ARTICLE Development of a Polishing Step Using a Hydrophobic Interaction Membrane Adsorber With a PER.C6 1 -Derived Recombinant Antibody

ABSTRACT: Membrane chromatography has already proven to be a powerful alternative to polishing columns in flowthrough mode for contaminant removal. As flow-through utilization has expanded, membrane chromatography applications have included the capturing of large molecules, including proteins such as IgGs. Such bind-and-elute applications imply the demand for high binding capacity and larger membrane surface areas as compared to flow-through applications. Given these considerations, a new Sartobind Phenyl TM membrane adsorber was developed for large-scale purification of biomolecules based on hydrophobic interaction chromatography (HIC) principles. The new hydrophobic membrane adsorber combines the advantages of membrane chromatography-virtually no diffusion limitation and shorter processing time-with high binding capacity for proteins comparable to that of conventional HIC resins as well as excellent resolution. Results from these studies confirmed the capability of HIC membrane adsorber to purify therapeutic proteins with high dynamic binding capacities in the range of 20 mg-MAb/cm 3 -membrane and excellent impurity reduction. In addition the HIC phenyl membrane adsorber can operate at five-to ten-fold lower residence time when compared to column chromatography. A bind/elute purification step using the HIC membrane adsorber was developed for a recombinant monoclonal antibody produced using the PER.C6 1 cell line. Loading and elution conditions were optimized using statistical design of experiments. Scaleup is further discussed, and the performance of the membrane adsorber is compared to a traditional HIC resin used in column chromatography

ARTEFACTS: How do we want to deal with the future of our one and only planet?

The European Commission’s Science and Knowledge Service, the Joint Research Centre (JRC), decided to try working hand-in-hand with leading European science centres and museums. Behind this decision was the idea that the JRC could better support EU Institutions in engaging with the European public. The fact that European Union policies are firmly based on scientific evidence is a strong message which the JRC is uniquely able to illustrate. Such a collaboration would not only provide a platform to explain the benefits of EU policies to our daily lives but also provide an opportunity for European citizens to engage by taking a more active part in the EU policy making process for the future. A PILOT PROGRAMME To test the idea, the JRC launched an experimental programme to work with science museums: a perfect partner for three compelling reasons. Firstly, they attract a large and growing number of visitors. Leading science museums in Europe have typically 500 000 visitors per year. Furthermore, they are based in large European cities and attract local visitors as well as tourists from across Europe and beyond. The second reason for working with museums is that they have mastered the art of how to communicate key elements of sophisticated arguments across to the public and making complex topics of public interest readily accessible. That is a high-value added skill and a crucial part of the valorisation of public-funded research, never to be underestimated. Finally museums are, at present, undergoing something of a renaissance. Museums today are vibrant environments offering new techniques and technologies to both inform and entertain, and attract visitors of all demographics.JRC.H.2-Knowledge Management Methodologies, Communities and Disseminatio

The James Webb Space Telescope

The James Webb Space Telescope (JWST) is a large (6.6m), cold (50K), infrared-optimized space observatory that will be launched early in the next decade. The observatory will have four instruments: a near-infrared camera, a near-infrared multi-object spectrograph, and a tunable filter imager will cover the wavelength range, 0.6 to 5.0 microns, while the mid-infrared instrument will do both imaging and spectroscopy from 5.0 to 29 microns. The JWST science goals are divided into four themes. The End of the Dark Ages: First Light and Reionization theme seeks to identify the first luminous sources to form and to determine the ionization history of the early universe. The Assembly of Galaxies theme seeks to determine how galaxies and the dark matter, gas, stars, metals, morphological structures, and active nuclei within them evolved from the epoch of reionization to the present day. The Birth of Stars and Protoplanetary Systems theme seeks to unravel the birth and early evolution of stars, from infall on to dust-enshrouded protostars to the genesis of planetary systems. The Planetary Systems and the Origins of Life theme seeks to determine the physical and chemical properties of planetary systems including our own, and investigate the potential for the origins of life in those systems. To enable these observations, JWST consists of a telescope, an instrument package, a spacecraft and a sunshield. The telescope consists of 18 beryllium segments, some of which are deployed. The segments will be brought into optical alignment on-orbit through a process of periodic wavefront sensing and control. The JWST operations plan is based on that used for previous space observatories, and the majority of JWST observing time will be allocated to the international astronomical community through annual peer-reviewed proposal opportunities.Comment: 96 pages, including 48 figures and 15 tables, accepted by Space Science Review

Survival of the fittest: the impact of fit between warehouse management structure and warehouse context on warehouse performance

Warehouse operations are vital for the success of a supply chain. This paper explores fit among warehouse management structure and the context in which the warehouse operates as an important driver of warehouse performance. Warehouse management structure has been operationalised as the extensiveness by which warehouse operations are planned and controlled, and the complexity of the decision rules used for optimisation of the operations. Warehouse performance is measured using data envelopment analysis (DEA). Hypotheses are developed and tested in a survey study among 111 distribution warehouses in the Netherlands and Belgium. Our results show that indeed warehouse management structure should be adapted to context. In order to obtain high warehouse performance more complex warehouse tasks require more and more complex decision rules, and warehouses with more unpredictable demand require fewer tactical plans. These results can help warehouse managers to structure their warehouse management contingent on the context in which the warehouse operates

Organizing warehouse management

Purpose ¿ The purpose of this paper is to investigate how warehouse management, understood as a cluster of planning and control decisions and procedures, is organized and driven by task complexity (TC) and market dynamics (MD). Design/methodology/approach ¿ A multi-variable conceptual model is developed based on the literature and tested among 215 warehouses using a survey. Findings ¿ The results suggest that TC and MD are the main drivers of warehouse management, measured by planning extensiveness (PE), decision rules complexity, and control sophistication. Differences between production and distribution warehouses are found with respect to the relationship between assortment changes and PE. Furthermore, TC appears to be a main driver of the specificity of the warehouse management (information) system (WMS). Research limitations/implications ¿ This paper is based on 215 warehouses in The Netherlands and Flanders (Belgium); future research may test the model on a different sample. More research should be conducted to further validate the measures of the core dimensions of warehouse management. Practical implications ¿ Different levels of TC and MD characterize warehouses. Such a characterization is a first step in determining generic warehouse functionalities and helping managers to decide on the best software for their warehouse operations. Originality/value ¿ The paper defines the core dimensions of warehouse management, makes them measurable, tests them and assesses how these drivers impact specificity of WMS. The paper shows that PE in production warehouses is driven by different variables than in distribution centers

Exchange design patterns for electronic intermediaries

Intermediaries have introduced electronic services with varying success. One of the problems an intermediary faces is deciding what kind of exchange service it should offer to its customers and suppliers. For example, should it only provide a catalogue or should it also enable customers to order products? Developing the right exchange design is a complex undertaking because of the many design options on the one hand and the interests of multiple actors to be considered on the other. This is far more difficult than simple prescriptions like ‘creating a win-win situation’ suggest. We address this problem by developing design patterns for the exchanges between customers, intermediary, and suppliers related to role, linkage, transparency, and ovelty choices. For developing these design patterns, we studied four distinct electronic intermediaries and dentified exchange design choices that require trade-offs relating to the interests of customers, intermediary, and suppliers. The exchange design patterns contribute to the development of design theory for electronic intermediaries by filling a gap between basic business models and detailed business process designs