Comparaison des méthodes de dosages des anticorps antithyroïdiens

STRASBOURG ILLKIRCH-Pharmacie (672182101) / SudocSudocFranceF

Contribution à l'étude des macroprolactinémies

STRASBOURG ILLKIRCH-Pharmacie (672182101) / SudocSudocFranceF

Etude de la distribution isotypique des auto-anticorps

STRASBOURG ILLKIRCH-Pharmacie (672182101) / SudocSTRASBOURG-Sc. et Techniques (674822102) / SudocSudocFranceF

LES MARQUEURS URINAIRES DU CANCER DE LA VESSIE

STRASBOURG ILLKIRCH-Pharmacie (672182101) / SudocSudocFranceF

Combined search for anti–β2-glycoprotein i and anticardiolipin antibodies in antiphospholipid syndrome: Contribution to diagnosis

International audienc

Heterogeneous clinical spectrum of anti-SRP myositis and importance of the methods of detection of anti-SRP autoantibodies: a multicentric study

International audienceAnti-signal recognition particle (SRP) antibodies are important serological markers for the diagnosis and the prognosis of idiopathic inflammatory myopathy (IIM), especially to distinguish immune-mediated necrotizing myopathy (IMNM). This study was set up to investigate the phenotype associated with anti-SRP antibodies and to evaluate the methods for detecting these antibodies. Clinical and biological data were retrospectively obtained from 60 adult patients with anti-SRP antibodies detected by a dot immunoassay from 12 centers. Thirty-six (60 %) out of these 60 patients suffered from an IIM, and among them, 21 patients were diagnosed as IMNM. Among patients with a definite IIM, proximal weakness and myalgia were prominent symptoms at the time of diagnosis. Only few patients displayed severe extra-muscular symptoms such as cardiac involvement or severe myositis. Mean creatine kinase levels were high for all patients except for two of them. When testing by indirect immunofluorescence (IIF) on HEp2 cells, the fraction of patients displaying the typical anti-SRP fine speckled staining of the cytoplasm was higher in patients with IIM (30/36) (83 %) than in patients with non-IIM (3/24) (12.5 %) (p < 0.0001). Thirty (91 %) out of 33 patients with a positive immunodot and a characteristic IIF cytoplasmic staining suffered from a clinical definite myositis, whereas only 6 (22 %) out of 27 patients with a positive immunodot but a negative cytoplasmic pattern suffered from a myositis (p < 0.00001). This series highlights the strong heterogeneity of anti-SRP positivity that encompassed IMNM and non-IMNM and supports the necessity of considering both IIF and dot immunoassay to confirm the diagnosis of anti-SRP-associated myositis