9 research outputs found
Non-chemical signalling between mitochondria
A wide variety of studies have reported some form of non-chemical or non-aqueous communication between physically isolated organisms, eliciting changes in cellular proliferation, morphology, and/or metabolism. The sources and mechanisms of such signalling pathways are still unknown, but have been postulated to involve vibration, volatile transmission, or light through the phenomenon of ultraweak photon emission. Here, we report non-chemical communication between isolated mitochondria from MCF7 (cancer) and MCF10A (non-cancer) cell lines. We found that mitochondria in one cuvette stressed by an electron transport chain inhibitor, antimycin, alters the respiration of mitochondria in an adjacent, but chemically and physically separate cuvette, significantly decreasing the rate of oxygen consumption compared to a control (p = <0.0001 in MCF7 and MCF10A mitochondria). Moreover, the changes in O2-consumption were dependent on the origin of mitochondria (cancer vs. non-cancer) as well as the presence of āambientā light. Our results support the existence of non-chemical signalling between isolated mitochondria. The experimental design suggests that the non-chemical communication is light-based, although further work is needed to fully elucidate its nature
Ultra weak photon emissionāa brief review
Cells emit light at ultra-low intensities: photons which are produced as by-products of cellular metabolism, distinct from other light emission processes such as delayed luminescence, bioluminescence, and chemiluminescence. The phenomenon is known by a large range of names, including, but not limited to, biophotons, biological autoluminescence, metabolic photon emission and ultraweak photon emission (UPE), the latter of which shall be used for the purposes of this review. It is worth noting that the photons when produced are neither āweakā nor specifically biological in characteristics. Research of UPE has a long yet tattered past, historically hamstrung by a lack of technology sensitive enough to detect it. Today, as technology progresses rapidly, it is becoming easier to detect and image these photons, as well as to describe their function. In this brief review we will examine the history of UPE research, their proposed mechanism, possible biological role, the detection of the phenomenon, and the potential medical applications
Rooting out ultraweak photon emission a-mung bean sprouts
It is well known that life has evolved to use and generate light, for instance, photosynthesis, vision and bioluminescence. What is less well known is that during normal metabolism, it can generate 1-100 photons sā1 cm-2 known as ultra-weak photon emission (UPE), biophoton emission or biological autoluminescence. The highest generation of these metabolic photons seem to occur during oxidative stress due to the generation and decay of reactive oxygen species (ROS), and their interaction with other components of the cell. To study this further, we have configured a sensitive detection system to study photon emission in germinating mung beans.
Here we investigated growing mung beans over 7 days at a constant temperature of 21 Ā± 1Ā°C in a light tight box, using dual top and bottom opposing photomultiplier tubes. Over this time period we showed that in total, mung beans grown from seeds generated an average of 5 Ā± 1 counts sā1 above background. As the new bean stems grew, they showed a gradual linear increase in emission of up to 30 Ā± 1 counts sā1, in agreement with previous literature. In addition to this āsteady-stateā emission we also observe delayed luminescence and drought-stress response emission previously observed in other species. Finally, we also observe episodic increased emission events of between 2 and 15 counts sā1 for durations of around 3 hours detected underneath the sample, and assign these to the growing of secondary roots.
We then induce secondary root formation using aqueous solutions of growth hormones hydrogen peroxide (H2O2, 167 ĀµM) or 3-indole acetic acid (IAA, 0.5 ĀµM) for watering. Both hormones show prolonged increase in emission above steady-state, over days 3-5 with at least 3 times the number of secondary roots formed compared with water alone. We also observed a significant peak increase in photon emission (474 and 1738 cps vs. 28 and 55 cps for water alone) for the H2O2 which we attribute to direct ROS reaction emission as confirmed by measurement on dead plants.
Altogether we have expanded upon and demonstrated an instrument and biological system for reliably producing and measuring intrinsic metabolic photons, first observed 100 years ago by Alexander Gurwitsch
Preprint: Near infrared-light treatment alters mitochondrial homeostasis to induce senescence in breast cancer cells
The application of near infrared (NIR)-light to living systems has been suggested as a potential method to enhance tissue repair, decrease inflammation, and possibly mitigate cancer therapy-associated side effects. In this study, we examined the effect of exposing three cell lines: breast cancer (MCF7), non-cancer breast cells (MCF10A), and lung fibroblasts (IMR-90), to 734 nm NIR-light for 20 minutes per day for six days, and measuring changes in cellular senescence. Positive senescent populations were induced using doxorubicin. Flow cytometry was used to assess relative levels of senescence together with mitochondria-related variables. Exposure to NIR-light significantly increased the level of senescence in MCF7 cells (13.5%; P<0.01), with no observable effects on MCF10A or IMR-90 cell lines. NIR-induced senescence was associated with significant changes in mitochondria homeostasis, including raised ROS level (36.0%; P<0.05) and mitochondrial membrane potential (14.9%; P<0.05), with no changes in mitochondrial Ca2+. These results suggest that NIR-light exposure can significantly arrest the proliferation of breast cancer cells via inducing senescence, while leaving non-cancerous cell lines unaffected
Rooting out ultraweak photon emission a-mung bean sprouts
