X-Ray Scattering at FeCo(001) Surfaces and the Crossover between Ordinary and Normal Transitions

In a recent experiment by Krimmel et al. [PRL 78, 3880 (1997)], the critical behavior of FeCo near a (001) surface was studied by x-ray scattering. Here the experimental data are reanalyzed, taking into account recent theoretical results on order-parameter profiles in the crossover regime between ordinary and normal transitions. Excellent agreement between theoretical expectations and the experimental results is found.Comment: 9 pages, Latex, 1 PostScript figure, to be published in Phys.Rev.

Does Glycosylation as a modifier of Original Antigenic Sin explain the case age distribution and unusual toxicity in pandemic novel H1N1 influenza?

Background: A pandemic novel H1N1 swine-origin influenza virus has emerged. Most recently the World Health Organization has announced that in a country-dependent fashion, up to 15% of cases may require hospitalization, often including respiratory support. It is now clear that healthy children and young adults are disproportionately affected, most unusually among those with severe respiratory disease without underlying conditions. One possible explanation for this case age distribution is the doctrine of Original Antigenic Sin, i.e., novel H1N1 may be antigenically similar to H1N1 viruses that circulated at an earlier time. Persons whose first exposure to influenza viruses was to such similar viruses would be relatively immune. However, this principle is not sufficient to explain the graded susceptibility between ages 20 and 60, the reduced susceptibility in children below age 10, and the unusual toxicity observed.Methods: We collected case data from 11 countries, about 60% of all cases reported through mid-July 2009. We compared sequence data for the hemagglutinin of novel H1N1 with sequences of H1N1 viruses from 1918 to the present. We searched for sequence differences that imply loss of antigenicity either directly through amino acid substitution or by the appearance of sites for potential glycosylation proximal to sites known to be antigenic in humans. We also considered T-cell epitopes.Results: In our composite, over 75% of confirmed cases of novel H1N1 occurred in persons ≤ 30 years old, with peak incidence in the age range 10-19 years. Less than 3% of cases occurred in persons over 65, with a gradation in incidence between ages 20 and 60 years.The sequence data indicates that novel H1N1 is most similar to H1N1 viruses that circulated before 1943. Novel H1N1 lacks glycosylation sites on the globular head of hemagglutinin (HA1) near antigenic regions, a pattern shared with the 1918 pandemic strain and H1N1 viruses that circulated until the early 1940s. Later H1N1 viruses progressively added new glycosylation sites likely to shield antigenic epitopes, while T-cell epitopes were relatively unchanged.Conclusions: In this evolutionary context, Original Antigenic Sin exposure should produce an immune response increasingly mismatched to novel H1N1 in progressively younger persons. We suggest that it is this mismatch that produces both the gradation in susceptibility and the unusual toxicity. Several murine studies suggest specific cell types as a likely basis of the unusual toxicity. These studies also point to widely available pharmaceutical agents as plausible candidates for mitigating the toxic effects. The principle of Original Antigenic Sin modified by glycosylation appears to explain both the case age distribution and the unusual toxicity pattern of the novel H1N1 pandemic. In addition, it suggests pharmaceutical agents for immediate investigation for mitigation potential, and provides strategic guidance for the distribution of pandemic mitigation resources of all types. © 2010 Reichert et al; licensee BioMed Central Ltd.published_or_final_versio

A search for X-ray emission from rich clusters, extended halos around clusters, and superclusters

The all-sky data base acquired with the HEAO A-2 experiment was searched for X-ray emission on a variety of metagalactic size scales which were either predicted or previously detected. Results in the 0.2-60 keV energy range are presented. The optically richest clusters, including those from which a microwave decrement were observed, appear to be relatively underluminous in X-rays. Observations of Abell 576 show its luminosity to be less than earlier estimates, and moreover less than the luminosity predicted from its microwave decrement, unless the intracluster gas is a factor of approximately 10 hotter than in typical clusters. Near SC0627 there are two X-ray sources, and the identification of the dominant source with SCO627 is probably incorrect. New spectral observations of Abell 401 and 2147, possible superclusters, reveal that they have typical cluster spectra with iron line emission

Colloidal hydrodynamic coupling in concentric optical vortices

Optical vortex traps created from helical modes of light can drive fluid-borne colloidal particles in circular trajectories. Concentric circulating rings of particles formed by coaxial optical vortices form a microscopic Couette cell, in which the amount of hydrodynamic drag experienced by the spheres depends on the relative sense of the rings' circulation. Tracking the particles' motions makes possible measurements of the hydrodynamic coupling between the circular particle trains and addresses recently proposed hydrodynamic instabilities for collective colloidal motions on optical vortices.Comment: 7 pages, 2 figures, submitted to Europhysics Letter

Pan-European grading scales: lessons from national systems and the ECTS

This article assesses the impact of the Bologna Process on the grading schemes of EU member countries. In light of some problems regarding the implementation of the European Credit Transfer system (ECTS), the author proposes further reforms and offers some elements of a unified grading system for European higher education. The author explores the variation among Europe’s grading systems and the resulting lessons learned are shared here. Lastly, this article also argues that principles of justice and fairness, deemed central to academic freedom, are best upheld by the use of a unified grading system at national and European levels

SNP genotyping using alkali cleavage of RNA/DNA chimeras and MALDI time-of-flight mass spectrometry

Single nucleotide polymorphisms (SNPs) are now widely used for many DNA analysis applications such as linkage disequilibrium mapping, pharmacogenomics and traceability. Many methods for SNP genotyping exist with diverse strategies for allele-distinction. Mass spectrometers are used most commonly in conjunction with primer extension procedures with allele-specific termination. Here we present a novel concept for allele-preparation for SNP genotyping. Primer extension is carried out with an extension primer positioned immediately upstream of the SNP that is to be genotyped, a complete set of four ribonucleotides and a ribonucleotide incorporating DNA polymerase. The allele-extension products are then treated with alkali, which results in the cleavage immediately after the first added ribonucleotide. In addition, to obtain fragments easily detectable by mass spectrometry, we have included a ribonucleotide in the primer usually at the fourth nucleotide from the 3′ terminus. The method was tested on four SNPs each with a different combination of nucleotides. The advantage over other mass spectrometry-based SNP genotyping assays is that this one only requires a PCR, a primer extension reaction with a universal extension mix and an inexpensive facile cleavage reaction, which makes it overall very cost effective and easy in handling

Refactoring Process Models in Large Process Repositories.

With the increasing adoption of process-aware information systems (PAIS), large process model repositories have emerged. Over time respective models have to be re-aligned to the real-world business processes through customization or adaptation. This bears the risk that model redundancies are introduced and complexity is increased. If no continuous investment is made in keeping models simple, changes are becoming increasingly costly and error-prone. Though refactoring techniques are widely used in software engineering to address related problems, this does not yet constitute state-of-the art in business process management. Process designers either have to refactor process models by hand or cannot apply respective techniques at all. This paper proposes a set of behaviour-preserving techniques for refactoring large process repositories. This enables process designers to eectively deal with model complexity by making process models better understandable and easier to maintain

Towards unified understanding of conductance of stretched monatomic contacts

When monatomic contacts are stretched, their conductance behaves in qualitatively different ways depending on their constituent atomic elements. Under a single assumption of resonance formation, we show that various conductance behavior can be understood in a unified way in terms of the response of the resonance to stretching. This analysis clarifies the crucial roles played by the number of valence electrons, charge neutrality, and orbital shapes.Comment: 2 figure