39 research outputs found
In vitro regeneration ability of diploid and autotetraploid plants of Cichorium intybus L.
Polyploidy has played a significant role in the evolutionary history of plants and is a valuable tool for obtaining useful characteristics. Because of the novelty of polyploids, comparison of their in vitro culture responses with diploids would be notable. In this study, leaf explants from diploid, autotetraploid and mixoploid plants of Cichorium intybus L. were cultured in vitro on the similar media and under same conditions. The ploidy level of the obtained calluses and regenerants were determined by flow cytometry analysis. The callogenic response of leaf explants cultured on the callus induction medium did not depend on the ploidy level of their parental plants. According to the flow cytometry analysis, the increased ploidy levels (4x) and (8x) were observed in the callus cultures with diploid and tetraploid origin, respectively. A considerable difference was observed between the ploidy level of mixoploid plants and their calluses, indicating the dominance of diploid cells in the callus tissue. The results showed that polyploidy led to the loss of organogenic potential as the tetraploid origin calluses failed to regenerate, while the diploid origin calluses successfully regenerated to whole plants.ΠΠΎΠ»ΠΈΠΏΠ»ΠΎΠΈΠ΄ΠΈΡ ΡΡΠ³ΡΠ°Π»Π° Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΡΡ ΡΠΎΠ»Ρ Π² ΡΠ²ΠΎΠ»ΡΡΠΈΠΎΠ½Π½ΠΎΠΉ ΠΈΡΡΠΎΡΠΈΠΈ ΡΠ°ΡΡΠ΅Π½ΠΈΠΉ ΠΊΠ°ΠΊ ΡΠ΅Π½Π½ΡΠΉ ΠΈΠ½ΡΡΡΡΠΌΠ΅Π½Ρ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ ΠΏΠΎΠ»Π΅Π·Π½ΡΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ². Π Π½Π°ΡΡΠΎΡΡΠ΅ΠΉ ΡΠ°Π±ΠΎΡΠ΅ ΠΏΡΠΈΠ²Π΅Π΄Π΅Π½ΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΠ΅ ΠΊΡΠ»ΡΡΡΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΎΡΠ²Π΅ΡΠ° in vitro ΠΏΠΎΠ»ΠΈΠΏΠ»ΠΎΠΈΠ΄ΠΎΠ² Ρ Π΄ΠΈΠΏΠ»ΠΎΠΈΠ΄Π°ΠΌΠΈ. ΠΠΈΡΡΠΎΠ²ΡΠ΅ ΡΠΊΡΠΏΠ»Π°Π½ΡΡ Π΄ΠΈΠΏΠ»ΠΎΠΈΠ΄Π½ΡΡ
, Π°Π²ΡΠΎΡΠ΅ΡΡΠ°ΠΏΠ»ΠΎΠΈΠ΄Π½ΡΡ
ΠΈ ΠΌΠΈΠΊΡΠΎΠΏΠ»ΠΎΠΈΠ΄Π½ΡΡ
ΡΠ°ΡΡΠ΅Π½ΠΈΠΉ Cichorium intybus L. ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π»ΠΈ Π½Π° ΠΎΠ΄Π½ΠΈΡ
ΠΈ ΡΠ΅Ρ
ΠΆΠ΅ ΡΡΠ΅Π΄Π°Ρ
Π² ΠΎΠ΄ΠΈΠ½Π°ΠΊΠΎΠ²ΡΡ
ΡΡΠ»ΠΎΠ²ΠΈΡΡ
. Π£ΡΠΎΠ²Π΅Π½Ρ ΠΏΠ»ΠΎΠΈΠ΄Π½ΠΎΡΡΠΈ ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΠΊΠ°Π»Π»ΡΡΠΎΠ² ΠΈ ΡΠ΅Π³Π΅Π½Π΅ΡΠ°Π½ΡΠΎΠ² ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΆΠΈΠ΄ΠΊΠΎΡΡΠ½ΠΎΠΉ ΡΠΈΡΠΎΠΌΠ΅ΡΡΠΈΠΈ. Π‘ΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡ Π»ΠΈΡΡΠΎΠ²ΡΡ
ΡΠΊΡΠΏΠ»Π°Π½ΡΠΎΠ² ΠΊ ΠΊΠ°Π»Π»ΡΡΠΎΠΎΠ±ΡΠ°-Π·ΠΎΠ²Π°Π½ΠΈΡ Π½Π° ΡΡΠ΅Π΄Π΅ Π΄Π»Ρ ΠΈΠ½Π΄ΡΠΊΡΠΈΠΈ ΠΊΠ°Π»Π»ΡΡΠ° Π½Π΅ Π·Π°Π²ΠΈΡΠ΅Π»Π° ΠΎΡ ΡΡΠΎΠ²Π½Ρ ΠΏΠ»ΠΎΠΈΠ΄Π½ΠΎΡΡΠΈ ΠΈΡΡ
ΠΎΠ΄Π½ΡΡ
ΡΠ°ΡΡΠ΅Π½ΠΈΠΉ. ΠΠΎ Π΄Π°Π½Π½ΡΠΌ ΠΆΠΈΠ΄ΠΊΠΎΡΡΠ½ΠΎΠΉ ΡΠΈΡΠΎΠΌΠ΅ΡΡΠΈΠΈ Π² ΠΊΠ°Π»Π»ΡΡΠ½ΡΡ
