Plasma concentrations (mean ± SEM) of estradiol (top panel) and progesterone (bottom panel) of cows in the LF/LCL group (n = 13) and in the SF/SCL group (n = 8).

<p>Within a given Day, significantly different means (P < 0.05) were indicated by an asterisk (*). LF/LCL, large follicle-large CL; SF/SCL, small follicle-small CL.</p

MA Plot showing ampulla (A; n = 5 samples) and isthmus (B; n = 6 samples) gene expression of LF/LCL and SF/SCL groups, in terms of the differentially expressed genes (p < 0.001).

<p>MA Plot showing ampulla (A; n = 5 samples) and isthmus (B; n = 6 samples) gene expression of LF/LCL and SF/SCL groups, in terms of the differentially expressed genes (p < 0.001).</p

Heat map constructed by clustering of the 50 most differently expressed genes between LF/LCL and SF/SCL in the ampulla (n = 5) and the isthmus (n = 6).

<p>The colors in the map display the relative standing of the reads count data; GREEN indicates a count value that is lower than the mean value of the row while red indicates higher than the mean. The shades of the color indicate distance from each data point to the mean value of the row. Columns represent individual samples of LF/LCL and SF/SCL.</p

Localization of Progesterone receptor (PGR) and Estradiol receptor alpha (Er alpha) in the bovine oviduct by immunohistochemistry.

<p>Representative images of PGR and ER alpha immunohistochemical localization in the ampulla and the isthmus of LF/LCL and SF/SCL groups at Day 4 of the estrous cycle (n = 5 per group). Original magnification: 20x (scale bar: 100 μm) and 60x (scale bar: 25 μm). For an overview of PGR and ER alpha staining on samples of all animals see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0145321#pone.0145321.s001" target="_blank">S1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0145321#pone.0145321.s002" target="_blank">S2</a> Figs.</p