Quantitative HPLC estimation of flavonoids in showy tick trefoil herbs

Quantitative and qualitative analysis of flavonoids in showy tick trefoil (Desmodium canadense L., Fabaceae family) herbs collected in various phases of vegetation at the Department of Medicinal Herbs of the Kaunas Botanical Garden (Vytautas Magnus University) was performed using HPLC techniques. A total of fifteen flavonoids of the aglicone and glycoside nature were identified, including apigenin, apigenin-7-O-glycoside, luteolin, rutin, vicenin-2, vitexin, isovitexin, vitexin rhamnoside, orientin, homoorientin, quercitrin, quercetin, hyperoside, astragalin, and kaempferol. The maximum amount of identified flavonoids (13573.83 mg/g) was observed at the budding stage. Among these flavonoids, orientin and homoorientin (6236.24 μg/g), vicenin-2 (2040.25 μg/g), vitexin (1717.12 μg/g), isovitexin (1767.82 μg/g) and rutin (925.72 μg/g) predominatedBotanikos sodasKauno medicinos universitetasKauno medicinos universitetas, [email protected] Didžiojo universiteta