ΠΊΡΠ»ΡΡΡΡΠ°Ρ
Π΄ΠΈΠΏΠ»ΠΎΠΈΠ΄Π½ΠΎΠ³ΠΎ ΠΈ ΡΠ΅ΡΡΠ°ΠΏΠ»ΠΎΠΈΠ΄Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΡ Π½Π° Π±Π»ΡΠ΄Π°Π»ΠΎΡΡ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΡΡΠΎΠ²Π½Π΅ΠΉ ΠΏΠ»ΠΎΠΈΠ΄Π½ΠΎΡΡΠΈ (4x ΠΈ 8x ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ). ΠΠ½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΡΠ΅ ΠΎΡΠ»ΠΈΡΠΈΡ Π² ΡΡΠΎΠ²Π½ΡΡ
ΠΏΠ»ΠΎΠΈΠ΄Π½ΠΎΡΡΠΈ ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½Ρ Ρ ΠΌΠΈΠΊΡΠΎΠΏΠ»ΠΎΠΈΠ΄Π½ΡΡ
ΡΠ°ΡΡΠ΅Π½ΠΈΠΉ ΠΈ ΠΈΡ
ΠΊΠ°Π»Π»ΡΡΠΎΠ², ΠΏΡΠΈ ΡΡΠΎΠΌ Π² ΠΊΠ°Π»Π»ΡΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ Π΄ΠΎΠΌΠΈΠ½ΠΈΡΠΎΠ²Π°Π»ΠΈ Π΄ΠΈΠΏΠ»ΠΎΠΈΠ΄Π½ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΏΠΎΠ»ΠΈ ΠΏΠ»ΠΎΠΈΠ΄ΠΈΡ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΠ»Π° ΠΊ ΠΏΠΎΡΠ΅ΡΠ΅ ΠΎΡΠ³Π°Π½ΠΎΠ³Π΅Π½Π½ΠΎΠ³ΠΎ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»Π°, ΡΠ°ΠΊ ΠΊΠ°ΠΊ ΡΠ΅ΡΡΠ°ΠΏΠ»ΠΎΠΈΠ΄Π½ΡΠ΅ ΠΊΠ°Π»Π»ΡΡΡ Π½Π΅ Π±ΡΠ»ΠΈ ΡΠΏΠΎΡΠΎΠ±Π½Ρ ΠΊ ΡΠ΅Π³Π΅Π½Π΅ΡΠ°ΡΠΈΠΈ, Π° Π² ΠΊΠ°Π»Π»ΡΡΠ°Ρ
Π΄ΠΈΠΏΠ»ΠΎΠΈΠ΄Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΡ ΡΡΠΏΠ΅ΡΠ½ΠΎ ΠΏΡΠΎΠΈΡΡ
ΠΎΠ΄ΠΈΠ»Π° ΡΠ΅Π³Π΅Π½Π΅ΡΠ°ΡΠΈΡ ΡΠ°ΡΡΠ΅Π½ΠΈΠΉ
Adaptive morpho-anatomical characteristics of leaves and cones in Juniperus seravschanica Kom.: Studies of polyphenolic parenchyma cells, secretory tissues, sclereids and tracheids
Tungstate ion (WO<sub>4</sub><sup>2-</sup>) confined in hydrophilic/hydrophobic nanomaterials functionalized brΓΆnsted acidic ionic liquid as highly active catalyst in the selective aerobic oxidation of alcohols in water
Oxidative Stress Tolerance by Calcium and Histidine in Two Tomato Cultivars Under Nickel Stress
We investigated calcium (Ca) and L-histidine (His) interaction on nickel (Ni)-induced oxidative stress tolerance in two tomato (Solanum lycopersicum Mill.) cultivars including Cal-J N3 and Petoearly CH. CaCl2 (0 and 300 Β΅M) and L-histidine (0 and 300 Β΅M) effects on the oxidative responses in these cultivars cultured were compared in the hydroponic media under Ni stress (NiSO4; 0,150 and 300 Β΅M). The activities of antioxidative enzymes including catalase (CAT), guaiacol peroxidase (GPX), ascorbate peroxidase (APX), superoxide dismutase (SOD) and total content of proteins, malondialdehyde (MDA), other aldehydes, H2O2, Ca2+, Ni2+, ascorbate (ASC), dehydroascorbate (DHA) and electrolytes leakage (EL) were determined. The obtained results indicated that the application of Ca and His generally reduced oxidative markers such as the contents of EL, H2O2, MDA and activity of CAT as well as the Ni2+content of root and shoot organs under nickel toxicity, while application of Ni treatment without Ca+His increased these oxidative parameters and accumulation of Ni2+, compared