It is well known that life has evolved to use and generate light, for instance, photosynthesis, vision and bioluminescence. What is less well known is that during normal metabolism, it can generate 1ā100Ā photonsĀ sā1Ā cmā2 known as ultra-weak photon emission (UPE), biophoton emission or biological autoluminescence. The highest generation of these metabolic photons seem to occur during oxidative stress due to the generation and decay of reactive oxygen species (ROS), and their interaction with other components of the cell. To study this further, we have configured a sensitive detection system to study photon emission in germinating mung beans.Here we investigated growing mung beans over 7 days at a constant temperature of 21Ā Ā±Ā 1Ā Ā°C in a light tight box, using dual top and bottom opposing photomultiplier tubes. Over this time period we showed that in total, mung beans grown from seeds generated an average of 5Ā Ā±Ā 1Ā countsĀ sā1 above background. As the new bean stems grew, they showed a gradual linear increase in emission of up to 30Ā Ā±Ā 1Ā countsĀ sā1, in agreement with previous literature. In addition to this āsteady-stateā emission we also observe delayed luminescence and drought-stress response emission previously observed in other species. Finally, we also observe episodic increased emission events of between 2 and 15Ā countsĀ sā1 for durations of around 3Ā h detected underneath the sample, and assign these to the growing of secondary roots.We then induce secondary root formation using aqueous solutions of growth hormones hydrogen peroxide (H2O2, 167Ā ĀµM) or 3-indole acetic acid (IAA, 0.5Ā ĀµM) for watering. Both hormones show prolonged increase in emission above steady-state, over days 3ā5 with at least 3 times the number of secondary roots formed compared with water alone. We also observed a significant peak increase in photon emission (474 and 1738 cps vs. 28 and 55 cps for water alone) for the H2O2 which we attribute to direct ROS reaction emission as confirmed by measurement on dead plants.Altogether we have expanded upon and demonstrated an instrument and biological system for reliably producing and measuring intrinsic metabolic photons, first observed 100 years ago by Alexander Gurwitsch
Image1_Non-chemical signalling between mitochondria.tif
A wide variety of studies have reported some form of non-chemical or non-aqueous communication between physically isolated organisms, eliciting changes in cellular proliferation, morphology, and/or metabolism. The sources and mechanisms of such signalling pathways are still unknown, but have been postulated to involve vibration, volatile transmission, or light through the phenomenon of ultraweak photon emission. Here, we report non-chemical communication between isolated mitochondria from MCF7 (cancer) and MCF10A (non-cancer) cell lines. We found that mitochondria in one cuvette stressed by an electron transport chain inhibitor, antimycin, alters the respiration of mitochondria in an adjacent, but chemically and physically separate cuvette, significantly decreasing the rate of oxygen consumption compared to a control (p = 2-consumption were dependent on the origin of mitochondria (cancer vs. non-cancer) as well as the presence of āambientā light. Our results support the existence of non-chemical signalling between isolated mitochondria. The experimental design suggests that the non-chemical communication is light-based, although further work is needed to fully elucidate its nature.</p
Image4_Non-chemical signalling between mitochondria.tif
A wide variety of studies have reported some form of non-chemical or non-aqueous communication between physically isolated organisms, eliciting changes in cellular proliferation, morphology, and/or metabolism. The sources and mechanisms of such signalling pathways are still unknown, but have been postulated to involve vibration, volatile transmission, or light through the phenomenon of ultraweak photon emission. Here, we report non-chemical communication between isolated mitochondria from MCF7 (cancer) and MCF10A (non-cancer) cell lines. We found that mitochondria in one cuvette stressed by an electron transport chain inhibitor, antimycin, alters the respiration of mitochondria in an adjacent, but chemically and physically separate cuvette, significantly decreasing the rate of oxygen consumption compared to a control (p = 2-consumption were dependent on the origin of mitochondria (cancer vs. non-cancer) as well as the presence of āambientā light. Our results support the existence of non-chemical signalling between isolated mitochondria. The experimental design suggests that the non-chemical communication is light-based, although further work is needed to fully elucidate its nature.</p
Image2_Non-chemical signalling between mitochondria.tif
A wide variety of studies have reported some form of non-chemical or non-aqueous communication between physically isolated organisms, eliciting changes in cellular proliferation, morphology, and/or metabolism. The sources and mechanisms of such signalling pathways are still unknown, but have been postulated to involve vibration, volatile transmission, or light through the phenomenon of ultraweak photon emission. Here, we report non-chemical communication between isolated mitochondria from MCF7 (cancer) and MCF10A (non-cancer) cell lines. We found that mitochondria in one cuvette stressed by an electron transport chain inhibitor, antimycin, alters the respiration of mitochondria in an adjacent, but chemically and physically separate cuvette, significantly decreasing the rate of oxygen consumption compared to a control (p = 2-consumption were dependent on the origin of mitochondria (cancer vs. non-cancer) as well as the presence of āambientā light. Our results support the existence of non-chemical signalling between isolated mitochondria. The experimental design suggests that the non-chemical communication is light-based, although further work is needed to fully elucidate its nature.</p
Image3_Non-chemical signalling between mitochondria.tif
A wide variety of studies have reported some form of non-chemical or non-aqueous communication between physically isolated organisms, eliciting changes in cellular proliferation, morphology, and/or metabolism. The sources and mechanisms of such signalling pathways are still unknown, but have been postulated to involve vibration, volatile transmission, or light through the phenomenon of ultraweak photon emission. Here, we report non-chemical communication between isolated mitochondria from MCF7 (cancer) and MCF10A (non-cancer) cell lines. We found that mitochondria in one cuvette stressed by an electron transport chain inhibitor, antimycin, alters the respiration of mitochondria in an adjacent, but chemically and physically separate cuvette, significantly decreasing the rate of oxygen consumption compared to a control (p = 2-consumption were dependent on the origin of mitochondria (cancer vs. non-cancer) as well as the presence of āambientā light. Our results support the existence of non-chemical signalling between isolated mitochondria. The experimental design suggests that the non-chemical communication is light-based, although further work is needed to fully elucidate its nature.</p