Hypotherme Konservierung von Ebersperma bei 5°C : Ein Praxisversuch

Hypothermic preservation of boar semen at 5°C instead of 17°C is a new approach to reduce antibiotics in semen doses. For practical use, information on the potentially boar-individual response to semen chilling and in vivo fertility is decisive. The aim was to assess sperm quality after long-term storage at 5°C in four consecutive ejaculates of 20 boars and to test for fertility in a pretrial prior to a large-scale field study. In samples stored at 5°C in Androstar Premium and ana-lysed at 24, 72, 96 and 144 h, overall motility assessed with computer- assisted semen analysis was 79.9% compared to 81.1% in controls stored at 17°C in Beltsville Thawing Solution. Two boars had lower sperm motility in 5°C samples compared to controls (p .05) between the storage groups showing 6.5% defects at 5°C and 7.1% in control samples at 144 h. Insemination with pooled semen of three boars stored split sample at 5°C (n = 89 sows) and 17°C (n = 91 sows) yielded high fertility. There were no differences (p > .05) in farrowing rate (5°C: 92.1%; 17°C: 92.3%) and average number of born piglets (5°C: 18.7 ± 3.6; 17°C: 18.5 ± 2.8). In conclusion, sperm quality in samples stored at 5°C was maintained in all boars above thresholds for useable semen as defined by the German Livestock Association (BRS e.V.). First field data with 5°C-boar semen in Europe indicate that hypothermic stor-age is applicable in routine insemination

Fertility Preservation for Patients with Malignant Disease. Guideline of the DGGG, DGU and DGRM (S2k-Level, AWMF Registry No. 015/082, November 2017) - Recommendations and Statements for Girls and Women.

Aim The aim of this official guideline published by the German Society of Gynecology and Obstetrics (DGGG) and coordinated with the German Society of Urology (DGU) and the German Society of Reproductive Medicine (DGRM) is to provide consensus-based recommendations, obtained by evaluating the relevant literature, on counseling and fertility preservation for prepubertal girls and boys as well as patients of reproductive age. Statements and recommendations for girls and women are presented below. Statements or recommendations for boys and men are not the focus of this guideline. Methods This S2k guideline was developed at the suggestion of the guideline commission of the DGGG, DGU and DGRM and represents the structured consensus of representative members from various professional associations (n = 40). Recommendations The guideline provides recommendations on counseling and fertility preservation for women and girls which take account of the patient's personal circumstances, the planned oncologic therapy and the individual risk profile as well as the preferred approach for selected tumor entities