A Giant Outburst at Millimeter Wavelengths in the Orion Nebula

BIMA observations of the Orion nebula discovered a giant flare from a young star previously undetected at millimeter wavelengths. The star briefly became the brightest compact object in the nebula at 86 GHz. Its flux density increased by more than a factor of 5 on a timescale of hours, to a peak of 160 mJy. This is one of the most luminous stellar radio flares ever observed. Remarkably, the Chandra X-ray observatory was in the midst of a deep integration of the Orion nebula at the time of the BIMA discovery; the source's X-ray flux increased by a factor of 10 approximately 2 days before the radio detection. Follow-up radio observations with the VLA and BIMA showed that the source decayed on a timescale of days, then flared again several times over the next 70 days, although never as brightly as during the discovery. Circular polarization was detected at 15, 22, and 43 GHz, indicating that the emission mechanism was cyclotron. VLBA observations 9 days after the initial flare yield a brightness temperature Tb > 5 x 10^7 K at 15 GHz. Infrared spectroscopy indicates the source is a K5V star with faint Br gamma emission, suggesting that it is a weak-line T Tauri object. Zeeman splitting measurements in the infrared spectrum find B ~ 2.6 +/- 1.0 kG. The flare is an extreme example of magnetic activity associated with a young stellar object. These data suggest that short observations obtained with ALMA will uncover hundreds of flaring young stellar objects in the Orion region.Comment: 29 pages, 7 figures, accepted for publication in Ap

Deep Investigation of Neutral Gas Origins (DINGO): HI stacking experiments with early science data

We present early science results from Deep Investigation of Neutral Gas Origins (DINGO), an HI survey using the Australian Square Kilometre Array Pathfinder (ASKAP). Using ASKAP sub-arrays available during its commissioning phase, DINGO early science data were taken over $\sim$ 60 deg$^{2}$ of the Galaxy And Mass Assembly (GAMA) 23 h region with 35.5 hr integration time. We make direct detections of six known and one new sources at $z < 0.01$. Using HI spectral stacking, we investigate the HI gas content of galaxies at $0.04 < z< 0.09$ for different galaxy colours. The results show that galaxy morphology based on optical colour is strongly linked to HI gas properties. To examine environmental impacts on the HI gas content of galaxies, three sub-samples are made based on the GAMA group catalogue. The average HI mass of group central galaxies is larger than those of satellite and isolated galaxies, but with a lower HI gas fraction. We derive a variety of HI scaling relations for physical properties of our sample, including stellar mass, stellar mass surface density, $NUV-r$ colour, specific star formation rate, and halo mass. We find that the derived HI scaling relations are comparable to other published results, with consistent trends also observed to $\sim$0.5 dex lower limits in stellar mass and stellar surface density. The cosmic HI densities derived from our data are consistent with other published values at similar redshifts. DINGO early science highlights the power of HI spectral stacking techniques with ASKAP.Comment: 27 pages, 25 figures, 10 tables, accepted for publication in MNRA

Deep Investigation of Neutral Gas Origins (DINGO): HI stacking experiments with early science data

We present early science results from Deep Investigation of Neutral Gas Origins (DINGO), an H I survey using the Australian Square Kilometre Array Pathfinder (ASKAP). Using ASKAP subarrays available during its commissioning phase, DINGO early science data were taken over ∼60 deg2 of the Galaxy And Mass Assembly (GAMA) 23 h region with 35.5 h integration time. We make direct detections of six known and one new sources at z \u3c 0.01. Using H I spectral stacking, we investigate the H I gas content of galaxies at 0.04 \u3c z \u3c 0.09 for different galaxy colours. The results show that galaxy morphology based on optical colour is strongly linked to H I gas properties. To examine environmental impacts on the H I gas content of galaxies, three subsamples are made based on the GAMA group catalogue. The average H I mass of group central galaxies is larger than those of satellite and isolated galaxies, but with a lower H I gas fraction. We derive a variety of H I scaling relations for physical properties of our sample, including stellar mass, stellar mass surface density, NUV − r colour, specific star formation rate, and halo mass. We find that the derived H I scaling relations are comparable to other published results, with consistent trends also observed to ∼0.5 dex lower limits in stellar mass and stellar surface density. The cosmic H I densities derived from our data are consistent with other published values at similar redshifts. DINGO early science highlights the power of H I spectral stacking techniques with ASKA

The Murchison Widefield Array

It is shown that the excellent Murchison Radio-astronomy Observatory site allows the Murchison Widefield Array to employ a simple RFI blanking scheme and still calibrate visibilities and form images in the FM radio band. The techniques described are running autonomously in our calibration and imaging software, which is currently being used to process an FM-band survey of the entire southern sky.Comment: Accepted for publication in Proceedings of Science [PoS(RFI2010)016]. 6 pages and 3 figures. Presented at RFI2010, the Third Workshop on RFI Mitigation in Radio Astronomy, 29-31 March 2010, Groningen, The Netherland

Early dysregulation of cardiac-specific microRNA-208a is linked to maladaptive cardiac remodelling in diabetic myocardium

