A retrospective study of the clinical features of 50 consecutive cases diagnosed to have adenomyosis by histopathology in hysterectomy specimens in a tertiary centre

Background: Adenomyosis is characterised by the presence of ectopic endometrium, both glands and stroma deep in myometrium. Myometrial weakness caused by previous surgery or pregnancies, genetic factors, and tamoxifen use has been proposed as some aetiological factors.Methods: Present study aims to study the clinical profile of patients who have histological evidence of adenomyosis in hysterectomy specimens. The records of consecutive patients who had undergone hysterectomy were analysed.Results: The commonest age group affected is 41-50 years and menorrhagia is the predominant symptom.  The preoperative diagnosis of adenomyosis remains poor at around 8%.Conclusions: The age of onset and clinical features of patients should serve as an index for suspecting adenomyosis

तारली और मलबार सोल के स्टाँक पर बारिश का प्रभाव

कृपया पूरा लेखा पढ

Reentrant phase transitions of DNA-surfactant complexes

Complexes of double-stranded DNA with the cationic surfactant cetyltrimethylammonium bromide have been studied using small angle X-ray diffraction at varying concentrations of DNA and the cosurfactant hexanol. At low DNA concentrations, an intercalated hexagonal (HIc)→lamellar (Lαc)→inverted hexagonal (HIIc) transformation is found on increasing hexanol content. The HIIc structure is converted into Lαc on adding more DNA. Further increase in hexanol content leads to a phase separation in the surfactant solution, and a reentrant Lαc→HIIc→Lαc transition is observed as DNA concentration is increased. Such structural transformations of DNA-surfactant complexes, driven by DNA concentration, have not been reported until now

Structures of some surfactant-polyelectrolyte complexes

Structures of complexes formed in aqueous solutions by some anionic polyelectrolytes (double and single stranded (ds and ss) DNA, poly(vinyl sulfonate) (PVS), and poly(styrene sulfonate) (PSS)) with a cationic surfactant system consisting of cetyltrimethylammonium bromide (CTAB) and sodium 3-hydroxy-2-naphthoate (SHN) have been determined using small angle X-ray diffraction. All complexes are found to have a two-dimensional (2-D) hexagonal structure at low SHN concentrations. Analysis of the diffraction data shows that the ds DNA-CTAB complex has an intercalated structure, with each DNA strand surrounded by three cylindrical micelles. On increasing SHN concentration, DNA-CTAB-SHN complexes exhibit a hexagonal-to-lamellar transition, whereas PVS complexes show a hexagonal → centered rectangular → lamellar transition. PSS complexes show yet another sequence of structures. These results indicate the significant influence of the chemical nature of the polyelectrolyte on the structure of the complexes

Genetic diversity studies among AAB group Indian banana cultivars using ISSR markers

Banana and plantains are generally classified based on morphological characteristics namely AA, AAA, AB, AAB and ABB. Further, there are four sub-groups in AAB genomic group. Presently we analyzed diversity using ISSR markers in 18 cultivars of AAB genomic group along with two each of AA types and BB wild accessions for comparison. The results have shown that AAB cultivars form a separate group. Dendrogram analysis showed that the subgroups ‘Plantain’ ‘Silk’ and ‘Mysore’ were placed in between AA and BB type. Whereas ten cultivars of ‘Pome’ sub group of the cultivars were unique and was placed in a separate cluster. In this study using ISSR markers, we are able to identify the subgroups clearly and their genetic relationships within the AAB group. The cultivars Rasthali and Nendran were clearly separated. The Pome sub-group cultivars found to be in a group which may be based on their geographical origin

A note on a seed sterile nutmeg from the secondary center of domestication

A unique nutmeg accession having normal fruit, but with rudimentary, sterile seed and finely packed mace having a human brain like appearance was collected from a farmer’s garden from the secondary center of domestication of the crop and characterized. Seed (female) sterility in a dioecious or emerging monoecious plant like nutmeg is hitherto not recorded and is a novelty. This unique accession is conserved at the germplasm conservatory of tree spices at the ICAR-Indian Institute of Spices Research, Kozhikode, Kerala. &nbsp

Diversity in floral characters of monoecious nutmeg (Myristica fragrans Houtt.)

