Root, mycorrhiza and earthworm interactions: their effects on soil structuring processes, plant and soil nutrient concentration and plant biomass

Earthworms, arbuscular mycorrhiza fungi (AMF) and roots are important components of the belowground part of terrestrial ecosystem. However, their interacting effects on soil properties and plant growth are still poorly understood. A compartmental experimental design was used in a climate chamber in order to investigate, without phosphorus (P) addition, the single and combined effects of earthworms (Allolobophora chlorotica), AMF (Glomus intraradices) and roots (Allium porrum) on soil structure, nutrient concentration and plant growth. In our experimental conditions, plant roots improved soil structure stability (at the level of macroaggregates) whereas earthworms decreased it. AMF had no effect on soil structure stability but increased P transfer from the soil to the plant and significantly increased plant biomass. Earthworms had no direct influence on P uptake or plant biomass, and the N/P ratio measured in the shoots indicated that P was limiting. Interactions between AMF and earthworms were also observed on total C and N content in the soil and on total root biomass. Their effects varied temporally and between the different soil compartments (bulk soil, rhizosphere and drilosphere). After comparison with other similar studies, we suggest that effects of earthworms and AMF on plant production may depend on the limiting factors in the soil, mainly N or P. Our experiment highlights the importance of measuring physical and chemical soil parameters when studying soil organism interactions and their influence on plant performanc

Coupling X-ray computed tomography and freeze-coring for the analysis of fine-grained low-cohesive soils

This paper presents the coupling of freeze-core sampling with X-ray CT scanning for the analysis of the soil structure of fine-grained, low-cohesive soils. We used a medical scanner to image the 3D soil structure of the frozen soil cores, providing X-ray CT data at a millimetric resolution over freeze-cores that are up to 62.5 cm long and 25 cm wide. The obtained data and the changes in gray level values could be successfully used to identify and characterize different soil units with distinctly different physical properties. Traditional measurements of soil bulk density, carbon and particle size analyses were conducted within each of the identified soil units. These observations were used to develop a 3D model of soil bulk density and organic matter distribution for five freeze-cores obtained at a restored floodplain in Switzerland. The millimetric X-ray CT scanning was applied to detect the impact of freeze-coring on the soil structural integrity. This allows identifying undisturbed zones, a critical precondition for any subsequent assessment of soil structure. The proposed coupling is thought to be applicable to a wide range of other low-cohesive soil types and has a large potential for applications in hydrogeology, biology or soil science

Effects of endogeic earthworms on the soil organic matter dynamics and the soil structure in urban and alluvial soil materials

Earthworms are considered as key actors of soil processes at different spatial and temporal scales and provide essential ecosystem services linked to climate regulation or primary production. However, little is known about their basic functional roles (e.g. organic matter decomposition, soil structuring processes) in perturbed systems such as urban or alluvial soils. Alluvial soils are characterized by regular physical perturbation through flooding and associated erosion/sedimentation processes which are rather similar to perturbations (e.g. temporal instability, spatial heterogeneity) affecting urban soils. Due to their close soil characteristics, we hypothesized that in both cases, soil functioning is similar with respect to soil fauna activity. Under controlled conditions, our objective was to investigate the effects of two endogeic earthworm species, Allolobophora chlorotica (pink morph) and Aporrectodea rosea (the two most abundant species found in the studied urban site), on soil organic matter (SOM) dynamics and soil structure (network of earthworm burrows) comparing an urban and an alluvial soil. We investigated the growth of individuals (weight gain and reproduction success) and assessed their effects on SOM decomposition (cumulative C–CO2 emission, nitrogen and phosphorus mineralization) and soil structure (macroporosity, total length and connectivity of segments) after one and three months of incubation. Our results showed higher growth of A. rosea in the alluvial soil compared to the urban soil. However, the total length of burrows, carbon and nitrogen mineralization were often higher in the urban soil especially when the two species were combined. This trend can be mainly explained by lower organic matter content found in the urban soil which may influence positively the burrowing activity and negatively the growth of earthworms. Endogeic earthworms appear a key feature of the soil functioning in the urban context through their roles on organic matter transformation, the formation and maintenance of the soil structure

Solar radiation explains litter degradation along alpine elevation gradients better than other climatic or edaphic parameters

