Ameliorative effect of sixteen weeks endurance training on biochemical and oxidative damage in high fat diet induced obese rats

Obesity is mainly caused by consumption of high fat diet (HFD) and a lack of physical activity. Physical activity is an efficient strategy to delay development of obesity. In this studyv we tried to evaluate attenuating properties of 16 weeks endurance training on plasma oxidative stress and some biochemical parameters in HFD induced obese rats. Twenty-four male Wistar rats were randomly divided into four groups: standard diet group (SD), standard diet with endurance training group (ESD), HFD group and HFD with endurance training group (EHFD). After sixteen weeks, plasma was prepared and evaluated for measurement of different parameters. The results showed that HFD significantly decreased the activities of superoxide dismutase (33.58%), catalase (26.51%) and glutathione S-transferase (22.77%), while endurance training increased these enzymes activities. However, exercise ameliorated the increased malondialdehyde level and depletion of glutathione. In addition, it significantly reduced the increased levels of liver enzymes activities and lipid profiles. These findings suggest that endurance training has found to have beneficial effects against HFD-induced oxidative damage through increasing reduced antioxidants levels and inhibition of lipid peroxidation due to its antioxidant property. Thus, it can be considered an interesting strategy for the management of obesity related diseases

Effect of eight weeks high-fat diet with endurance training on plasma levels of Amylin in male Wistar rats

Background: Amylin is a polypeptide hormone prevents weight gain and obesity by loss of appetite. Therefore, the purpose of this study was the effect of eight weeks high-fat diet with endurance training on body weight and plasma levels of amylin in male wistar rats. Materials and Methods: In this experimental study, 24 young male Wistar rats were divided into three groups (n=8) includes control (C), high fat diet + training (ET+HFD) and high fat diet (HFD) groups in age range 50 to 60 days and average weight 160&plusmn;10 g. The (ET+HFD) group rats run on treadmill without slope by 5 day/week, per session 20-63 min, 15-30 m/min velocity for eight weeks and they used a high fat diet. The control group used for initial sampling and high fat diet (HFD) group no train. High fat diet was included 60% fat, 24% CHO and 16% protein available two groups; C and ET. Blood sampling was conducted after latest training session with 12h fasting. The amylin concentration was measured by Cusabio Biotech elisa kit. The collected data were analyzed using the ANOVA and Tukey Post-Hoc tests (P<0/05). Results: There were no significant difference between amylin levels in (ET+HFD) and (HFD) groups compared to control (C) group (p=0/25, p=0/867). The reduction of amylin concentration was no significant in (ET+HFD) group than HFD group (p=0/543). The rats' body weight were higher significantly in ET+HFD and HFD than control group (p<0/0001). No difference significant appears between body weight in ET+HFD and HFD group rats (p=0/795). Conclusion: It seems that high fat diet for eight weeks with and without endurance exercise training does not change plasma level of amylin concentration in rats. However, high fat diet increase rats body weight and eight weeks endurance training was not able to reduce body weight in high fat diet-fed rats

Ameliorative effect of sixteen weeks endurance training on biochemical and oxidative damage in high fat diet induced obese rats

107-115Obesity is mainly caused by consumption of high fat diet (HFD) and a lack of physical activity. Physical activity is an efficient strategy to delay development of obesity. In this studyv we tried to evaluate attenuating properties of 16 weeks endurance training on plasma oxidative stress and some biochemical parameters in HFD induced obese rats. Twenty-four male Wistar rats were randomly divided into four groups: standard diet group (SD), standard diet with endurance training group (ESD), HFD group and HFD with endurance training group (EHFD). After sixteen weeks, plasma was prepared and evaluated for measurement of different parameters. The results showed that HFD significantly decreased the activities of superoxide dismutase (33.58%), catalase (26.51%) and glutathione S-transferase (22.77%), while endurance training increased these enzymes activities. However, exercise ameliorated the increased malondialdehyde level and depletion of glutathione. In addition, it significantly reduced the increased levels of liver enzymes activities and lipid profiles. These findings suggest that endurance training has found to have beneficial effects against HFD-induced oxidative damage through increasing reduced antioxidants levels and inhibition of lipid peroxidation due to its antioxidant property. Thus, it can be considered an interesting strategy for the management of obesity related diseases

