DILG Negros Occidental Contact Tracer Information Management System

COVID-19 pandemic reshapes the use of technology and innovation in an organization. Organizations are adopting and utilizing technologies in their day-to-day operations. Information Systems offer a wide range of solutions in terms of data management and interconnecting people with the use of a system. Its use was proven to enhance and improve the productivity of an organization in this pandemic. These promising results led the researcher to develop the Department of the Interior and Local Government (DILG) Negros Occidental Contact Tracer Information Management System (CTIMS). The CTIMS is a web application that enables DILG Negros Occidental Personnel to manage and facilitate all DILG Hired-Contact Tracers (CT) in the Province of Negros Occidental. The automated system made the data collection easy and error-free, streamlined reporting, and eliminated the time-consuming process of consolidating the Accomplishment Reports (ARs) from the Field and Provincial Offices. The document tracker feature replaced the manual entry in the logbook. It also improved the received and released document reporting. It improved the processes of the CT Task team compared to the manual operations and is expected to increase the productivity, effectivity, and efficiency of DILG Negros Occidental.&nbsp

Efficient secretion of the model antigen M6-gp41E in Lactobacillus plantarum NCIMB 8826.

Four Lactobacillus strains (Lb. plantarum NCIMB 8826, Lb. paracasei LbTGS1.4, Lb. casei ATCC 393 and Lb. fermentum KLD) were tested for their ability to produce and secrete heterologous proteins. These strains were first screened with an alpha-amylase reporter under the control of a set of expression or expression/secretion signals from various lactic acid bacteria. With most of the constructions tested, the level of extracellular production was highest in Lb. plantarum NCIMB 8826, and lowest in Lb. paracasei LbTGS1.4. These two strains were next assayed using a model antigen consisting of the N-terminal part of the M6 protein from Streptococcus pyogenes fused to the linear epitope ELDKWAS from human immunodeficiency virus gp41 protein. Secretion of this heterologous protein was inefficient in Lb. paracasei LbTGS1.4, which accumulated a large intracellular pool of the unprocessed precursor, whereas Lb. plantarum NCIMB 8826 was able to secrete the antigen to a level as high as 10 mg l-1

Production of cholera toxin B subunit in Lactobacillus

The intracellular expression of the B subunit of cholera toxin (CTB) was first achieved in Lactobacillus paracasei LbTGS1.4 with an expression cassette including the P25 promoter of Streptococcus thermophilus combined with the translation initiation region from the strongly expressed L. pentosus D-lactate dehydrogenase gene (ldhD). Secretion of CTB was next attempted in L. paracasei LbTGS1.4 and L. plantarum NCIMB8826 with four different signal sequences from exported proteins of lactic acid bacteria (Lactococcus lactis Usp45 and PrtP, Enterococcus faecalis unknown protein and S. pyogenes M6 protein). Host-dependent secretion of CTB was clearly observed: whereas none of the secretion cassettes led to detectable CTB in the extracellular fraction of L. paracasei LbTGS1.4, secretion of CTB molecules was clearly achieved with three of the selected signal sequences in L. plantarum NCIMB8826. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved

Final measurement. Deliverable D5.3

This deliverable D5.3 presents the GEN6 Final Measurement. It describes the outputs, outcomes and impact of the GEN6 project, based on other project deliverables inputs of the pilot leaders of the active GEN6 pilots and the individual consortium partners. The final measurement aims to show the amount of achievements within GEN6 and spill-over of pilot experiences to other organisation: (“paving the way for others”), in order to addresses the main goal the European Commission has stated for this project. It also presents the main attention points for IPv6 introduction experienced in seven different IPv6-pilots