Abstract Background The diabetic heart undergoes remodelling contributing to an increased incidence of heart failure in individuals with diabetes at a later stage. The molecular regulators that drive this process in the diabetic heart are still unknown. Methods Real-time (RT) PCR analysis was performed to determine the expression of cardiac specific microRNA-208a in right atrial appendage (RAA) and left ventricular (LV) biopsy tissues collected from diabetic and non-diabetic patients undergoing coronary artery bypass graft surgery. To determine the time-dependent changes, cardiac tissue were collected from type 2 diabetic mice at different age groups. A western blotting analysis was conducted to determine the expression of contractile proteins α- and β-myosin heavy chain (MHC) and thyroid hormone receptor-α (TR-α), the negative regulator of β-MHC. To determine the beneficial effects of therapeutic modulation of miR-208a, high glucose treated adult mouse HL-1 cardiomyocytes were transfected with anti-miR-208a. Results RT-PCR analysis showed marked upregulation of miR-208a from early stages of diabetes in type 2 diabetic mouse heart, which was associated with a marked increase in the expression of pro-hypertrophic β-MHC and downregulation of TR-α. Interestingly, upregulation of miR-208a preceded the switch of α-/β-MHC isoforms and the development of diastolic and systolic dysfunction. We also observed significant upregulation of miR-208a and modulation of miR-208a associated proteins in the type 2 human diabetic heart. Therapeutic inhibition of miR-208a activity in high glucose treated HL-1 cardiomyocytes prevented the activation of β-MHC and hence the hypertrophic response. Conclusion Our results provide the first evidence that early modulation of miR-208a in the diabetic heart induces alterations in the downstream signaling pathway leading to cardiac remodelling and that therapeutic inhibition of miR-208a may be beneficial in preventing diabetes-induced adverse remodelling of the heart

Thiamine increases resident endoglin positive cardiac progenitor cells and atrial contractile force in humans:A randomised controlled trial

Background: The heart has an intrinsic ability to regenerate, orchestrated by progenitor or stem cells. However, the relative complexity of non-resident cardiac progenitor cell (CPC) therapy makes modulation of resident CPCs a more attractive treatment target. Thiamine analogues improve resident CPC function in pre-clinical models. In this double blinded randomised controlled trial (identifier: ACTRN12614000755639), we examined whether thiamine would improve CPC function in humans. Methods and results: High dose oral thiamine (one gram twice daily) or matching placebo was administered 3–5 days prior to coronary artery bypass surgery (CABG). Right atrial appendages were collected at the time of CABG, and CPCs isolated. There was no difference in the primary outcome (proliferation ability of CPCs) between treatment groups. Older age was not associated with decreased proliferation ability. In exploratory analyses, isolated CPCs in the thiamine group showed an increase in the proportion of CD34−/CD105+ (endoglin) cells, but no difference in CD34−/CD90+ or CD34+ cells. Thiamine increased maximum force developed by isolated trabeculae, with no difference in relaxation time or beta-adrenergic responsiveness. Conclusion: Thiamine does not improve proliferation ability of CPC in patients undergoing CABG, but increases the proportion of CD34−/CD105+ cells. Having not met its primary endpoint, this study provides the impetus to re-examine CPC biology prior to any clinical outcome-based trial examining potential beneficial cardiovascular effects of thiamine.</p

The UTMOST: A Hybrid Digital Signal Processor Transforms the Molonglo Observatory Synthesis Telescope

The Molonglo Observatory Synthesis Telescope (MOST) is an 18000 m2 radio telescope located 40 km from Canberra, Australia. Its operating band (820–851 MHz) is partly allocated to telecommunications, making radio astronomy challenging. We describe how the deployment of new digital receivers, Field Programmable Gate Array-based filterbanks, and server-class computers equipped with 43 Graphics Processing Units, has transformed the telescope into a versatile new instrument (UTMOST) for studying the radio sky on millisecond timescales. UTMOST has 10 times the bandwidth and double the field of view compared to the MOST, and voltage record and playback capability has facilitated rapid implementaton of many new observing modes, most of which operate commensally. UTMOST can simultaneously excise interference, make maps, coherently dedisperse pulsars, and perform real-time searches of coherent fan-beams for dispersed single pulses. UTMOST operates as a robotic facility, deciding how to efficiently target pulsars and how long to stay on source via real-time pulsar folding, while searching for single pulse events. Regular timing of over 300 pulsars has yielded seven pulsar glitches and three Fast Radio Bursts during commissioning. UTMOST demonstrates that if sufficient signal processing is applied to voltage streams, innovative science remains possible even in hostile radio frequency environments

Data supporting the activation of autophagy genes in the diabetic heart

This data article contains full list of autophagy related genes that are altered in diabetic heart. This article also shows data from in vitro cultured cardiomyocytes that are exposed the high glucose treatment to simulate hyperglycemic state in vitro. The interpretation of these data and further extensive insights into the regulation of SG biogenesis by AMPK can be found in “Type-2 diabetes increases autophagy in the human heart through promotion of Beclin-1 mediated pathway” (Munasinghe et al., in press) [1]

Type-2 diabetes increases autophagy in the human heart through promotion of Beclin-1 mediated pathway

Background: Diabetes promotes progressive loss of cardiac cells, which are replaced by a fibrotic matrix, resulting in the loss of cardiac function. In the current study we sought to identify if excessive autophagy plays a major role in inducing this progressive loss.Methods and results: Immunofluorescence and western blotting analysis of the right atrial appendages collected from diabetic and non-diabetic patients undergoing coronary artery bypass graft surgery showed a marked increase in the level of autophagy in the diabetic heart, as evidenced by increased expression of autophagy marker LC3B-II and its mediator Beclin-1 and decreased expression of p62, which incorporates into autophagosomes to be efficiently degraded. Moreover, a marked activation of pro-apoptotic caspase-3 was observed. Electron microscopy showed increased autophagosomes in the diabetic heart. In vivo measurement of autophagic flux by choloroquine injection resulted in further enhancement of LC3B-II in the diabetic myocardium, confirming increased autophagic activity in the type-2 diabetic heart. Importantly, in-vitro genetic depletion of beclin-1 in high glucose treated adult rat cardiomyocytes markedly inhibited the level of autophagy and subsequent apoptotic cell death.Conclusions: These findings demonstrate the pathological role of autophagy in the type-2 diabetic heart, opening up a potentially novel therapeutic avenue for the treatment of diabetic heart disease