Organic matter (OM) decomposition has been shown to vary across ecosystems, suggesting that variation in local ecological conditions influences this process. A better understanding of the ecological factors driving OM decomposition rates will allow to better predict the effect of ecosystem changes on the carbon cycle. While temperature and humidity have been put forward as the main drivers of OM decomposition, the concomitant role of other ecosystem properties, such as soil physicochemical properties, and local microbial communities, remains to be investigated within large-scale ecological gradients. To address this gap, we measured the decomposition of a standardized OM source – green tea and rooibos tea – across 24 sites spread within a full factorial design including elevation and exposition, and across two distinct bioclimatic regions in the Swiss Alps. By analyzing OM decomposition via 19 climatic, edaphic or soil microbial activity-related variables, which strongly varied across sites, we identified solar radiation as the primary source of variation of both green and rooibos teabags decomposition rate. This study thus highlights that while most variables, such as temperature or humidity, as well as soil microbial activity, do impact decomposition process, in combination with the measured pedo-climatic niche, solar radiation, very likely by means of indirect effects, best captures variation in OM degradation. For instance, high solar radiation might favor photodegradation, in turn speeding up the decomposition activity of the local microbial communities. Future work should thus disentangle the synergistic effects of the unique local microbial community and solar radiation on OM decomposition across different habitats

Carbon storage and soil organic matter stabilisation in near-natural, restored and embanked Swiss floodplains

Over recent decades, the number of floodplain restoration projects has increased worldwide. In Switzerland, several projects have been implemented to maintain or recreate ecological functions of floodplains. Despite this, little is known about the potential of floodplain soils to release and/or accumulate carbon. In alluvial soils, carbon storage is strongly influenced by fluvial dynamics, and therefore a better understanding of carbon fluxes and stocks in such settings is clearly needed. To evaluate the impact of river restoration on carbon storage in alluvial soils, we aimed to quantify and explain carbon storage and soil organic matter (SOM) stabilisation in the uppermost soil humic layer. Three floodplains were investigated showing each of them different levels of human disturbance: a near-natural section along the Rhine River, and both restored and embanked sections along the Thur River and Emme River. Carbon storage was determined by total organic carbon (TOC) stocks. SOM stabilisation was evaluated by considering the TOC content in different granulometric fractions (1000–2000 μm, 500–1000 μm, and 250–500 μm) and the macro-aggregate formation, i.e. the abundance of water-stable aggregates (WSA) and the mean weight diameter of macro-aggregates (MWD). Our results show that the carbon storage and SOM stabilisation parameters were all related to soil properties such as clay, silt and total iron contents of the upper humic layer. Within each floodplain, carbon storage and SOM stabilisation parameters differed according to soil profile groups, thus reflecting a soil gradient evolution from bare alluvium soils to more stabilised soils and a hydric functioning (soils with hydromorphic features). In addition, river restoration showed various impacts on carbon storage and SOM stabilisation parameters depending on the floodplains, with a significant difference between embanked and restored sections for the Emme floodplain and no difference for the Thur floodplain

Direct and indirect effects of urban gardening on aboveground and belowground diversity influencing soil multifunctionality

Urban gardens are popular green spaces that have the potential to provide essential ecosystem services, support human well-being, and at the same time foster biodiversity in cities. We investigated the impact of gardening activities on five soil functions and the relationship between plant (600 spp.) and soil fauna (earthworms: 18 spp., springtails: 39 spp.) in 85 urban gardens (170 sites) across the city of Zurich (Switzerland). Our results suggest that high plant diversity in gardens had a positive effect on soil fauna and soil multifunctionality, and that garden management intensity decreased plant diversity. Indices of biological activity in soil, such as organic and microbial carbon and bacterial abundance, showed a direct positive effect on soil multifunctionality. Soil moisture and disturbance, driven by watering and tilling, were the driving forces structuring plant and soil fauna communities. Plant indicator values proved useful to assess soil fauna community structure, even in anthropogenic plant assemblages. We conclude that to enhance soil functions, gardeners should increase plant diversity, and lower management intensity. Soil protective management practices, such as applying compost, mulch or avoiding soil tilling, should be included in urban green space planning to improve urban biodiversity and nature’s contribution to people

Impact of two root systems, earthworms and mycorrhizae on the physical properties of an unstable silt loam Luvisol and plant production

Background and aims: Soil organisms are known to engineer the soil physical properties, but their impact is difficult to assess and poorly documented. Shrinkage analysis has a good potential for such assessment. This study analyses the effects of mycorrhizae (Glomus intraradices), earthworms (Allolobophora chlorotica) and two plants, Allium porrum (leek) and Petunia hybrida (petunia), on the physical properties of an unstable loamy Luvisol, as well as the biological interactions between the soil organisms. Methods: In addition to soil organism biomass, shrinkage analysis and soil aggregate stability analysis were used to characterize the soil physical properties. Results: The soil aggregate stability, specific volume and structural pores volumes were increased with plant roots compared to control. The drilling effect of roots could not explain the pore volume increase, which was several orders of magnitude larger than the volume of the roots. Leek had larger impact on volumes while petunia mostly increased soil aggregate stability. Mycorrhizae increased the soil stability and the soil volume. Earthworms alone decreased the pore volumes at any pore size, and plant roots mitigated this. Conclusions: Our results highlight (1) the large impact of soil biota on soil physical properties, (2) that their separated effects can either combine or mitigate each other and (3) that the observed changes are varying in intensity according to soil type and plant typ