de uma pesquisa bibliográfica com abordagem qualitativa. Ao fortalecer os modos de vida camponeses, a agroecologia tem contribuído na luta por justiça ambiental. Nesse sentido, podemos perceber a relação entre o movimento agroecológico e o movimento por justiça ambiental, dada suas convergências no objetivo de promover a justiça social.The movement for environmental justice emerged in the US in the 1970s when research revealed that toxic waste was allocated proportional to the Afro-descendant populations.
Agroecology, interdisciplinary scientific production field, also consolidated in the 1970s, derived from the fusion of natural and social sciences, in order to allow the farming styles with higher levels of sustainability, socially just and economically viable. This work lies in the field of theoretical research, exploratory on the concepts and similarities of the area of environmental justice and agroecology, using a literature research with qualitative approach.
By strengthening livelihoods farmers, agroecology has contributed in the struggle for environmental justice. In this sense, we can see the relationship between agro-ecological movement and the movement for environmental justice, given their convergence in order to promote social justice.Eje A6: Desarrollo Rural, Movimientos Sociales, Estado y AgroecologíaFacultad de Ciencias Agrarias y Forestale
Agroecologia e justiça ambiental: um diálogo possível
O movimento por justiça ambiental surgiu nos EUA na década de 1970 quando pesquisas revelaram que os resíduos tóxicos eram destinados de forma desproporcional à populações afrodescendentes. A agroecologia, campo de produção científico interdisciplinar, também se consolidou na década de 1970, oriunda da fusão das ciências naturais e sociais, com o propósito de permitir o estilos de agricultura com maiores níveis de sustentabilidade, socialmente justa e economicamente viável. Esse trabalho situa-se no campo da pesquisa teórica, de caráter exploratório sobre as concepções e convergências da área da justiça ambiental e da agroecologia, utilizando-se de uma pesquisa bibliográfica com abordagem qualitativa. Ao fortalecer os modos de vida camponeses, a agroecologia tem contribuído na luta por justiça ambiental. Nesse sentido, podemos perceber a relação entre o movimento agroecológico e o movimento por justiça ambiental, dada suas convergências no objetivo de promover a justiça social.The movement for environmental justice emerged in the US in the 1970s when research revealed that toxic waste was allocated proportional to the Afro-descendant populations.
Agroecology, interdisciplinary scientific production field, also consolidated in the 1970s, derived from the fusion of natural and social sciences, in order to allow the farming styles with higher levels of sustainability, socially just and economically viable. This work lies in the field of theoretical research, exploratory on the concepts and similarities of the area of environmental justice and agroecology, using a literature research with qualitative approach.
By strengthening livelihoods farmers, agroecology has contributed in the struggle for environmental justice. In this sense, we can see the relationship between agro-ecological movement and the movement for environmental justice, given their convergence in order to promote social justice.Eje A6: Desarrollo Rural, Movimientos Sociales, Estado y AgroecologíaFacultad de Ciencias Agrarias y Forestale
Implicações do desenvolvimento econômico no trabalho, ambiente e saúde em comunidades portuárias no Ceará, Brasil
Saúde, ambiente e sustentabilidade.
Colaboradores: Ada Cristina Pontes Aguiar; Guilherme Costa Delgado; Horácio Martins de Carvalho; Lucas Resende; Marcelo Firpo de Souza Porto; Núcleo Tramas; Pedro Costa Cavalcanti de Albuquerque; Vanira Matos Pessoa; Veruska Prado Alexandre; Vicente Eduardo Soares de Almeida, CNPH
The precarious livelihood in waste dumps: a report on food insecurity and hunger among recyclable waste collectors
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