Comparison of fully typed clinical isolates using ECDCT and SBMT.

<p>Only samples with fully-typed genotypes in both typing schemes are shown.</p

Flow chart of 54 patients included in the study.

<p>Flow chart of 54 patients included in the study.</p

Genotypes identified using SBMT to date in the Czech Republic and in Argentina [this study, 14, 15] and their phylogenetic and network analyses.

<p><b>A.</b> Genotypes identified exclusively in the Czech Republic (dark grey background), in Argentina (light grey background) and in both locations. Both fully and partially typed genotypes are shown. <b>B.</b> The phylogenetic tree of concatenated sequences of TP0136, TP0548, and 23S rRNA. Partially typed genotypes (XU8R9, XU3R8, XU4R9, and U5XS) were excluded. The U2SS genotype is also not shown due to the short sequence obtained from the TP0136 gene. Genotypes identified in this study are shown in bold. The bar scale represents 0.005 substitutions per target site. The numbers at each node correspond to bootstrap values. <b>C.</b> The network tree of concatenated sequences of TP0136, TP0548, and 23S rRNA genes. Partially typed genotypes (XU8R9, XU3R8, XU4R9 and U5XS) were excluded. The U2SS genotype is also not shown due to the short sequence obtained from the TP0136 gene. Every circle represents a different genotype. The small numbers represent the phylogenetic distances between genotypes. The black circle represents an unknown ancestor. Genotypes identified in the Czech Republic and in Argentina are shown in dark and light grey, respectively.</p

Characteristics of detected genotypes using SBMT on 41 samples from syphilis PCR-positive patients from Buenos Aires, Argentina.

<p>Characteristics of detected genotypes using SBMT on 41 samples from syphilis PCR-positive patients from Buenos Aires, Argentina.</p

Alignment of TP0136 unique sequences identified to date [this study, 14, 15, 19].

<p>Only positions containing nucleotide variants are shown. <b>A.</b> Nucleotide differences between reference strain TPA SS14 and TPA Nichols in the region characterized using SBMT (at coordinates 264–1469 according to the TPANIC_0136; CP004010.2). Coordinates shown above correspond to TPASS_0136 (TPA SS14; CP004011.1) and coordinates shown below correspond to TPANIC_0136 (TPA Nichols; CP004010.2). <b>B.</b> Nucleotide differences in SS14-like unique sequences. Coordinates correspond to TPASS_0136 (TPA SS14; CP004011.1). ND, was not determined due to the limited amount of sample DNA. The white lines between coordinates indicate the non-continuous nucleotide positions. <b>C.</b> Nucleotide differences in Nichols-like unique sequences. Coordinates correspond to TPANIC_0136 (TPA Nichols; CP004010.2). The new unique sequence identified in this study is shown in bold. The white lines between coordinates indicate the non-continuous nucleotide positions.</p

Alignment of all TP0548 unique sequences identified to date [this study, 14, 15, 19].

<p>Only positions containing nucleotide variants are shown. <b>A.</b> Nucleotide differences between reference strain TPA SS14 and TPA Nichols in the region characterized using SBMT (at coordinates 16–1080 of TPANIC_0548; CP004010.2). Coordinates shown above correspond to TPASS_0548 (TPA SS14; CP004011.1) and coordinates shown below correspond to TPANIC_0548 (TPA Nichols; CP004010.2). <b>B.</b> Nucleotide differences in SS14-like unique sequences. Coordinates correspond to TPASS_0548 (TPA SS14; CP004011.1). New unique sequences identified in this study are shown in bold. The white lines between coordinates indicate the non-continuous nucleotide positions. Highlighted coordinates shown in white (154, 155, 158) correspond to regions determined by ECDCT. <b>C.</b> Nucleotide differences in Nichols-like unique sequences. Coordinates correspond to TPANIC_0548 (TPA Nichols; CP004010.2). New unique sequence identified in this study is shown in bold. The white lines between coordinates indicate the non-continuous nucleotide positions. Highlighted coordinates shown in white (170, 208) correspond to regions determined by ECDCT.</p