Feasibility Study on Soilless Cultivation of Organic Ginseng

Based on the present situation and problems concerning ginseng cultivation as well as soilless cultivation features, we analyze the growth indicators and input-output ratio of different ginseng cultivation patterns, and conform that the soilless cultivation technology for organic ginseng is feasible. And this technology provides theoretical basis and technological feasibility for the sustainable development of ginseng industry

Changes in Soil Nutrients of Farmland with Different Cultivation Years of Panax ginseng

Through analyzing the soil organic matters (N, P, K) of farmland cultivated with different years of Panax ginseng, this paper studied the changes in soil nutrients of farmland with different vertical depths and cultivation years of P. ginseng. Results indicated that the vertical structure was obvious in soil nutrients of farmland with different cultivation years of P. ginseng; in most cases, the soil nutrient content gradually declined with the fibrous roots of P. ginseng spreading downward; the soil electrical conductivity (EC), total nitrogen (TN), total phosphorus (TP), total potassium (TK), available nitrogen, available phosphorus were manifested as surface layer > root layer > bottom layer, while the available potassium was manifested as surface soil and bottom layer > root layer; the soil pH changed in the range of 5.69-6.22, suitable for growth of P. ginseng. It is expected to provide theoretical basis for improvement of soil nutrients of farmland with cultivation of P. ginseng

Effects of Biogas Slurry Recirculation on Anaerobic Digestion Performance of Maize Straw Silage

In order to investigate the effects of slurry recirculation technology on anaerobic digestion performance of maize straw silage, maize straw silage was fermented with recirculated biogas slurry, and the gas production, pH value, methane content, volatile organic acids (VFAs) contents, chemical oxygen demand (COD) removal rate and other indicators were studied. The results showed that the fermentation time was positively correlated with daily gas production, methane content, cumulative gas production, VFAs and COD removal rate. Although the pH value fluctuated, it was still in the normal reaction range. The daily gas production was about 1.26 L. The acetic acid content increased first, then decreased, then increased, and finally stabilized. The biogas slurry recirculation technology saves water resources by 40 mL/d without affecting the normal gas production of anaerobic fermentation, and reduces the consumption of environmental resources. It has important development significance for the sustainable use of biomass resources

Effects of Agitating Intensity on Anaerobic Digestion Performance of Corn Straw Silage

Anaerobic fermentation can increase biomass energy use efficiency of crop straws and realizes win-win of energy and environment. This paper explored the biogas generation performance of anaerobic digestion of cow dung liquid as nitrogen source in three different levels of stirring intensity at 30℃ constant temperature condition. Through pH value, biogas production, chemical oxygen demand (COD), methane content, volatile fatty acid (VFA), principal component analysis (PCA) and modified Gompertz model, effects of agitating intensity on anaerobic digestion performance of corn straw silage were evaluated. Results indicate that the COD removal rate of three agitating intensity levels is higher than 85%, and pH value is about 6.5; the cumulative biogas production after 20 days is 2 h ＞ 4 h ＞ 1 h of agitating; in the 49th day, the biogas production is 1.9 L at 30 min / 2 h, 1.7 L at 30 min / 4h, and 1.6 L at 30 min / h; the maximum biogas production rate is 30 min / 2 h ＞ 30 min / 4 h＞30 min / h; and the maximum methane production rate is 30 min / 4 h ＞ 30 min / 2 h ＞ 30 min / h; in the same energy consumption, the biogas production at 30 min / 4h is higher than 1 h. In conclusion, overall analysis of energy consumption and economic factors indicate that 30 min/ 4 h agitating intensity is more suitable for straw biogas fermentation project. This study is expected to provide theoretical foundation for biogas fermentation project

Effect of Spent Mushroom Substrate on Physical and Chemical Properties and Enzymic Activity of Rice

In order to explore the substitution substrate for rice seedling on upland fields, this paper uses spent mushroom substrate to study the physical and chemical properties of substrate, enzymic activity and number of tillers during the cultivation of rice seedling on upland fields. The results show that at the three stages of rice seedling cultivation (two-leaf stage, three-leaf stage, four-leaf stage), the content of organic matter and EC in spent mushroom substrate is higher than in the control soil, pH is within the range suitable for the growth of rice, and other nutrients (total nitrogen, total phosphorus, total potassium, available nitrogen, available phosphorus) are slightly different in different periods; except phosphatase, there are significant differences in urease, catalase and sucrase between spent mushroom substrate and the control soil; the number of tillers under spent mushroom substrate is larger than under the control

Antagonistic Effects of Sphingomonas and Pseudomonas aeruginosa on 4 Kinds of Pathogenic Bacteria of Ginseng

[Objectives] To explore effective biocontrol methods for diseases in the process of ginseng cultivation, and develop an efficient and environmentally friendly biocontrol agent. [Methods] In this study, 2 strains were isolated from biogas slurry, and Cylindrocarpon destructans (XF), Fusarium solani (GF), Botrytis cinerea Pers (HM) and Alternaria panax Whetz (HB) were used as test materials. The strains were isolated and identified by dilution plate method, 16S rDNA sequence identification method, confrontation culture method, filter paper method and ultraviolet spectrophotometer method, and the bacteriostatic activity and bacteriostatic rate were tested. [Results] Strain 15 (Sphingomonas) and strain 19 (Pseudomonas aeruginosa) were screened out through identification and analysis, and they grew stably within 8-10 d. The bacteriostatic rates of strain 15 against A. panax and B. cinerea were 47.37% and 43.40%, respectively, and the bacteriostatic rates of strain 19 against A. panax and B. cinerea were 62.30% and 63.27%, respectively. The bacteriostatic activity of the extract of strain 19 increased with the increase of OD600 value, and the bacteriostatic effect was optimal when the OD600 value was in the range of 0.8-1, up to about 70%, so it had a strong biocontrol potential. [Conclusions] This experiment provides convenience for more effective inoculation, establishes a fast, simple and accurate method for the determination of the best bacteriostatic rate of P. aeruginosa culture solution to HM, and lays a foundation for large-scale culture of P. aeruginosa culture solution. Besides, it is expected to provide a theoretical basis for the efficient control of ginseng B. cinerea in field production, use it for the prevention and control of ginseng shoot diseases, and provide a reference for the efficient and diverse development of biocontrol agents for ginseng shoot diseases

