The combination of bortezomib and resveratrol may prevent muscle wasting in diabetes

Hypercatabolic syndrome is a biochemical state characterized by a imbalance between catabolism and anabolism in favor of catabolism. Diabetes is an example of hypercatabolic syndrome with presence of decreased insulin level or impaired insulin signaling besides increased inflammatory cytokines. One of the significant outcomes of this state is accelerated protein degradation and muscle wasting. Increased ubiquitin-proteasomal system activity is the major responsible for the muscle wasting. Increase in expression and activities of proteasomal proteins in diabetes had been determined. NF-kappa B transcription factor mediated inflammation and oxidative stress accompanies proteasomal activity increase. Oxidative stress continuously produces substrate for proteasomes by causing protein oxidation. An intervention that inhibits proteasomal activity, suppressing inflammation and oxidative stress may form a solution in order to prevent muscle wasting. Therefore, I am considering that the combined use of bortezomib, a proteasome inhibitor and an anti-inflammatory with resveratrol, an antioxidant and anti-inflammatory, could prevent diabetes induced muscle wasting. This combination may be a novel therapeutic approach for muscle wasting. (C) 2010 Elsevier Ltd. All rights reserved

The Analytical Performance of a Microplate Method for Total Sulfhydryl Measurement in Biological Samples

Objective: Sulfhydryl groups of protein, glutathione and free cysteine are readily oxidized to disulfides by oxidants, and are important indicators of oxidative stress. Our aim was to apply the Ellman method for assay of sulfhydryl groups to microplate scale and to determine this method's analytical performance