923 research outputs found
Using participatory and creative methods to facilitate emancipatory research with people facing multiple disadvantage: a role for health and care professionals
Participatory and creative research methods are a powerful tool for enabling active engagement in the research process of marginalised people. It can be particularly hard for people living with multiple disadvantage, such as disabled people from ethnic minority backgrounds, to access research projects that are relevant to their lived experience. This article argues that creative and participatory methods facilitate the co-researchers’ engagement in the research process, which thus becomes more empowering. Exploring the congruence of these methods with their professional ethos, health and care professionals can use their skills to develop them further. Both theory and practice examples are presented
Influence of the substrate-induced strain and irradiation disorder on the Peierls transition in TTF-TCNQ microdomains
The influence of the combined effects of substrate-induced strain, finite
size and electron irradiation-induced defects have been studied on individual
micron-sized domains of the organic charge transfer compound
tetrathiafulvalene-tetracyanoquinodimethane (TTF-TCNQ) by temperature-dependent
conductivity and current-voltage measurements. The individual domains have been
isolated by focused ion beam etching and electrically contacted by focused ion
and electron beam induced deposition of metallic contacts. The
temperature-dependent conductivity follows a variable range hopping behavior
which shows a crossover of the exponent as the Peierls transition is
approached. The low temperature behavior is analyzed within the segmented rod
model of Fogler, Teber and Shklowskii, as originally developed for a
charge-ordered quasi one-dimensional electron crystal. The results are compared
with data obtained on as-grown and electron irradiated epitaxial TTF-TCNQ thin
films of the two-domain type
From biomolecules to biogeochemistry: Exploring the interaction of an indigenous bacterium with gold
Specialised microbial communities colonise the surface of gold particles in soils/sediments, and catalyse gold dissolution and re-precipitation, thereby contributing to the environmental mobility and toxicity of this ‘inert’ precious metal. We assessed the proteomic and physiological response of Serratia proteamaculans, the first metabolically active bacterium enriched and isolated directly from natural gold particles, when exposed to toxic levels of soluble Au3+ (10 μM). The results were compared to a metal-free blank, and to cultures exposed to similarly toxic levels of soluble Cu2+ (0.1 mM); Cu was chosen for comparison because it is closely associated with Au in nature due to similar geochemical properties. A total of 273 proteins were detected from the cells that experienced the oxidative effects of soluble Au, of which 139 (51%) were upregulated with either sole expression (31%) or had synthesis levels greater than the Au-free control (20%). The majority (54%) of upregulated proteins were functionally different from up-regulated proteins in the bacteria-copper treatment. These proteins were related to broad functions involving metabolism and biogenesis, followed by cellular process and signalling, indicating significant specificity for Au. This proteomic study revealed that the bacterium upregulates the synthesis of various proteins related to oxidative stress response (e.g., Monothiol-Glutaredoxin, Thiol Peroxidase, etc.) and cellular damage repair, which leads to the formation of metallic gold nanoparticles less toxic than ionic gold. Therefore, indigenous bacteria may mediate the toxicity of Au through two different yet simultaneous processes: i) repairing cellular components by replenishing damaged proteins and ii) neutralising reactive oxygen species (ROS) by up-regulating the synthesis of antioxidants. By connecting the fields of molecular bacteriology and environmental biogeochemistry, this study is the first step towards the development of biotechnologies based on indigenous bacteria applied to gold bio-recovery and bioremediation of contaminated environments.Santonu K. Sanyal, Tara Pukala, Parul Mittal, Frank Reith, Joël Brugger, Barbara Etschmann, Jeremiah Shuste
GRS 1915+105 : High-energy Insights with SPI/INTEGRAL
We report on results of two years of INTEGRAL/SPI monitoring of the Galactic
microquasar GRS 1915+105. From September 2004 to May 2006, the source has been
observed twenty times with long (approx 100 ks) exposures. We present an
analysis of the SPI data and focus on the description of the high-energy (> 20
keV) output of the source. We found that the 20 - 500 keV spectral emission of
GRS 1915+105 was bound between two states. It seems that these high-energy
states are not correlated with the temporal behavior of the source, suggesting
that there is no direct link between the macroscopic characteristics of the
coronal plasma and the the variability of the accretion flow. All spectra are
well fitted by a thermal comptonization component plus an extra high-energy
powerlaw. This confirms the presence of thermal and non-thermal electrons
around the black hole.Comment: 7 pages, 8 figures, 2 tables; accepted (09/11/2008) for publication
in A&
Ferromagnetism and conductivity in atomically thin SrRuO3
Atomically thin ferromagnetic and conducting electron systems are highly
desired for spintronics, because they can be controlled with both magnetic and
electric fields. We present (SrRuO3)1-(SrTiO3)5 superlattices and
single-unit-cell-thick SrRuO3 samples that are capped with SrTiO3. We achieve
