Graph Relation Aware Continual Learning

Continual graph learning (CGL) studies the problem of learning from an infinite stream of graph data, consolidating historical knowledge, and generalizing it to the future task. At once, only current graph data are available. Although some recent attempts have been made to handle this task, we still face two potential challenges: 1) most of existing works only manipulate on the intermediate graph embedding and ignore intrinsic properties of graphs. It is non-trivial to differentiate the transferred information across graphs. 2) recent attempts take a parameter-sharing policy to transfer knowledge across time steps or progressively expand new architecture given shifted graph distribution. Learning a single model could loss discriminative information for each graph task while the model expansion scheme suffers from high model complexity. In this paper, we point out that latent relations behind graph edges can be attributed as an invariant factor for the evolving graphs and the statistical information of latent relations evolves. Motivated by this, we design a relation-aware adaptive model, dubbed as RAM-CG, that consists of a relation-discovery modular to explore latent relations behind edges and a task-awareness masking classifier to accounts for the shifted. Extensive experiments show that RAM-CG provides significant 2.2%, 6.9% and 6.6% accuracy improvements over the state-of-the-art results on CitationNet, OGBN-arxiv and TWITCH dataset, respective

Effects of metastasis-associated in colon cancer 1 inhibition by small hairpin RNA on ovarian carcinoma OVCAR-3 cells

<p>Abstract</p> <p>Background</p> <p>Metastasis-associated in colon cancer 1 (MACC1) is demonstrated to be up-regulated in several types of cancer, and can serve as biomarker for cancer invasion and metastasis. To investigate the relations between MACC1 and biological processes of ovarian cancer, MACC1 specific small hairpin RNA (shRNA) expression plasmids were used to investigate the effects of MACC1 inhibition on ovarian carcinoma OVCAR-3 cells.</p> <p>Methods</p> <p>Expressions of MACC1 were detected in different ovarian tissues by immunohistochemistry. MACC1 specific shRNA expression plasmids were constructed and transfected into OVCAR-3 cells. Then, expressions of MACC1 were examined by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. Cell proliferation was observed by MTT and monoplast colony formation assay. Flow cytometry and TUNEL assay were used to measure cell apoptosis. Cell migration was assessed by wound healing and transwell migration assay. Matrigel invasion and xenograft model assay were performed to analyze the potential of cell invasion. Activities of Met, MEK1/2, ERK1/2, Akt, cyclinD1, caspase3 and MMP2 protein were measured by Western blot.</p> <p>Results</p> <p>Overexpressions of MACC1 were detected in ovarian cancer tissues. Expression of MACC1 in OVCAR-3 cells was significantly down-regulated by MACC1 specific small hairpin RNA. In OVCAR-3 cells, down-regulation of MACC1 resulted in significant inhibition of cell proliferation, migration and invasion, meanwhile obvious enhancement of apoptosis. As a consequence of MACC1 knockdown, expressions of Met, p-MEK1/2, p-ERK1/2, cyclinD1 and MMP2 protein decreased, level of cleaved capase3 was increased.</p> <p>Conclusions</p> <p>RNA interference (RNAi) against MACC1 could serve as a promising intervention strategy for gene therapy of ovarian carcinoma, and the antitumor effects of MACC1 knockdown might involve in the inhibition of HGF/Met and MEK/ERK pathways.</p

Rotavirus nonstructural protein 1 antagonizes innate immune response by interacting with retinoic acid inducible gene I

<p>Abstract</p> <p>Background</p> <p>The nonstructural protein 1 (NSP1) of rotavirus has been reported to block interferon (IFN) signaling by mediating proteasome-dependent degradation of IFN-regulatory factors (IRFs) and (or) the β-transducin repeat containing protein (β-TrCP). However, in addition to these targets, NSP1 may subvert innate immune responses via other mechanisms.</p> <p>Results</p> <p>The NSP1 of rotavirus OSU strain as well as the IRF3 binding domain truncated NSP1 of rotavirus SA11 strain are unable to degrade IRFs, but can still inhibit host IFN response, indicating that NSP1 may target alternative host factor(s) other than IRFs. Overexpression of NSP1 can block IFN-β promoter activation induced by the retinoic acid inducible gene I (RIG-I), but does not inhibit IFN-β activation induced by the mitochondrial antiviral-signaling protein (MAVS), indicating that NSP1 may target RIG-I. Immunoprecipitation experiments show that NSP1 interacts with RIG-I independent of IRF3 binding domain. In addition, NSP1 induces down-regulation of RIG-I in a proteasome-independent way.</p> <p>Conclusions</p> <p>Our findings demonstrate that inhibition of RIG-I mediated type I IFN responses by NSP1 may contribute to the immune evasion of rotavirus.</p

