2 research outputs found
Sistema de administraci贸n oral del toxoide tet谩nico
Systemic and local immune response against tetanus toxoid (TT) encapsulated in chitosan (CS) microparticles (CSTT)prepared by ionic cross-linking using Sodium Tripolyphosphate (STPP) was studied. CS-TT microparticles, TTin PBS and plain CS microparticles were orally administered to mice and TT (adsorbed) was administered throughintramuscular route. Sera were analyzed for anti-TT IgG and intestinal lavage for anti-TT IgA levels using an ELISA.The study shows the effi cacy of chitosan microparticle suspension system, containing a TT, in inducing the IgA inintestine and IgG in systemic circulation.Se ha estudiado la respuesta sist茅mica e inmune local frente al toxoide tet谩nico (TT) encapsulado en micropart铆culasde quitosano (CS), (CS-TT) preparado mediante reticulaci贸n i贸nica con tripolifosfato de sodio (STPP). Se administrarona ratones micropart铆culas CS-TT, TT en tamp贸n de fosfato salino (PBS) y micropart铆culas CS simples, y seadministr贸 TT (adsorbido) por v铆a intramuscular. Se analiz贸 el suero para determinar los niveles de IgG anti-TTy se realiz贸 un lavado intestinal para determinar los niveles de IgA anti-TT mediante la t茅cnica ELISA. El estudiodemuestra la efi cacia del sistema de suspensi贸n de micropart铆culas de quitosano, con TT, para inducir IgA en elintestino e IgC en la circulaci贸n sist茅mica