Genetic studies of the marine penaeid prawn Penaeus monodon Fabricius, 1798 (TH 119)

The thesis contains the detailed aspects of an investigation entitled "Genetic Studies of the Marine Penaeid prawn Penaeus monodon Fabricius. 1798". The reasons for selecting this particular research problem. the three research methods for investigating the problem and the reasons for selecting Penaeus monodon as the candidate species are highlighted in the introductory chapter. The published informations relevant to the topic of the present investigation and the methods of investigation were reviewed separately under the sub headings: Morphometrics, Biochemical genetics and Molecular genetics. The standardized procedures followed for collection of morphometric. biochemical genetic and molecular genetic data and the statistical procedures for interpretation of these separate data were given under the chapter Materials and Methods. The data obtained under each method were objectively analyzed using fIgures and tables prepared for the purpose. The detailed aspects of the results were presented under the chapter Results. The highlights of the results are given below

Morphometric structure of the jumbo tiger prawn, Penaeus monodon Fabricius, 1798 from southeast and southwest coasts of India

Morphometric variables were measured to detect variation among the random population samples of Penaeus monodon from east and west coast - Kochi, Calicut, Mangalore, Karwar, Kakinada and Chennai of India. Among these variables, PCL showed the highest correlation with the tail weight (TLW) in both males (0.9605) and females (0.9639). Truss network analysis of 26 measurements from the six centres were log transformed and were subjected to Principal Component Analysis and accounted a total of 89.15% variations in truss measurements data and showed no separate cluster formation in the plot of sheared PC scores and hence confirm homogeneous stock structure

Genetic Studies of the Marine Penaeid Prawn, Penaeus Monodon Fabricius, 1798

The thesis deals with the results of the study of the population characteristics of the marine penaeid prawn, Penaeus monodon from South India. The present findings on the morphometric and biochemical genetic structure support the hypothesis that the populations of P.monodon of South India have homogeneous stock structure. To the contrary, the significantly different random amplified polymorphic DNA (RAPD) profiles in samples of Kochi and Chennai support the hypothesis that east and west cost populations of P.monodon are separate stocks

Investigation on the molecular genetic profile of Indian marine penaeid prawn, Penaeus monodon Fabricius, 1798

The random amplified polymorphic DNA (RAPD) technique was used to examine the genetic variability in east and west coast penaeid prawn, P. monodon from two wild natural stocks (Chennai and Kochi). Initially, 20 oligonucleotide primers (OPA 1- 20, Bangalore Genei) were screened and only OPA-2 & 4 were selected to amplify DNA samples from these two sites. A total of 16 fractions for OPA-2 and 15 fractions for OPA-4, ranging in size from 0.0025 to 1.9 kb were scored. The unique RAPD fragments (markers) were detected by primer OPA-2 in Kochi population (0.1, 0.2, 0.8, 0.9, 0.95, 1.3, 1.9 kb) and OPA-4 from Chennai were revealed two unique fragments each in Kochi (0.80, 1.6 kb) and Chennai (0.0025, 0.95 kb) indicates that these two populations may be distinct genetic stocks. This data may provide baseline information on the genetic variation from one site each of east and west coast of Indian penaeid prawn, P. monodon sample populations for future management of the resourc

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Not AvailableMorphometric variables were measured to detect variation among the random population samples of Penaeus monodon from east and west coast - Kochi, Calicut, Mangalore, Karwar, Kakinada and Chennai of India. Among these variables, PCL showed the highest correlation with the tail weight (TLW) in both males (0.9605) and females (0.9639). Truss network analysis of 26 measurements from the six centres were log transformed and were subjected to Principal Component Analysis and accounted a total of 89.15% variations in truss measurements data and showed no separate cluster formation in the plot of sheared PC scores and hence confirm homogeneous stock structure.Not Availabl