Engineering of zinc finger and MHC motifs to locked-in tertiary folds

The assembly of helical and β-sheet peptide blocks containing reactive chain ends results inhighly branched chain architectures (‘locked-in folds') mimicking native tertiary structures.This molecular kit strategy allows to bypass the protein folding problem in protein de novodesign and gives access to protein mimetics of high thermodynamic stability. The validity ofthis concept is exemplified for the design and synthesis of locked-in folds mimicking the zincfinger and MHC folding motif

Engineering of zinc finger and MHC motifs to locked-in tertiary folds

The assembly of helical and b-sheet peptide blocks contg. reactive chain ends results in highly branched chain architectures (\"locked-in folds\") mimicking native tertiary structures. This mol. kit strategy allows to bypass the protein folding problem in protein de novo design and gives access to protein mimetics of high thermo-dynamic stability. The validity of this concept is exemplified for the design and synthesis of locked-in folds mimicking the zinc finger and MHC folding motifs. [on SciFinder (R)

Engineering of zinc finger motifs to \"locked-in tertiary folds\"

A symposium report on the solid-phase prepn. of template cyclopeptide cyclo[Lys(R)-Pro-Gly-Cys-Asp-Arg-Lys-Lys(R)-Pro-Gly-Phe-Ala-Cys-Ala] (R = COCH2ONH2) and helical 18-mer peptide aldehyde R1-Phe-Ser-Arg-Ser-Asp-Glu-Leu-Thr-Arg-His-Ile-Arg-Ile-His-Thr-Gly-Lys(R1)Gly-OH (R1 = COCHO) and double oxime formation of the template with the peptide aldehyde as a zinc finger DNA binding protein mimic. The synthetic peptide construct retains some helical structure even in the absence of zinc, as shown by CD. [on SciFinder (R)

Template assembled synthetic proteins (TASP) as functional mimetics of proteins

A review with 26 refs. Methodologies for the synthesis of functional TASP compds. are given. [on SciFinder (R)

Template assembled synthetic peptides (TASP) as receptor mimetics and \"locked-in\" tertiary folds

A symposium report. A new concept for the construction of protein mimetics based on the sepn. of functional and structural domains in proteins has been evaluated by the successful design and total synthesis of two prototype TASP mols. [on SciFinder (R)