samples of exceptional quality. In these samples, the electron systems comprise
only a single RuO2 plane. We observe conductivity down to 50 mK, a
ferromagnetic state with a Curie temperature of 25 K, and signals of magnetism
persisting up to approximately 100 K.Comment: The version published at Phys. Rev. X (open access) contains a large
amount of additional material compared to the version published her
Potentials of Mean Force for Protein Structure Prediction Vindicated, Formalized and Generalized
Understanding protein structure is of crucial importance in science, medicine
and biotechnology. For about two decades, knowledge based potentials based on
pairwise distances -- so-called "potentials of mean force" (PMFs) -- have been
center stage in the prediction and design of protein structure and the
simulation of protein folding. However, the validity, scope and limitations of
these potentials are still vigorously debated and disputed, and the optimal
choice of the reference state -- a necessary component of these potentials --
is an unsolved problem. PMFs are loosely justified by analogy to the reversible
work theorem in statistical physics, or by a statistical argument based on a
likelihood function. Both justifications are insightful but leave many
questions unanswered. Here, we show for the first time that PMFs can be seen as
approximations to quantities that do have a rigorous probabilistic
justification: they naturally arise when probability distributions over
different features of proteins need to be combined. We call these quantities
reference ratio distributions deriving from the application of the reference
ratio method. This new view is not only of theoretical relevance, but leads to
many insights that are of direct practical use: the reference state is uniquely
defined and does not require external physical insights; the approach can be
generalized beyond pairwise distances to arbitrary features of protein
structure; and it becomes clear for which purposes the use of these quantities
is justified. We illustrate these insights with two applications, involving the
radius of gyration and hydrogen bonding. In the latter case, we also show how
the reference ratio method can be iteratively applied to sculpt an energy
funnel. Our results considerably increase the understanding and scope of energy
functions derived from known biomolecular structures
Structure and dynamics of ring polymers: entanglement effects because of solution density and ring topology
The effects of entanglement in solutions and melts of unknotted ring polymers
have been addressed by several theoretical and numerical studies. The system
properties have been typically profiled as a function of ring contour length at
fixed solution density. Here, we use a different approach to investigate
numerically the equilibrium and kinetic properties of solutions of model ring
polymers. Specifically, the ring contour length is maintained fixed, while the
interplay of inter- and intra-chain entanglement is modulated by varying both
solution density (from infinite dilution up to \approx 40 % volume occupancy)
and ring topology (by considering unknotted and trefoil-knotted chains). The
equilibrium metric properties of rings with either topology are found to be
only weakly affected by the increase of solution density. Even at the highest
density, the average ring size, shape anisotropy and length of the knotted
region differ at most by 40% from those of isolated rings. Conversely, kinetics
are strongly affected by the degree of inter-chain entanglement: for both
unknots and trefoils the characteristic times of ring size relaxation,
reorientation and diffusion change by one order of magnitude across the
considered range of concentrations. Yet, significant topology-dependent
differences in kinetics are observed only for very dilute solutions (much below
the ring overlap threshold). For knotted rings, the slowest kinetic process is
found to correspond to the diffusion of the knotted region along the ring
backbone.Comment: 17 pages, 11 figure
Forward to the past: reinventing intelligence-led policing in Britain
Drawing on archival, secondary material and primary research, this paper examines 'Total Policing', the strategy recently adopted by London's Metropolitan Police. It situates that analysis within a critical examination of other innovative policing strategies previously employed in Britain. It argues that the prospects for Total Policing depend upon the resolution of long-standing problems such as: the inadequacy and inefficiency of local intelligence work; the paucity of evidence for the success of commanders' previous efforts to harness together the component parts of their forces in pursuit of a single mission; and, above all, a seeming inability to learn the lessons of the past. © 2013 © 2013 Taylor & Francis
Testing the theory of immune selection in cancers that break the rules of transplantation
Modification of cancer cells likely to reduce their immunogenicity, including loss or down-regulation of MHC molecules, is now well documented and has become the main support for the concept of immune surveillance. The evidence that these modifications, in fact, result from selection by the immune system is less clear, since the possibility that they may result from reorganized metabolism associated with proliferation or from cell de-differentiation remains. Here, we (a) survey old and new transplantation experiments that test the possibility of selection and (b) survey how transmissible tumours of dogs and Tasmanian devils provide naturally evolved tests of immune surveillance
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