Degradation of lignocelluloses in straw using AC-1, a thermophilic composite microbial system

In composting, the degradation of lignocellulose in straw is problematic due to its complex structures such as lignin. A common solution to this problem is the addition of exogenous inoculants. AC-1, a stable thermophilic microbial composite, was isolated from high temperature compost samples that can decompose lignocellulose at 50–70 °C. AC-1 had a best degradation efficiency of rice straw at 60 °C (78.92%), of hemicellulose, cellulose and lignin were 82.49%, 97.20% and 20.12%, respectively. It showed degrad-ability on both simple (filter paper, absorbent cotton) and complex (rice straw) cellulose materials. It produced acetic and formic acid during decomposition process and the pH had a trend of first downward then upward. High throughput sequencing revealed the main bacterial components of AC-1 were Tepidimicrobium, Haloplasma, norank-f-Limnochordaceae, Ruminiclostridium and Rhodothermus which provides major theoretical basis for further application of AC-1

The Complete Chloroplast Genome Sequences of Fritillaria ussuriensis Maxim. and Fritillaria cirrhosa D. Don, and Comparative Analysis with Other Fritillaria Species

The genus Fritillaria belongs to the widely distributed Liliaceae. The bulbs of Fritillaria, F. ussuriensis and F. cirrhosa are valuable herbaceous medicinal ingredients. However, they are still used indiscriminately in herbal medicine. Identification and molecular phylogenic analysis of Fritillaria species are therefore required. Here, we report the complete chloroplast (CP) genome sequences of F. ussuriensis and F. cirrhosa. The two Fritillaria CP genomes were 151,524 and 151,083 bp in length, respectively, and each included a pair of inverted repeated regions (52,678 and 52,156 bp) that was separated by a large single copy region (81,732 and 81,390 bp), and a small single copy region (17,114 and 17,537 bp). A total of 111 genes in F. ussuriensis and 112 in F. cirrhosa comprised 77 protein-coding regions in F. ussuriensis and 78 in F. cirrhosa, 30 transfer RNA (tRNA) genes, and four ribosomal RNA (rRNA) genes. The gene order, content, and orientation of the two Fritillaria CP genomes exhibited the general structure of flowering plants, and were similar to those of other Fritillaria species. Comparison of the six Fritillaria species’ CP genomes indicated seven highly divergent regions in intergenic spacers and in the matK, rpoC1, rpoC2, ycf1, ycf2, ndhD, and ndhF coding regions. We established the position of the six species through phylogenic analysis. The complete chloroplast genome sequences of the two Fritillaria species and a comparison study are useful genomic information for identifying and for studying the phylogenetic relationship among Fritillaria species within the Liliaceae

Meta-Analysis of Factors Affecting C-N Fractions and Yield of Paddy Soils by Total Straw Return and N Fertilizer Application

The effective use of nutrient-rich crop straw is an important way to use resources efficiently and to sustain agricultural development. This meta-analysis study collected and analyzed the data of 6788 observations published in 238 peer-reviewed papers to investigate differences in soil C-N fractions and yields of paddy soils under different straw-return amounts. This large dataset was also used to quantify the degree of influence of factors such as climate characteristics, soil properties, N fertilizer application rates, straw-rotting agent addition, rice varieties, and straw return methods. The results showed that straw return amounts improved soil alkaline-hydrolysable N (7%), total N (10%), organic C (11%), the C:N ratio (8%), rice N accumulation (12%), and overall yield (18%). The most significant effect was in northeast China fields for total soil nitrogen (TN) content and yield with increases of 13% and 22%, respectively. We also found more effective N utilization and a greater rice yield when 220–260 kg ha−1 N fertilizer was applied with 20–30 kg ha−1 straw-rotting agent with the total amount of straw return. These findings have important implications for choosing appropriate conditions and field management practices and to improve rice yield in China

The Impacts on Spinach Growth and Yield by Biological Organic Fertilizer

To decrease fertilization amount of chemical fertilizer and improve the quality of vegetable crops, spinach was taken as the test material, and the impact of different fertilizer on spinach growth and yield was studied via the manners of biological organic fertilizer and organic fertilizer+chemical fertilizer. Experimental results showed that in the formula of organic fertilizer+chemical fertilizer, chlorophyll and nitrogen contents in spinach leaves obviously increased; in the formula of only adding organic fertilizer, spinach leaf temperature, leaf width, root length, plant height and fresh weight were all better than those in the formula of organic fertilizer+chemical fertilizer, and better formulas were A5, E5, F3 and I5, in which spinach plant height in E5 was 5.63 times higher than G5, root length in E5 was 2.67 times higher than G5, and fresh weight in G5 was 32.6 times higher than G5. By comprehensive analysis, the most suitable formula for spinach production was E5, and the research could provide theoretic basis for fertilization amount of organic fertilizer required by spinach growth and development