8 research outputs found
Cardiorespiratory fitness and sports activities in children and adolescents with solitary functioning kidney
Background: An increasing number of children with chronic disease require a complete medical examination to
be able to practice physical activity. Particularly children with solitary functioning kidney (SFK) need an accurate
functional evaluation to perform sports activities safely. The aim of our study was to evaluate the influence of regular
physical activity on the cardiorespiratory function of children with solitary functioning kidney.
Method: Twenty-nine patients with congenital SFK, mean age 13.9 ± 5.0 years, and 36 controls (C), mean age
13.8 ± 3.7 years, underwent a cardiorespiratory assessment with spirometry and maximal cardiopulmonary exercise
testing. All subjects were divided in two groups: sedentary (S) and trained (T) patients, by means of a standardized
questionnaire about their weekly physical activity.
Results: We found that mean values of maximal oxygen consumption (VO2max) and exercise time (ET) were higher in
T subjects than in S subjects. Particularly SFK-T presented mean values of VO2max similar to C-T and significantly higher
than C-S (SFK-T: 44.7 ± 6.3 vs C-S: 37.8 ± 3.7 ml/min/kg; p < 0.0008). We also found significantly higher mean values of
ET (minutes) in minutes in SFK-T than C-S subjects (SFK-T: 12.9 ± 1.6 vs C-S: 10.8 ± 2.5 min; p <0.02).
Conclusion: Our study showed that regular moderate/high level of physical activity improve aerobic capacity (VO2max)
and exercise tolerance in congenital SFK patients without increasing the risks for cardiovascular accidents and accordingly
sports activities should be strongly encouraged in SFK patients to maximize health benefit
Cardiorespiratory fitness and sports activities in children and adolescents with solitary functioning kidney
Background: An increasing number of children with chronic disease require a complete medical examination to be able to practice physical activity. Particularly children with solitary functioning kidney (SFK) need an accurate functional evaluation to perform sports activities safely. The aim of our study was to evaluate the influence of regular physical activity on the cardiorespiratory function of children with solitary functioning kidney.
Method: Twenty-nine patients with congenital SFK, mean age 13.9 ± 5.0 years, and 36 controls (C), mean age 13.8 ± 3.7 years, underwent a cardiorespiratory assessment with spirometry and maximal cardiopulmonary exercise testing. All subjects were divided in two groups: sedentary (S) and trained (T) patients, by means of a standardized questionnaire about their weekly physical activity.
Results: We found that mean values of maximal oxygen consumption (VO2max) and exercise time (ET) were higher in T subjects than in S subjects. Particularly SFK-T presented mean values of VO2max similar to C-T and significantly higher than C-S (SFK-T: 44.7 ± 6.3 vs C-S: 37.8 ± 3.7 ml/min/kg; p < 0.0008). We also found significantly higher mean values of ET (minutes) in minutes in SFK-T than C-S subjects (SFK-T: 12.9 ± 1.6 vs C-S: 10.8 ± 2.5 min; p <0.02).
Conclusion: Our study showed that regular moderate/high level of physical activity improve aerobic capacity (VO2max) and exercise tolerance in congenital SFK patients without increasing the risks for cardiovascular accidents and accordingly sports activities should be strongly encouraged in SFK patients to maximize health benefits
Effect of Bisphenol A with or without enzyme treatment on the proliferation and viability of MCF-7 cells
Recently, aqueous solutions polluted by BPA have been bioremediated by us using laccase immobilized on hydrophobic membranes in non-isothermal bioreactors. BPA degradation was checked using analytical
methods. To assess in vitro the occurred bioremediation, the proliferation and viability indexes of MCF-7 cells
incubated in the presence of aqueous solutions of BPA , or of enzyme-treated BPA solutions, have been
measured as a fu nction of the initial BPA concentration.
The results demonstrated that:
i) at each initial BPA concentration used, both the proliferation and viability indexes are a function of the
duration of enzyme treatment;
ii) proliferation and viability are uncoupled biological processes with respect to BPA enzyme treatment.
Non-isothermal bioreactors are a useful tool for the bioremediation of aqueous solutions polluted by BPA,
which is an example of an endocrine disruptor that belongs to the alkyl phenol famil
Nonisothermal bioreactors in enzymatic remediation of waters polluted by endocrine disruptors: the BPA as model of pollutant
The bioremediation of waters polluted by Bisphenol A, taken as a model for endocrine disruptors, has been pursued by means of catalytic
membranes in bioreactors operating under isothermal and non-isothermal conditions. Laccase from Trametes versicolor was immobilized on nylon
membranes grafted with Glycidyl Methacrylate and using Phenylendiamine as spacer. The behaviour of the catalytic membrane was studied as a
function of BPA concentration. Affinities of immobilized laccase towards BPA were found to increase with average temperature and under nonisothermal
conditions. Percentage increases of enzyme activity, proportional to the applied temperature differences, were found to decrease with
the increase of BPA concentrations. Interestingly, the highest levels of BPA biodegradation occurred at the lowest concentrations, in other words
those present in wastewaters given the small water solubility of this compound. The results are discussed in terms of the process of thermodialysis
by considering the additional BPA fluxes towards the immobilized enzymes driven by the temperature gradients