Prolonging Kidney Graft Survival with Concanavalin A: Effects of temperature, perfusate composition, pH, and different manufacturing lots

This study analyzes the effect of temperature, perfusate composition, pH, and variable manufacturing lots in prolonging kidney allograft survival with Concanavalin A (Con A). Cold temperature (4°C), crystalloid composition of the perfusates, and neutral or mildly alkaline pH were important factors in the effect of Con A on prolonging allograft survival. Also, different lots of Con A from the same manufacturer produced variable results in prolonging survival. Thus, multiple factors should be considered if Con A is to be used to prolong kidney allograft survival

Leaf-Encapsulated Vaccines: Agroinfiltration and Transient Expression of the Antigen Staphylococcal Endotoxin B in Radish Leaves.

Transgene introgression is a major concern associated with transgenic plant-based vaccines. Agroinfiltration can be used to selectively transform nonreproductive organs and avoid introgression. Here, we introduce a new vaccine modality in which Staphylococcal enterotoxin B (SEB) genes are agroinfiltrated into radishes (Raphanw sativus L.), resulting in transient expression and accumulation of SEB in planta. This approach can simultaneously express multiple antigens in a single leaf. Furthermore, the potential of high-throughput vaccine production was demonstrated by simultaneously agroinfiltrating multiple radish leaves using a multichannel pipette. The expression of SEB was detectable in two leaf cell types (epidermal and guard cells) in agroinfiltrated leaves. ICR mice intranasally immunized with homogenized leaves agroinfiltrated with SEB elicited detectable antibody to SEB and displayed protection against SEB-induced interferon-gamma (IFN-γ) production. The concept of encapsulating antigens in leaves rather than purifying them for immunization may facilitate rapid vaccine production during an epidemic disease

A New Channel for the Detection of Planetary Systems Through Microlensing: II. Repeating Events

In the companion paper we began the task of systematically studying the detection of planets in wide orbits ($a > 1.5 R_E$) via microlensing surveys. In this paper we continue, focusing on repeating events. We find that, if all planetary systems are similar to our own Solar System, reasonable extensions of the present observing strategies would allow us to detect 3-6 repeating events per year along the direction to the Bulge. Indeed, if planetary systems with multiple planets are common, then future monitoring programs which lead to the discovery of thousands of stellar-lens events will likely discover events in which several different planets within a single system serve as lenses, with light curves exhibiting multiple repetitions. In this paper we discuss observing strategies to maximize the discovery of all wide-orbit planet-lens events. We also compare the likely detection rates of planets in wide orbits to those of planets located in the zone for resonant lensing. We find that, depending on the values of the planet masses and stellar radii of the lensed sources (which determine whether or not finite source size is important), and also on the sensitivity of the photometry used by observers, the detection of planets in wide orbits may be the primary route to the discovery of planets via microlensing. We also discuss how the combination of resonant and wide-orbit events can help us to learn about the distribution of planetary system properties (S 6.1). In addition, by determining the fraction of short-duration events due to planets, we indirectly derive information about the fraction of all short-duration events that may be due to low-mass MACHOs (S 6.2).Comment: 51 pages, 7 figures. To be published in the Astrophysical Journal, 20 February 1999. This completes the introduction to the discovery of planets in wide orbits begun in astro-ph/9808075, also to appear in ApJ on 20 February 199

A New Channel for the Detection of Planetary Systems Through Microlensing: I. Isolated Events Due to Planet Lenses

We propose and evaluate the feasibility of a new strategy to search for planets via microlensing. This new strategy is designed to detect planets in "wide" orbits, i.e., with orbital separation, $a$ greater than $\sim 1.5 R_E$. Planets in wide orbits may provide the dominant channel for the microlensing discovery of planets, particularly low-mass (e.g., Earth-mass) planets. This paper concentrates on events in which a single planet serves as a lens, leading to an isolated event of short duration. We point out that a distribution of events due to lensing by stars with wide-orbit planets is necessarily accompanied by a distribution of shorter- duration events. The fraction of events in the latter distribution is proportional to the average value of $\sqrt{q}$, where $q$ is the ratio between \pl and stellar masses. The position of the peak or peaks also provides a measure of the mass ratios typical of planetary systems. We study detection strategies that can optimize our ability to discover isolated short-duration events due to lensing by planets, and find that monitoring employing sensitive photometry is particularly useful. If planetary systems similar to our own are common, even modest changes in detection strategy should lead to the discovery of a few isolated events of short duration every year. We therefore also address the issue of the contamination due to stellar populations of any microlensing signal due to low-mass MACHOs. We describe how, even for isolated events of short duration, it will be possible to test the hypothesis that the lens was a planet instead of a low-mass MACHO, if the central star of the planetary system contributes a measurable fraction of the baseline flux.Comment: 37 pages, 6 figure. To be published in the Astrophysical Journal. This is part one of a series of papers on microlensing by planetary systems containing wide-orbit planets; the series represents a reorganization and extension of astro-ph/971101

Blood and marrow transplantation compensation: Perspective in payer and provider relations

