Notes for the Rural Criminologist: Conducting Field Research with Rural Law Enforcement

Drawing primarily on research with law enforcement officers in rural East Texas, this research note explores the practical challenges of conducting qualitative research with rural police and provides tips for successfully overcoming the barriers that arise. Conducting qualitative research in a rural setting, especially with rural law enforcement agencies and officers, presents unique challenges. As with all rural investigations, defining ‘rural’ and identifying a target space to study is the first substantial hurdle. Once a rural community has been identified, the researcher will face issues related to the geographic distance or isolation of their chosen community that can affect their physical access to the research site and data. Traveling to and navigating rural spaces requires extensive preparation that may be easily overlooked if the researcher is accustomed to collecting data in and from urban cities and agencies. Additionally, and perhaps more significantly, challenges involving sociocultural access accompany rural research projects. Regarding law enforcement specifically, the intersection of the rural community’s culture, dense social networks, and an often-distinct occupational police subculture can either be advantageous, or present obstacles, to successful completion of research. Furthermore, the rural researcher must consider their physical and emotional safety when interviewing, engaging, or participating with first responders. Backup may be delayed due to the geographic expansiveness of an agency’s jurisdiction and the fact that few officers are patrolling at a given time, and treatment in the event of a tragic encounter may be limited due to the lack – sometimes simultaneously in quantity and quality – of healthcare facilities and providers

Introduction to Volume 7, Issue 2 (Special Issue)

No abstract available

Introduction to Volume 7, Issue 1 (Special Issue)

No abstract available.

Development of a Cyberinfrastructure for Assessment of the Lower Rio Grande Valley North and Central Watersheds Characteristics

Lower Laguna Madre (LLM) is designated as an impaired waterway for high concentrations of bacteria and low dissolved oxygen. The main freshwater sources to the LLM flow from the North and Central waterways which are composed of three main waterways: Hidalgo/Willacy Main Drain (HWMD), Raymondville Drain (RVD), and International Boundary & Water Commission North Floodway (IBWCNF) that are not fully characterized. The objective of this study is to perform a watershed characterization to determine the potential pollution sources of each watershed. The watershed characterization was achieved by developing a cyberinfrastructure, and it collects a wide inventory of data to identify which one of the three waterways has a major contribution to the LLM. Cyberinfrastructure development using the Geographic Information System (GIS) database helped to comprehend the major characteristics of each area contributing to the watershed supported by the analysis of the data collected. The watershed characterization process started with delineating the boundaries of each watershed. Then, geospatial and non-geospatial data were added to the cyberinfrastructure from numerous sources including point and nonpoint sources of pollution. Results showed that HWMD and IBWCNF watersheds were found to have a higher contribution to the water impairments to the LLM. HWMD and IBWCNF comprise the potential major sources of water quality impairments such as cultivated crops, urbanized areas, on-site sewage facilities, colonias, and wastewater effluents

Siderophore-Based Microbial Adaptations to Iron Scarcity Across the Eastern Pacific Ocean

Nearly all iron dissolved in the ocean is complexed by strong organic ligands of unknown composition. The effect of ligand composition on microbial iron acquisition is poorly understood, but amendment experiments using model ligands show they can facilitate or impede iron uptake depending on their identity. Here we show that siderophores, organic compounds synthesized by microbes to facilitate iron uptake, are a dynamic component of the marine ligand pool in the eastern tropical Pacific Ocean. Siderophore concentrations in iron-deficient waters averaged 9 pM, up to fivefold higher than in iron-rich coastal and nutrient-depleted oligotrophic waters, and were dominated by amphibactins, amphiphilic siderophores with cell membrane affinity. Phylogenetic analysis of amphibactin biosynthetic genes suggests that the ability to produce amphibactins has transferred horizontally across multiple Gammaproteobacteria, potentially driven by pressures to compete for iron. In coastal and oligotrophic regions of the eastern Pacific Ocean, amphibactins were replaced with lower concentrations (1-2 pM) of hydrophilic ferrioxamine siderophores. Our results suggest that organic ligand composition changes across the surface ocean in response to environmental pressures. Hydrophilic siderophores are predominantly found across regions of the ocean where iron is not expected to be the limiting nutrient for the microbial community at large. However, in regions with intense competition for iron, some microbes optimize iron acquisition by producing siderophores that minimize diffusive losses to the environment. These siderophores affect iron bioavailability and thus may be an important component of the marine iron cycle

Identification of pre-leukaemic haematopoietic stem cells in acute leukaemia.

In acute myeloid leukaemia (AML), the cell of origin, nature and biological consequences of initiating lesions, and order of subsequent mutations remain poorly understood, as AML is typically diagnosed without observation of a pre-leukaemic phase. Here, highly purified haematopoietic stem cells (HSCs), progenitor and mature cell fractions from the blood of AML patients were found to contain recurrent DNMT3A mutations (DNMT3A(mut)) at high allele frequency, but without coincident NPM1 mutations (NPM1c) present in AML blasts. DNMT3A(mut)-bearing HSCs showed a multilineage repopulation advantage over non-mutated HSCs in xenografts, establishing their identity as pre-leukaemic HSCs. Pre-leukaemic HSCs were found in remission samples, indicating that they survive chemotherapy. Therefore DNMT3A(mut) arises early in AML evolution, probably in HSCs, leading to a clonally expanded pool of pre-leukaemic HSCs from which AML evolves. Our findings provide a paradigm for the detection and treatment of pre-leukaemic clones before the acquisition of additional genetic lesions engenders greater therapeutic resistance

Envisioning the future: creating sustainable, healthy and resilient BioCities

Numerous challenges – from population increase to climate change – threaten the sustainable development of cities and call for a fundamental change of urban development and green-blue resource management. Urban forests are vital in this transition, as they provide various ecosystem services and allow to re-shape and re-think cities. Based on a Europe-wide community effort with diverse experts centered around urban forests and urban greening, we propose five key research fields to generate the knowledge required to unlock fundamental changes in urban development and green-blue resource management: circular bioeconomy, climate resilience, governance, social and human environment, and biodiversity. To support the design of greener, cooler, more inclusive and resilient cities, all these research fields require inter- and transdisciplinary collaboration, engaging stakeholders in transforming urban engagement and functioning. We summarise main inter-, trans- und multidisciplinary research paths for each field and the cross-cutting knowledge areas that can help to address the challenges many cities face (e.g., modelling and assessment of the urban microclimate). For transforming cities further knowledge is needed on e.g., urban innovation, transition, participation, and more. Finally, we address how the identified research gaps can be implemented (e.g., international coordinated research effort, interdisciplinary networks)