to the control. Applying Ni without Ca and His has resulted in reduction of GPX, APX and SOD activities as well as concentrations of root and shoot Ca2+and ASC in the two mentioned cultivars. Application of Ca and His lead to the elevated contents of Ca2+ and ASC, increased activities of GPX, APX and SOD as well as inhibition of Ni2+ accumulation differently in both cultivars. Ca and His also alleviated the adverse effects of Ni stress on the selected investigated parameters especially in Petoearly CH cultivar. Thus, interaction of Ca and His appeared to improve adaptive responses to Ni stress leading to decreasing Ni-induced oxidative stress in the tomato plants. Therefore, our results suggest that Ca+His alleviated nickel-induced oxidative stress by uptake and inhibition of translocation of Ni2+ plus Ni chelating mechanism improvement in the tomato cultivars
Tungstate ion (WO42-) confined in hydrophilic/hydrophobic nanomaterials functionalized brΓΆnsted acidic ionic liquid as highly active catalyst in the selective aerobic oxidation of alcohols in water
A BrΓΆnsted acidic Ionic Liquid containing tungstate anion functionalized polysiloxane network (PMO-IL-WO42-) was synthesized by simple self-condensation of tungstic acid and zwitterionic organosilane precursor possessing both imidazolium and sulfonate groups. Characterization by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), X-ray powder diffraction (XRD), thermal gravimetric analysis TGA, nitrogen porosimetry, solid-state NMR spectroscopy and elemental analysis confirmed that both imidazolium cation and tungstate anion of zwitterion are successfully incorporated inside the organosilica framework. The catalytic activity of resulting hybrid PMO-IL- WO42- material was studied in the selective aerobic oxidation of primary and secondary alcohols using an atmospheric pressure of air in pure water. Due to the ionic liquid-based charged surface containing hydrophilic sulfonic acid and tungstate group, the synergistic hydrophilic/hydrophobic and redox effect of PMO-IL-WO42- as water-friendly catalyst facilitates and enhances the activity and selectivity toward the target oxidative products in water and proved to have a particularly broad substrate scope for reliable aerobic oxidation reaction. Furthermore, the catalyst showed outstanding stability and could be easily separated and reused at least ten reactions run under the same conditions as fresh catalyst without any loss of catalytic activity and product selectivity. Β© 202
Optimization of wave energy converters in the OPWEC project
International Conference on Maritime Technology and Engineering (MARTECH) (4th, 2018, Lisbon, Portugal