Floral diversity in monoecious type nutmeg reveals three types of flowers namely; pistillate, staminate and hermaphrodite flowers. The structure of hermaphrodite flowers in nutmeg is reported for the first time. The flowers are borne on the leaf axil, flowering habit of the three types of flowers are seen in cymes as well solitary in the same tree. The gynoecium consists of single ovary with bifid stigma in the pistillate flowers and the androecium of staminate flowers has adnate 7-13 anthers. Intra flower variability is evident in case of hermaphrodite flowers. In hermaphrodite flower the androecium ranges with 1-4 anthers, in the form of fused filament or free filament or both; some anthers are fused with the gynoecium. Besides remnants of the stamen in the developed fruits of hermaphrodite flowers, staminodes are also observed in the flowers. Colour of all the three types of flowers are light creamy yellow, with thick gamosepalous perianth which bursts as bilobed, trilobed, tetralobed and pentalobed lobes during anthesis. Analysis of variance of trees for flower types and floral attributes like flower type, number of anthers, length of anthers, length of filaments and length of ovary are found to be highly significant. High coefficient of variation is recorded for hermaphrodite (183.84) and pistillate (171.71%) flowers. The pollen viability of the hermaphrodite flowers are found less as compared to the staminate flowers. The percentage of pollen viability is 79.74% and 90.77% in hermaphrodite and male flower respectively. In the population studied, the occurrence of hermaphrodite flower in monoecious tree ranged from 0 - 10%

Manual on Entrepreneur-Ready Technologies of CMFRI

CMFRI presented five entrepreneur- ready technologies in the “Innovation 4 Industry Meet” organized by Zonal Technology Management Centre, Business Planning & Development Unit (ZTMCBPDU), South Zone, CIFT and National Fisheries Development Board held at Visakhapatnam on 8th September 2010 and in the ‘Rural Technology Mela’ organized by National Institute of rural Development at Hyderabad during 2nd to 5th February, 2011. A detailed presentation and exposition including prospective business proposals on the five technologies namely‘Green Mussel extract (GMe)’, ‘Varna (the marine ornamental fish feed)’, ‘Broodstock development, captive breeding and larval production of 17 species of marine ornamental fishes’, ‘Larval production of Cobia fish’ and ‘A device for breeding and culturing marine fish in open sea through cage farming’ were made in the meets. ‘Open sea cage farming in HDPE cage’ and ‘Low cost cage farming in GI cage’ were show-cased in the recently held ICAR-CII Industry Meet organized at NAAS Complex, New Delhi on 23rd May, 2011. The presentation and the exhibition in these meets got the attention of many prospective industrialists/ entrepreneurs which resulted in signing up of a few business proposals

Oil sardine from Oman enter fish markets of Kerala

The Oil sardine Sardinella longiceps is a major fishery resource in India and catches in 2014 were an estimated 5.44 lakh tonnes (t). Of this, Kerala accounted for 1.55 lakh t forming 28% of the All India Oil sardine landings. In the domestic retail markets of Kerala when Oil sardine prices were ranging between ` 80 - 140 per kilogram, large sized sardines with average weights > 120 g each were also appearing and being sold at prices 40 - 60% higher than the locally caught sardines. Although market sources informed that these large sized Oil sardine were coming from Oman, the claim could not be traced. However, during a weekly visit to Kalamukku landing centre, Kochi for sampling fish on 23.6.2015, the unloading of packed cartons containing large sized Oil sardine was observed and these were traced to being sourced from Oman

Isolation of protoplasts from cardamom (Elettaria cardamomum Maton.) and ginger (Zingiber officinale Rose.)

Protoplasts were isolated from leaf mesophyll tissue, collected from in vitro grown plantlets and cell suspension cultures of cardamom (Elettaria cardamomum) and ginger (Zingiber oJficinale). In cardamom, a protoplast yield of 3.5 x 105/ g of leaf tissue was obtained when incubated in an enzyme solution containing 0.5% macerozyme RIO, 2% cellulase Onozuka RIO and 9% mannitol for 18-20 h at 25°C in dark. The yield of protoplasts from cell suspension culture was 1.5 x 105 / g tissue, when incubated in I % macerozyme RIO and 2% cellulase Onozuka RIO for 24 h at 25°C with gentle shaking at 53 rpm in dark. The viability of leaf mesophyll protoplast was 75% and that of cell suspension was 40% on Evan's blue staining. In ginger, a protoplast yield of 2.5 x lOS / g of leaf tissue was obtained on digestion in an enzyme solution containing 0.5% macerozyme RIO, 3% hemicellulase and 5% cellulase Onozuka RIO, when incubated for 10 h at 15°C followed by 6 h at 30°C. The protoplast viability was 55%. Protoplast yield from cell suspension culture was 1 x lOS /g of callus when digested with an enzyme solution of I % macerozyme RIO, 3% hemicellulase and 6% cellulase Onozuka RIO and incubated for 10h at 15°C and later at 30°C for 8 h. Seventy two per cent of the protoplasts were viable. The protoplasts from both the species could be cultured and made to develop up to microcalli stage. &nbsp