2 research outputs found
Experimental Assessment of the Electronic and Geometrical Structure of a Near-Infrared Absorbing and Highly Fluorescent Microbial Rhodopsin
The recently discovered Neorhodopsin (NeoR) exhibits
absorption
and emission maxima in the near-infrared spectral region, which together
with the high fluorescence quantum yield makes it an attractive retinal
protein for optogenetic applications. The unique optical properties
can be rationalized by a theoretical model that predicts a high charge
transfer character in the electronic ground state (S0)
which is otherwise typical of the excited state S1 in canonical
retinal proteins. The present study sets out to assess the electronic
structure of the NeoR chromophore by resonance Raman (RR) spectroscopy
since frequencies and relative intensities of RR bands are controlled
by the ground and excited stateās properties. The RR spectra
of NeoR differ dramatically from those of canonical rhodopsins but
can be reliably reproduced by the calculations carried out within
two different structural models. The remarkable agreement between
the experimental and calculated spectra confirms the consistency and
robustness of the theoretical approach
Experimental Assessment of the Electronic and Geometrical Structure of a Near-Infrared Absorbing and Highly Fluorescent Microbial Rhodopsin
The recently discovered Neorhodopsin (NeoR) exhibits
absorption
and emission maxima in the near-infrared spectral region, which together
with the high fluorescence quantum yield makes it an attractive retinal
protein for optogenetic applications. The unique optical properties
can be rationalized by a theoretical model that predicts a high charge
transfer character in the electronic ground state (S0)
which is otherwise typical of the excited state S1 in canonical
retinal proteins. The present study sets out to assess the electronic
structure of the NeoR chromophore by resonance Raman (RR) spectroscopy
since frequencies and relative intensities of RR bands are controlled
by the ground and excited stateās properties. The RR spectra
of NeoR differ dramatically from those of canonical rhodopsins but
can be reliably reproduced by the calculations carried out within
two different structural models. The remarkable agreement between
the experimental and calculated spectra confirms the consistency and
robustness of the theoretical approach