26 research outputs found
Biological characteristics of Bovine Herpesvirus 1 and 5 strains using the rabbit experimental model
Bovine Herpesvirus (BoHV) can infect both rabbits and mustelids. Rabbit has been used as a laboratory model for infection with BoHV-1 and 5. The objective of this research was to study the pathogenicity of different Argentinian BoHV-1 and BoHV-5 strains by using the rabbit experimental model. New Zealand rabbits were inoculated by intranasal and intravaginal ways. The animals inoculated intranasally with strains of BoHV-5 developed neurological signs in 83% of the cases. BoHV-1.1 caused neurological signs in 57% of the animals and BoHV-1.2 did not cause clear clinical signs. BoHV-5 caused nervous signs in young animals while BoHV-1 did so occasionally in young rabbits. Animales inoculated intravaginally showed no apparent clinical signs or apparent lesions in the studied organs. The infection was demonstrated by serological seroconversion. The rabbit was appropriate to study the clinical signs and the lesions produced in the different organs, primarily in the central nervous system. The model was useful for being inexpensive and very easy to use, and it enabled to identify differences in the biological behavior of the studied BoHV-1 and BoHV-5 strains.Fil: Pidone, C.L. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Santa Fe, ArgentinaFil: Riganti, J.G. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Santa Fe, ArgentinaFil: Valera, A.R. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. La Plata, ArgentinaFil: Poli, G.L. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Santa Fe, ArgentinaFil: Ridley, A.I. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Santa Fe, ArgentinaFil: Fuentealba, N.A. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. La Plata, ArgentinaFil: Fuentealba, N.A. CONICET. Centro Cient铆fico Tecnol贸gico (CCT). La Plata, ArgentinaFil: Anthony, L.M. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Santa Fe, ArgentinaFil: Brion, C. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Santa Fe, ArgentinaFil: Pereyra, N.B. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Santa Fe, ArgentinaFil: Galosi, C.M. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. La Plata, ArgentinaFil: Galosi, C.M. Comisi贸n de Investigaciones Cient铆ficas (CIC). La Plata, ArgentinaLos Herpesvirus bovinos (BoHV) pueden infectar tanto a must茅lidos como a conejos y esta 煤ltima especie ha sido utilizada como modelo de laboratorio para la infecci贸n por BoHV-1 y 5. El objetivode este trabajo fue estudiar la patogenicidad de diferentes cepas argentinas de BoHV-1 y BoHV-5 utilizando el modelo experimental conejo. Se utilizaron conejos de raza neozelandesa que se inocularon por v铆a intranasal e intravaginal. Los animales inoculados por v铆a intranasal con cepas de BoHV-5 desarrollaron signos nerviosos en el 83% de los casos, mientras que BoHV-1.1 caus贸 signos nerviosos en el 57% de los animales y BoHV-1.2 no provoc贸 signos cl铆nicos evidentes. El BoHV-5 caus贸 s铆ntomas nerviosos solo en los animales j贸venes mientras que BoHV-1 solo lo hizo ocasionalmente y tambi茅n en individuos j贸venes. Los conejos inoculados por v铆a intravaginal no mostraron signos cl铆nicos ni lesiones aparentes en los 贸rganos estudiados; la infecci贸n se demostr贸 por seroconversi贸n serol贸gica. El conejo result贸 adecuado para estudiar la sintomatolog铆a y las lesiones producidas en los distintos 贸rganos, fundamentalmente en el sistema nervioso central. El modelo result贸 de utilidad por ser econ贸mico, de muy f谩cil manejo y permiti贸 reconocer diferencias en el comportamiento biol贸gico de las cepas de BoHV-1 y BoHV-5 estudiadas
Both the stroma and thylakoid lumen of tobacco chloroplasts are competent for the formation of disulphide bonds in recombinant proteins
International audienc
Both the stroma and thylakoid lumen of tobacco chloroplasts are competent for the formation of disulphide bonds in recombinant proteins
The evolution of repeated mating under sexual conflict
In insects, repeated mating by females may have direct effects on female fecundity, fertility, and longevity. In addition, a female's remating rate affects her fitness through mortality costs of male harassment and ecological risks of mating such as predation. We analyse a model where these female fitness factors are put into their life-history context, and traded against each other, while accounting for limitations because of mate availability. We solve analytically for the condition when female multiple mating will evolve. We show that the probability that a female mates with a courting male decreases with increases in population density. The extent of conflict between the sexes thus automatically becomes larger at higher densities. However, because at higher densities females meet males at a higher rate, the resulting ESS female remating rate is independent of population density. The female remating probability is in conflict with male adaptations that increase male mating rate by persuading or forcing females to mate, and also in conflict with male adaptations for protecting the own sperm from being removed by future female mates. We show that the relative importance of these conflicts depends on population density