17 research outputs found

    Lineage Switch Macrophages Can Present Antigen

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    Recent reports of “lineage switching” from a lymphoid to macrophage phenotype have left unresolved the question of whether such cells are functional macrophages or nonfunctional products of differentiation gone awry. This study demonstrates that several “macrophage-like” cell lines derived from v-Ha-ras-transformed pre-B cells have gained the capacity to effectively present antigen in MHC-restricted fashion. Using an assay involving the cocultivation of putative antigen-presenting cells with chicken ovalbumin (cOVA) and a cOVA-specific T-cell hybridoma, “lineage switch” cell lines were found to present antigen as effectively as macrophage-containing peritoneal exudates. Neither the original pre-B-cell precursors nor B-cell lymphomas derived from them present antigen. Thus, we have demonstrated that these “lineage switch” macrophages are capable of antigen presentation, a mature differentiated function. While gaining macrophage characteristics, these cells have also rearranged their kappa light-chain immunoglobulin locus, suggesting that macrophage differentiation and immunoglobulin rearrangement are not mutually exclusive processes. The existence of both lymphoid and myeloid characteristics in a cell fully capable of antigen presentation suggests greater plasticity in hematopoietic lineage commitment than conventionally thought to be the case

    Reengineering the Marine Corps rifle range

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    With no significant changes in the design of rifle ranges in more than 100 years, the current range systems are not keeping pace with technological advancements. The Marine Corps rifle ranges are manpower and material intensive, requiring unit commanders to lose personnel to the training evolution for extended periods of non-productive time. Manual target operation, excessive transition time, and extra duties all contribute to eight to ten hours per day to accomplish one hour of live-fire training per individual Marine. Marines must remain at the range to act as scorekeepers, target makers, and/or target operators when not assigned to shoot. The design and implementation of an automated range system with capabilities specifically designed to operate, score, mark, and maintain targets would reduce the non-productive time a Marine spends on the rifle range. Results from this comparative analysis indicate that the automated range would reduce man-hours by seventy-five percent. Furthermore, the implementation of computer technology will enable instructors and shooters to better analyze each training evolution.http://archive.org/details/reengineeringmar109459310U.S. Marine Corps (U.S.M.C.) author.Approved for public release; distribution is unlimited
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