Comparative internal and external anatomy of <i>Tyrannobdella rex.</i>

<p>(A) Whole body ventral view illustrating annulation, relative size of the caudal sucker and relative position of gonopores. (B) Eyespot arrangement illustrated dorsally. (C) Male and female median reproductive anatomy.</p

Mucosally invasive hirudinoid leeches.

<p>Known from a wide variety of anatomical sites including eyes (A) as in this case involving <i>Dinobdella ferox</i> (B), mucosal leech species, as in a case involving <i>Myxobdella annandalei</i> (C), more frequently feed from the nasopharyngeal surfaces of mammals (D).</p

Single most parsimonious tree based on combined 18S rDNA, 28s rDNA, 12s rDNA, and COI datasets.

<p>The family Praobdellidae formed a well-supported monophyletic group of leeches that exhibits a predilection for mammalian mucosa. All groups received 100 percent bootstrap support and posterior probabilities of 1.00 except as noted on the tree. Branches are drawn proportional to amount of change.</p

Genes and primer sequences used in phylogenetic analyses.

<p>Genes and primer sequences used in phylogenetic analyses.</p

Taxa used for the phylogenetic analyses, collection localities, and GenBank accession numbers.

<p>Type species of genera are indicated with an asterisk.</p

Comparative jaw morphology of <i>Tyrannobdella rex</i>.

<p>(A) Stereomicrograph of the single dorsal jaw of <i>T. rex</i> with large teeth. Scale bar is 100 µm. (B) <i>Tyrannobdella rex</i> anterior sucker exhibiting velar mouth and longitudinal slit through which the dorsal jaw protrudes when feeding. Scale bar is 1 mm. (C) Compound micrograph in lateral view of eight large teeth of <i>T. rex</i>. Scale bar is 100 µm. (D) Lateral view of jaw of <i>Limnatis paluda</i> illustrating typical size of hirudinoid teeth. Scale bar is 100 µm.</p