Parent–Child Cohesion, Basic Psychological Needs Satisfaction, and Emotional Adaptation in Left-Behind Children in China: An Indirect Effects Model

This study aimed to validate an indirect effects model of parent–child cohesion in emotional adaptation (i.e., loneliness and depression) via basic psychological needs satisfaction in Chinese left-behind children as well as the applicability of the model to both genders. A cross-sectional study was conducted and included 1,250 children aged between 9 and 12 years (635 left-behind children and 615 non-left-behind children) from rural primary schools. The results showed that: (1) relative to non-left-behind children, left-behind children exhibited significantly higher loneliness and depression scores and greater disadvantages involving father–child cohesion, mother–child cohesion, and psychological needs satisfaction. (2) Father– and mother–child cohesion were significantly negatively correlated with loneliness and depression and significantly positively correlated with psychological needs satisfaction in left-behind children. (3) Through structural equation modeling showed that psychological needs satisfaction partially mediated the relationship between parent–child cohesion and emotional outcomes in left-behind children. (4) Through multi-group analyses showed significant gender differences in structural weighting between parent–child cohesion and emotional adaptation, in that parent–child cohesion in left-behind boys was a stronger negative predictor of unfavorable emotional outcomes relative to that observed in left-behind girls, while psychological needs satisfaction in left-behind girls was a stronger negative predictor of unfavorable emotional outcomes relative to that observed in left-behind boys. The implications of these findings for interventions directed at Chinese left-behind children were discussed

Motion Kinematics Analysis of a Horse Inspired Terrain-Adaptive Unmanned Vehicle With Four Hydraulic Swing Arms

All terrain vehicles (ATV) perform tasks in unstructured environments where the advanced adaptive ability of rigid terrain has become a key factor. In this article, we propose a novel horse inspired all terrain eight-wheeled vehicle with four swing arms for transportation in the mountain battlefield. The mechanism structure and system configuration of the ATV are designed based on the horse leg kinematics analysis. In order to analyze the obstacle surmounting strategy of the ATV, the kinematics model and the center of gravity of the ATV are represented. A model reference adaptive control method is proposed for the hydraulic attitude control system. Then the model for obstacle surmounting is proposed for dynamics performance and geometric kinematics. Additionally, the simulation is executed in Adams to verify the analysis and strategy. Finally, the experiment is demonstrated for climbing a vertical wall, which is a challenging and typical terrain of the mountain battlefield

Magnetically-targetable outer-membrane vesicles for sonodynamic eradication of antibiotic-tolerant bacteria in bacterial meningitis

Treatment of acute bacterial meningitis is difficult due to the impermeability of the blood-brain barrier, greatly limiting the antibiotic concentrations that can be achieved in the brain. Escherichia coli grown in presence of iron-oxide magnetic nanoparticles secrete large amounts of magnetic outer-membrane vesicles (OMVs) in order to remove excess Fe from their cytoplasm. OMVs are fully biomimetic nanocarriers, but can be inflammatory. Here, non-inflammatory magnetic OMVs were prepared from an E. coli strain in which the synthesis of inflammatory lipid A acyltransferase was inhibited using CRISPR/Cas9 mediated gene knockout. OMVs were loaded with ceftriaxone (CRO) and meso-tetra-(4-carboxyphenyl)porphine (TCPP) and magnetically driven across the blood-brain barrier for sonodynamic treatment of bacterial meningitis. ROS-generation upon ultrasound application of CRO- and TCPP-loaded OMVs yielded similar ROS-generation as by TCPP in solution. In vitro, ROS-generation by CRO- and TCPP-loaded OMVs upon ultrasound application operated synergistically with CRO to kill a hard-to-kill, CRO-tolerant E. coli strain. In a mouse model of CRO-tolerant E. coli meningitis, CRO- and TCPP-loaded OMVs improved survival rates and clinical behavioral scores of infected mice after magnetic targeting and ultrasound application. Recurrence did not occur for at least two weeks after arresting treatment.</p