MOLECULAR IDENTIFICATION AND FACTORIAL OPTIMIZATION OF MICROBIAL ISOLATES FOR POLYOL PRODUCTION

ABSTRACT Xylitol is a pentitol, a natural sweetener, found its application in food/ pharmaceutical industries, exploited especially for its anti-cariogenic properties. Xylitol is a valuable product with rising demands in market, it becomes significant to study and optimize the production with more of economic justification. In this work, xylitol obtained by biotechnological methods was based on fermentation by yeast isolated from sugarcane extracts collected from the Sathyamangalam, Tamilnadu, India. Yeast isolates were identified using conventional microbiological approach, modern metagenomic analysis and the gene sequence was deposited in NCBI repository. The growth of Candida parapsilosis strain BKR1 (NCBI accession no.:KC462059) andits xylitol production capability were assessed. The single factorial optimization revealed that pH, Temperature and initial substrate concentration was 4, 30ºC and 0.1 g/ml respectively

Stability and rheological study on carbon-based nanofluids

In this study, an organic derived nanofluid has been developed from bio-origin resources. Carbon nanopowder (CNP) is obtained from derived rice husk and was prepared via a simple two-steps thermal process with minimum energy (low temperature and reaction time) using solar assisted plasma furnace. Nanofluids comprised of CNP and EG/water binary mixture has been prepared at various concentration such as 0.02 – 0.10 vol% of CNP. Flow curve of nanofluids showed that at minimum inclusion of CNP improved the stress of the fluid significantly. More to the addition, dynamic viscosity measure possesses that addition of CNP stabilized the properties of the fluid compared to virgin base fluid. Moreover, the stability results showed that the nanofluids stabilized starting from 1 week onwards as evidenced by UV-Visible spectrophotometer analysis. Furthermore, little to no precipitate noticed even after 8 weeks. This work offers greener approach for nanofluids which organic derived and environmentally friendly (very low percentage of nanoparticle, 0.02 vol%