Detection of Antibodies Against Trypanosoma evansi in Sheep by Indirect ELISA in Rayalaseema Region of Andhra Pradesh

The present research was carried out with an objective to improve the diagnostic tools for detection of antibodies against Trypanosoma evansi infection using indirect enzyme-linked immunosorbent assay (ELISA) in sheep. In this study standardized the Indirect ELISA for detection of T. evansi in sheep. The optimum concentration of antigen, test sera and conjugate were determined as 5µg per well, 1:10 and 1: 4000 dilutions, respectively. 464 serum samples were collected from sheep in different parts of the Rayalaseema region of Andhra Pradesh for screening of T. evansi infection. Out of 464 serum samples 46 (9.91%) were found positive by indirect ELISA

Histopathological Observations in Rabbits Experimentally Infected with Trypanosoma evansi

New Zealand white rabbits (n=6) were challenged with the South Indian local strain of Trypanosoma evansi. Each animal was infected with 5x105 trypanosomes subcutaneously. Animals were daily examined for the development of clinical signs using wet blood-films sampled from the ear veins. Clinically, intermittent pyrexia, undulating parasitaemia, anorexia and emaciation were predominant. Three months post infection, rabbits were sacrificed, detailed postmortem examination was carried out and representative tissue samples were fixed. Gross pathological changes including paleness of visceral organs, gelatinization of fat, congested and oedematous lungs,mucoid enteritis, hepatomegaly and splenomegaly were noticed. Histopathologically, internal organs elucidated clear changes consisted of severe hepatic fatty change, pulmonary congestion with thickened interstitial spaces and emphysema, degeneration of the renal epithelium associated with cystic tubular formation and congested red pulp

Efficacy of Enrofloxacin in the Treatment of Recurrent Pyoderma in Dogs

Dogs with a history of more than three episodes of skin infections in a period of one year were selected for a study on recurrent pyoderma. Oral enrofloxacin along with appropriate simultaneous medication for the underlying associated conditions were chosen as therapy for recurrent pyoderma in dogs. Response to therapy was excellent in all the cases. Improvement was noticed by 12 to 20 days and 20 to 26 days in recurrent superficial and deep pyoderma respectively. Relapse occurred in one dog by 45 days due to re-introduction of allergic food. Enrofloxacin proved to be an effective, safe and convenient antibiotic for the treatment of recurrent pyoderma in dogs

Detection of Antibodies Against Trypanosoma evansi in Sheep by Indirect ELISA in Rayalaseema Region of Andhra Pradesh

The present research was carried out with an objective to improve the diagnostic tools for detection of antibodies against Trypanosoma evansi infection using indirect enzyme-linked immunosorbent assay (ELISA) in sheep. In this study standardized the Indirect ELISA for detection of T. evansi in sheep. The optimum concentration of antigen, test sera and conjugate were determined as 5µg per well, 1:10 and 1: 4000 dilutions, respectively. 464 serum samples were collected from sheep in different parts of the Rayalaseema region of Andhra Pradesh for screening of T. evansi infection. Out of 464 serum samples 46 (9.91%) were found positive by indirect ELISA

Histopathological Observations in Rabbits Experimentally Infected with Trypanosoma evansi

New Zealand white rabbits (n=6) were challenged with the South Indian local strain of Trypanosoma evansi. Each animal was infected with 5x105 trypanosomes subcutaneously. Animals were daily examined for the development of clinical signs using wet blood-films sampled from the ear veins. Clinically, intermittent pyrexia, undulating parasitaemia, anorexia and emaciation were predominant. Three months post infection, rabbits were sacrificed, detailed postmortem examination was carried out and representative tissue samples were fixed. Gross pathological changes including paleness of visceral organs, gelatinization of fat, congested and oedematous lungs,mucoid enteritis, hepatomegaly and splenomegaly were noticed. Histopathologically, internal organs elucidated clear changes consisted of severe hepatic fatty change, pulmonary congestion with thickened interstitial spaces and emphysema, degeneration of the renal epithelium associated with cystic tubular formation and congested red pulp

Efficacy of Enrofloxacin in the Treatment of Recurrent Pyoderma in Dogs

Dogs with a history of more than three episodes of skin infections in a period of one year were selected for a study on recurrent pyoderma. Oral enrofloxacin along with appropriate simultaneous medication for the underlying associated conditions were chosen as therapy for recurrent pyoderma in dogs. Response to therapy was excellent in all the cases. Improvement was noticed by 12 to 20 days and 20 to 26 days in recurrent superficial and deep pyoderma respectively. Relapse occurred in one dog by 45 days due to re-introduction of allergic food. Enrofloxacin proved to be an effective, safe and convenient antibiotic for the treatment of recurrent pyoderma in dogs