6 research outputs found
Computer Simulation of Cytoskeleton-Induced Blebbing in Lipid Membranes
Blebs are balloon-shaped membrane protrusions that form during many
physiological processes. Using computer simulation of a particle-based model
for self-assembled lipid bilayers coupled to an elastic meshwork, we
investigated the phase behavior and kinetics of blebbing. We found that blebs
form for large values of the ratio between the areas of the bilayer and the
cytoskeleton. We also found that blebbing can be induced when the cytoskeleton
is subject to a localized ablation or a uniform compression. The results
obtained are qualitatively in agreement with the experimental evidence and the
model opens up the possibility to study the kinetics of bleb formation in
detail.Comment: To appear in Physical Review
Tethered Particle Motion Reveals that LacI·DNA Loops Coexist with a Competitor-Resistant but Apparently Unlooped Conformation
AbstractThe lac repressor protein (LacI) efficiently represses transcription of the lac operon in Escherichia coli by binding to two distant operator sites on the bacterial DNA and causing the intervening DNA to form a loop. We employed single-molecule tethered particle motion to observe LacI-mediated loop formation and breakdown in DNA constructs that incorporate optimized operator binding sites and intrinsic curvature favorable to loop formation. Previous bulk competition assays indirectly measured the loop lifetimes in these optimized DNA constructs as being on the order of days; however, we measured these same lifetimes to be on the order of minutes for both looped and unlooped states. In a range of single-molecule DNA competition experiments, we found that the resistance of the LacI-DNA complex to competitive binding is a function of both the operator strength and the interoperator sequence. To explain these findings, we present what we believe to be a new kinetic model of loop formation and DNA competition. In this proposed new model, we hypothesize a new unlooped state in which the unbound DNA-binding domain of the LacI protein interacts nonspecifically with nonoperator DNA adjacent to the operator site at which the second LacI DNA-binding domain is bound
Flanking DNA Matters in DNA Loop Formation and Breakdown Mediated by Lac Repressor Protein
DNA Competition Experiments Reveal the Importance of Operator Binding Strength and Inter-Operator Sequence in Protein-Mediated DNA Looping
Implicit-solvent mesoscale model based on soft-core potentials for self-assembled lipid membranes
An efficient implicit-solvent model for self-assembled lipid bilayers is presented and analyzed using Langevin molecular dynamics simulations. The model is based on soft interactions between particles and short-range attractive interaction between lipid tails, leading for the self-assembly of a lipid bilayer without an explicit solvent. This allows for efficient simulations of large membranes over long times. The model exhibits a fluid phase at high temperatures and a gel phase at low temperatures, identified with the Lβ-phase. The melting transition is investigated via analysis of the diffusivity of the lipid molecules, the chain-orientational order parameter, the sixfold bond-orientational order parameter and the positional and bond-orientational correlation functions. The analysis suggests the existence of a hexatic phase over a narrow range of temperatures around the melting transition. The elastic properties of the membrane in the fluid phase are also investigated