Pulmonary paracoccidioidomycosis in immunized mice

Paracoccidioidomycosis was induced in immunized (IM) and non-immunized (NI) mice. The histopathology, the number of fungi in the lungs, the cellular (footpad test - FPT and macrophage inhibition factor assay - MIF) and humoral (immunodiffusion test) immune response were investigated serially postinfection. In the IM mice, at days 1 and 3, there was intense and predominant macrophagic-lymphocytic alveolitis with loose granulomatous reaction; at day 30, inflammation was mild. In the NI group, up to day 3, the lesions were focal; later there was formation of extensive epithelioid granuloma. The number of fungi in IM mice were always smaller than those of NI group. Immunization alone induced positive FPT and MIF indices with low titer of antibody. After infection, there was a significant decrease of the FPT indices in the IM group, which we interpreted as desensitization due to trapping of sensitized lymphocytes in the lungs. In conclusion, (1) The lesional pattern of pulmonary paracoccidioidomycosis in IM mice was similar to that of a hypersensitivity pneumonitis. This reaction was probably effective in reducing the extension of the infection and decrease the number of fungi. (2) In this model, pulmonary resistance against P. brasiliensis seems to be related to local and systemic delayed-type hypersensitivity reaction. © 1992 Kluwer Academic Publishers

INVIVO and INVITRO EVALUATION of CELL-MEDIATED-IMMUNITY in PATIENTS WITH PARACOCCIDIOIDOMYCOSIS

ESCOLA PAULISTA MED,DEPT MICROBIOL IMUNOL & PARASITOL,São Paulo,BRASILUNIV São Paulo,FAC MED CLIN HOSP,DEPT CLIN MED,SECAO ALERGIA,São Paulo,BRASILESCOLA PAULISTA MED,DEPT MICROBIOL IMUNOL & PARASITOL,São Paulo,BRASILWeb of Scienc

Humoral and cell-mediated immunity in large non-toxic multinodular goitre

Humoral and cell-mediated immunity was investigated in fourteen patients with non-toxic multinodular goitre and ten healthy controls by in vitro methods. These included determination of sheep erythrocyte and complement rosette-forming cells in the peripheral blood, immunoglobulin levels, titres of thyroglobulin and microsomal antibodies and migration inhibition test using thyroid extract and phytohemagglutinin. When compared with controls the patients showed high IgA levels and positive response to thyroid antigen in the leucocyte migration inhibition test. There was no correlation between the leucocyte migration results and the presence of auto-antibodies. These findings indicate a possible role of cell-mediated immunity in non-toxic multinodular goitre

Immunological disturbances in toxic multinodular goitre and active Graves' disease

Cell-mediated and humoral immunity were studied in seventeen patients with toxic multinodular goitre, ten with active Graves' disease and fourteen healthy controls. The study included determination of sheep erythrocyte and complement rosette-forming cells in peripheral blood, immunoglobulin levels, titres of microsomal antibodies and migration inhibition test using thyroid extract and phytohaemagglutinin. When compared with controls the patients showed a positive response to thyroid antigen in the leucocyte migration inhibition test. Microsomal antibodies were detected in seven out of ten active Graves' disease patients against two out of seventeen of those with toxic multinodular goitre. Significantly increased IgG and IgA and decreased IgM levels were found only in the toxic multinodular group. These data provide further evidence for immunological disturbances in toxic multinodular goitre

Investigation of the electronic and magnetic properties of Mn doped ZnO using the FP-LAPW method

We report on the results of the structural, electronic and magnetic properties of ZnO (1 x 1 x 1), (1 x 2 x 2) in the zincblende (ZB) and rocksalt (RS) phases produced by doping Mn in ZnO structures, considering, for the magnetic interaction between the Mn atoms, both the near and far positions. These are evaluated using density functional theory (DFT). The band gaps of the ZnO semiconductor are calculated by the full potential linearized augmented plane wave (FP-LAPW) method with the local spin density approximation (LSDA) and the modified Becke-Johson (mBJ) potential. The results of the theoretical calculations are compared to the experimental values. The gaps of RS-ZnO are 0.735(LDA) eV and 2.688(LDA+mBJ) eV. They are comparable to 2.45 eV; in the zincblende phase, the gaps are 0.694(LDA) eV and 2.946(LDA+mBJ) eV compared to the 3.27 eV experimental value. Both the band gap and the total magnetic moment of Mn doped ZnO increased in the supercell 1 x 2 x 2 for the RS and ZB phases. Our analysis revealed that the mBJ potential is very efficient for the determination of the band gaps of ZnO semiconductors; it is clear that the mBJ potential gives good results for the treatment of the d-orbitals. (C) 2017 The Physical Society of the Republic of China (Taiwan). Published by Elsevier B.V. All rights reserved.WOS:0004076130000392-s2.0-8502784796