Micropropagation of Ocotea porosa (Nees & Martius) Barroso

The objective of this work was the establishment of a micropropagation protocol for Ocotea porosa by multiplication of shoots from axillary buds. Different concentration of BAP (0; 2.5; 5 or 10 ìM) or BAP+KIN (0; 1.25; 2.5 or 5 ìM) were investigated to optimize the multiplication. Shoot growth was stimulated with reduced concentration of BAP (0; 0.5; 1 or 1.5 ìM) or KIN (0.5 or 1ìM) or activated charcoal (0.5; 1; 2 or 3 gl-1). For root induction different concentrations of IBA (0; 1.25; 2.5; 5 or 10 ìM) or (0; 2.5; 5 or 10 mM) were applied. The highest mean multiplication rate was observed in the fourth subculture with 5 ìM BAP, reaching 5.3 shoots per explant. The shoots elongated in culture medium supplemented with 2 gl-1 activated charcoal and presented bigger leaves than on medium with reduced concentration of BAP. The shoots rooted on medium contains 10 ìM IBA or after pulse treatment of 10 mM (68.7 and 62.6% of rooting, respectively). The survival rate of the plants was 56.7%. This study showed that O. porosa micropropagation is feasible; however it needs further research in order to increase plant survival.Key words: 6-Benzylaminopurine, multiplication, rooting, in vitro culture, apical shoots, native specie

Efficient micropropagation of Epidendrum secundum Jacq. from leaves and protocorms

An efficient method for the large-scale propagation of Epidendrum secundum using protocorms and leaves from in vitro germination was established. Explants were inoculated in Woody Plant Medium (WPM) supplemented with 6-benzyladenine (BA) (1.0–40.0 µM) for 60 days and cultured in a growth room in darkness (30 days) or with a 16 h photoperiod. Protocorms were most effective for regenerating protocorm-like bodies (PLBs; 100.0%), independent of the presence or absence of light. The leaves were less responsive, and the best results were obtained with the youngest leaves (58.0% PLB induction). BA influenced the responses of protocorms and young leaves, and medium supplemented with 1.0 µM BA was most effective for inducing and regenerating PLBs. The shoots were rooted (100.0%) in WPM without any growth regulator and were transplanted to Isopor trays containing Plantmax florestal® and vermiculite (2:1), with 100.0% survival after 60 days. A histological analysis demonstrated that for both the explants, leaves and protocorms, PLBs were formed from successive divisions of epidermal cells. This simple protocol will be useful for the large-scale propagation of E. secundum.Key words: Orchidaceae, epiphytic orchid, in vitro propagation, micropropagation.Abbreviation: BA, 6-Benzyladenine; PLBs, protocorm-like bodies; WPM, woody plant medium