2 research outputs found
Rapid and Self-Administrable Capillary Blood Microsampling Demonstrates Statistical Equivalence with Standard Venous Collections in NMR-Based Lipoprotein Analysis
We investigated plasma and serum blood derivatives from
capillary
blood microsamples (500 μL, MiniCollect tubes) and corresponding
venous blood (10 mL vacutainers). Samples from 20 healthy participants
were analyzed by 1H NMR, and 112 lipoprotein subfraction
parameters; 3 supramolecular phospholipid composite (SPC) parameters
from SPC1, SPC2, and SPC3 subfractions;
2 N-acetyl signals from α-1-acid glycoprotein
(Glyc), GlycA, and GlycB; and 3 calculated parameters, SPC (total),
SPC3/SPC2, and Glyc (total) were assessed. Using
linear regression between capillary and venous collection sites, we
explained that agreement (Adj. R2 ≥
0.8, p < 0.001) was witnessed for 86% of plasma
parameters (103/120) and 88% of serum parameters (106/120), indicating
that capillary lipoprotein, SPC, and Glyc concentrations follow changes
in venous concentrations. These results indicate that capillary blood
microsamples are suitable for sampling in remote areas and for high-frequency
longitudinal sampling of the majority of lipoproteins, SPCs, and Glycs
Integrative Molecular Structure Elucidation and Construction of an Extended Metabolic Pathway Associated with an Ancient Innate Immune Response in COVID-19 Patients
We present compelling
evidence for the existence of an extended
innate viperin-dependent pathway, which provides crucial evidence
for an adaptive response to viral agents, such as SARS-CoV-2. We show
the in vivo biosynthesis of a family of novel endogenous cytosine
metabolites with potential antiviral activities. Two-dimensional nuclear
magnetic resonance (NMR) spectroscopy revealed a characteristic spin-system
motif, indicating the presence of an extended panel of urinary metabolites
during the acute viral replication phase. Mass spectrometry additionally
enabled the characterization and quantification of the most abundant
serum metabolites, showing the potential diagnostic value of the compounds
for viral infections. In total, we unveiled ten nucleoside (cytosine-
and uracil-based) analogue structures, eight of which were previously
unknown in humans allowing us to propose a new extended viperin
pathway for the innate production of antiviral compounds.
The molecular structures of the nucleoside analogues and their correlation
with an array of serum cytokines, including IFN-α2, IFN-γ,
and IL-10, suggest an association with the viperin enzyme contributing
to an ancient endogenous innate immune defense mechanism against viral
infection