AbstractThe high cost per patient of hematopoietic cell transplantation (HCT) causes this therapy to be the focus of much controversy, given the competing societal demands to provide all possible therapy to preserve life while simultaneously limiting global health care expenditures. Treatment and eligibility decisions for HCT often are heavily scrutinized by both governmental and private payers and not simply determined by physicians, facility providers, and the patient. In an effort to control costs, payers have administrative infrastructure to review resource utilization by these patients. Additionally payers have developed payment methodologies, usually in the form of a case rate payment structure, that place facilities and physician providers of HCT at financial risk for adverse patient financial outcomes in an effort to promote optimal utilization and selection of patients for HCT. As providers enter into such financial risk arrangements with payers, the providers need to understand the true cost of care and be able to identify predictable and unpredictable outlier risks for the financial consequences of medical complications. HCT providers try to protect themselves from excessive financial risk by having different payment rates for different types of transplant, eg, autologous versus HLA or genotypically matched related versus HLA mismatched transplants. Because at certain times in the HCT process risk is more unpredictable, HCT providers require different payment system strategies for the different time periods of care such as evaluation, pre-transplant disease management, harvesting, and cell processing, as well as short- and long-term follow-up. Involvement by clinicians is essential for this process to be done well, especially given the rapid changes technological innovation brings to HCT. Constant dialogue and interaction between providers and payers on these difficult financial issues with HCT is essential to preserve patient access to this potentially lifesaving therapy

Laboratory Focus on Improving the Culture of Biosafety: Statewide Risk Assessment of Clinical Laboratories That Process Specimens for Microbiologic Analysis

The Wisconsin State Laboratory of Hygiene challenged Wisconsin laboratories to examine their biosafety practices and improve their culture of biosafety. One hundred three clinical and public health laboratories completed a questionnaire-based, microbiology-focused biosafety risk assessment. Greater than 96% of the respondents performed activities related to specimen processing, direct microscopic examination, and rapid nonmolecular testing, while approximately 60% performed culture interpretation. Although they are important to the assessment of risk, data specific to patient occupation, symptoms, and travel history were often unavailable to the laboratory and, therefore, less contributory to a microbiology-focused biosafety risk assessment than information on the specimen source and test requisition. Over 88% of the respondents complied with more than three-quarters of the mitigation control measures listed in the survey. Facility assessment revealed that subsets of laboratories that claim biosafety level 1, 2, or 3 status did not possess all of the biosafety elements considered minimally standard for their respective classifications. Many laboratories reported being able to quickly correct the minor deficiencies identified. Task assessment identified deficiencies that trended higher within the general (not microbiology-specific) laboratory for core activities, such as packaging and shipping, direct microscopic examination, and culture modalities solely involving screens for organism growth. For traditional microbiology departments, opportunities for improvement in the cultivation and management of highly infectious agents, such as acid-fast bacilli and systemic fungi, were revealed. These results derived from a survey of a large cohort of small- and large-scale laboratories suggest the necessity for continued microbiology-based understanding of biosafety practices, vigilance toward biosafety, and enforcement of biosafety practices throughout the laboratory setting

Entanglement in a Solid State Spin Ensemble

Entanglement is the quintessential quantum phenomenon and a necessary ingredient in most emerging quantum technologies, including quantum repeaters, quantum information processing (QIP) and the strongest forms of quantum cryptography. Spin ensembles, such as those in liquid state nuclear magnetic resonance, have been powerful in the development of quantum control methods, however, these demonstrations contained no entanglement and ultimately constitute classical simulations of quantum algorithms. Here we report the on-demand generation of entanglement between an ensemble of electron and nuclear spins in isotopically engineered phosphorus-doped silicon. We combined high field/low temperature electron spin resonance (3.4 T, 2.9 K) with hyperpolarisation of the 31P nuclear spin to obtain an initial state of sufficient purity to create a non-classical, inseparable state. The state was verified using density matrix tomography based on geometric phase gates, and had a fidelity of 98% compared with the ideal state at this field and temperature. The entanglement operation was performed simultaneously, with high fidelity, to 10^10 spin pairs, and represents an essential requirement of a silicon-based quantum information processor.Comment: 4 pages, 3 figures plus supporting information of 4 pages, 1 figure v2: Updated reference

Modulation of Na+/alanine cotransport in liver sinusoidal membrane vesicles by internal divalent cations

Rat liver basolateral plasma membrane (bILPM) vesicles resuspended in 5 mM Mg2+-, Ca2+-, Mn2+- or Co2+-containing media exhibited a markedly lower rate of Na+-stimulated -alanine transport. Divalent cation inhibition of -alanine uptake was dose dependent, and was observed only when the vesicles were pre-loaded with the divalent cations. The presence or absence of the metal ions in the extravesicular incubation media had no effect on -alanine transport. Conversely, pretreatment of the vesicles with 0.2 mM of either EGTA or EDTA resulted in higher initial rates of -alanine transport. This stimulation was overcome by addition of excess divalent cation to the vesicle suspension solution. Since these bILPM vesicles are primarily oriented right-side-out, the divalent cation inhibition of -alanine transport appears to be a result of their interaction with cytosolic components of the cell membrane. Total Na+ flux as measured with 22Na+ was not affected by intravesicular 5 mM Mg2+ or Ca2+, indicating that the inhibition was not due to dissipation of the Na+ gradient. These observations suggest that intracellular divalent cations may serve to modulate -alanine transport across the liver cell plasma membrane.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/28613/1/0000425.pd