Análise do processo de reparo do reimplante de incisivos de ratos diabéticos após a manutenção dos dentes em leite

O prognóstico de um dente reimplantado está relacionado a integridade do ligamento periodontal cementário e da camada de cemento. Esta, por sua vez, e influenciada por vários fatores, sendo os principais, o periodo extra-alveolar e o meio de conservação. Existem dúvidas, no entanto, se alterações sistêmicas, como o diabetes, também podem interferir no prognóstico do reimplante dentário. Portanto, o propósito deste trabalho foi avaliar o processo de reparo do reimplante de incisivos de ratos diabéticos nao controlados após a manutenção dos dentes em leite bovino pasteurizado. Para tanto, 32 ratos foram divididos em dois grupos de 16 animais cada, sendo que no Grupo I foi administrada, por via endovenosa, injeção de tampão citrato e no Grupo II inoculada estreptozotocina dissolvida em tampão citrato pela mesma via, para a indução do diabetes. Após controle glicêmico e a comprovação das altas taxas de glicemia, o incisivo superior direito de cada animal foi extraído e mantido em leite durante sessenta minutos. Na sequência, realizou-se o tratamento endodontico com a colocação de curativo de hidróxido de cálcio e o posterior reimplante dos dentes em seus respectivos alveolos. A antibioticoterapia sistêmica foi instituida no pós-operatório imediato. Decorridos 10 e 60 dias após o reimplante, realizou-se a eutanasia dos animais e as peças obtidas foram submetidas ao processamento laboratorial para a obtenção dos cortes, os quais foram corados com hematoxilina e eosina e Tricromicro de Masson para análise histologica e histométrica. Os resultados demonstraram que o tecido conjuntivo adjacente a superfície radicular se apresentou desorganizado nos animais diabéticos. Ìndices menores de reabsorção radicular externa e anquilose também foram encontrados neste grupo, porém, sem diferenças estatísticas significantes.The prognosis of a replanted tooth is related to the integrity of the cemental periodontal ligament and the cementum layer, which, in turn, is influenced by several factors. Among these factors, the extra-alveolar time and the storage medium are the most important. However, it remains unclear whether systemic alterations, such as diabetes, may also interfere with the prognosis of tooth replantation. Therefore, the purpose of this investigation was therefore to evaluate the healing process of non-controlled diabetic rats' incisors replanted after storage of the teeth in bovine pasteurized milk. A total of 32 rats were assigned to two groups of 16 animals each. In group I, a citrate buffer injection was administered intravenously to rats while the animals in group II received an intravenous injection of streptozotocin dissolved in citrate buffer to induce diabetes. After confirmation of high glycemic levels, the maxillary right incisor of each animal was extracted and stored in milk during 60 minutes. Thereafter, the teeth were endodontically treated, with placement of a calcium hydroxide-based intracanal dressing, and the teeth replanted into their sockets. Systemic antibiotic therapy started in the immediate postoperative period. The animals were euthanized 10 and 60 days postoperatively. The anatomical pieces were removed, submitted to routine laboratorial processing and serially sectioned to obtain cuts that were stained by hematoxylin and eosin and Masson's Trichrome for histologic and histometric analyses. The results showed that the connective tissue adjacent to the root surface was disorganized in the diabetic rats. Lower indices of external root resorption and ankylosis were also observed in the diabetic group, though with no statistically significant difference.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

Assistência cirúrgico-odontológica a pacientes com história de infarto do miocárdio

The execution of a appropriate odontological surgical treatment for patients with Myocardial Infarction has been based in established protocols for important works in world wide literature during the sixty’s and seventy’s decade, established with a minimum of six months before the noncardiac surgery. The recent advancements in the medical assessment of surgical risk based on the individual analysis of each case with availability of new therapeutic tools in reference to medical or dental treatment, allowing for new procedures that can be used in the execution of safer odontological surgery for each patients. This review of the literature shows the actual tendencies of the treatment of patients with Myocardial Infarction concerning specific care before, during and after surgery based on laboratory evaluation and drug therapy as well as the possibility of surgical treatment before six months post-infarction.A execução de um plano de tratamento cirúrgico-odontológico adequado em pacientes com história de Infarto do Miocárdio tem sido baseada em protocolos estabelecidos desde as décadas de 1960 e 1970, que estipulavam um período de espera mínimo de seis meses para a execução de cirurgia não-cardíaca. Atualmente, os recentes avanços na avaliação médica do risco cirúrgico com base na análise individual de cada caso, bem como a disponibilidade de novos recursos terapêuticos tanto no que se refere ao tratamento médico quanto odontológico, permitem que novas posturas sejam adotadas na execução de um cirurgia odontológica segura nestes pacientes. Neste trabalho, a partir da revisão de literatura, procurou-se abordar as tendências atuais do tratamento de pacientes com história de infarto agudo do miocárdio, no que se refere aos cuidados específicos durante o pré, trans e pós-operatório, tendo em vista a avaliação laboratorial e terapêutica medicamentosa; bem como na possibilidade de tratamento cirúrgico-odontológico prévio aos seis meses pós-infarto

Healing process of incisor teeth of diabetic rats replanted after storage in milk

Several local factors that influence the healing process of replanted teeth have been investigated. However, it remains unclear how systemic alterations, such as diabetes mellitus, affect the prognosis of these cases. The purpose of this study was to evaluate the healing process of incisors of non-controlled diabetic rats replanted after storage in bovine long shelf-life (UHT) whole milk. Thirty-two rats were randomly assigned to receive an endovenous injection of either citrate buffer solution (group I - control; n = 16) or streptozotocin dissolved in citrate buffer solution to induce diabetes (group II; n = 16). After confirmation of the diabetic status by analysis of the glycemic levels, the maxillary right incisor of each animal was extracted and immersed in milk for 60 min. The root canals of teeth were then instrumented, and were filled with a calcium hydroxide-based dressing and replanted into their sockets. All animals received systemic antibiotic and were killed by anesthetic overdose 10 and 60 days after replantation. The specimens containing the replanted teeth were removed, fixed, decalcified, and embedded in paraffin. Semi-serial 6-mu m-thick sections were obtained and stained with hematoxylin and eosin for histologic and histometric analyses. The results showed that the connective tissue adjacent to the root surface was less organized in the diabetic animals than in the control animals in both periods; the root dentin was less severely affected by root resorption in the diabetic rats; there were no significant differences between the control and diabetic groups regarding the occurrence of replacement resorption and inflammatory resorption