Relationship between TCF7L2 Relative Expression in Pancreas Tissue with Changes in Insulin by High Intensity Interval Training (HIIT) in Type 2 Diabetes Rats

Introduction: Both environmental and genetic factors have been implicated in the development of type 2 diabetes (T2D). The objectives of the present study were: 1) to investigate the effect of high intensity interval training (HIIT) on fasting glucose, insulin and TCF7L2 expression in pancreas tissue of T2D rats, 2) to determine the relation between TCF7L2 expression with insulin changes in the HIIT and control groups. Methods: In the present applied-experimental study, T2D male Wistar rats induced by intraperitoneal streptozotocin-nicotinamide were assigned to control (no-training) and HIIT (5&nbsp;times/week/12-week) groups. Fasting glucose, serum insulin and TCF7L2 expression in pancreas tissues of both groups were measured after lasted exercise and compared between 2 groups by independent T test. Also, the relation between TCF7L2 expression and insulin of HIIT to the control group was assessed by Pearson correlations. Results: The HIIT training in the training group was associated with improved fasting glucose compared with the control group (P<0.001). A significant increase was observed in serum insulin levels (P< 0.001). Also, there was seen a significant decrease in TCF7L2 expression in pancreas tissues in HIIT group compared with the control group (P= 0.038). Significant negative correlation was found between TCF7L2 expression and insulin changes of the HIIT to control groups (r=0.84, P=0.034). conclusion: HIIT training is associated with improvements in glycemic control and insulin secretion in T2D rats. Based on these data, this improvement can be attributed to decrease in TCF7L2 expression at pancreas tissues by HIIT training

Effect of eight weeks high-fat diet with endurance training on plasma levels of Amylin in male Wistar rats

Background: Amylin is a polypeptide hormone prevents weight gain and obesity by loss of appetite. Therefore, the purpose of this study was the effect of eight weeks high-fat diet with endurance training on body weight and plasma levels of amylin in male wistar rats. Materials and Methods: In this experimental study, 24 young male Wistar rats were divided into three groups (n=8) includes control (C), high fat diet + training (ET+HFD) and high fat diet (HFD) groups in age range 50 to 60 days and average weight 160&plusmn;10 g. The (ET+HFD) group rats run on treadmill without slope by 5 day/week, per session 20-63 min, 15-30 m/min velocity for eight weeks and they used a high fat diet. The control group used for initial sampling and high fat diet (HFD) group no train. High fat diet was included 60% fat, 24% CHO and 16% protein available two groups; C and ET. Blood sampling was conducted after latest training session with 12h fasting. The amylin concentration was measured by Cusabio Biotech elisa kit. The collected data were analyzed using the ANOVA and Tukey Post-Hoc tests (P<0/05). Results: There were no significant difference between amylin levels in (ET+HFD) and (HFD) groups compared to control (C) group (p=0/25, p=0/867). The reduction of amylin concentration was no significant in (ET+HFD) group than HFD group (p=0/543). The rats' body weight were higher significantly in ET+HFD and HFD than control group (p<0/0001). No difference significant appears between body weight in ET+HFD and HFD group rats (p=0/795). Conclusion: It seems that high fat diet for eight weeks with and without endurance exercise training does not change plasma level of amylin concentration in rats. However, high fat diet increase rats body weight and eight weeks endurance training was not able to reduce body weight in high fat diet-fed rats

The effect of eight weeks endurance training and high-fat diet on appetite-regulating hormones in rat plasma

Objective(s):Consumption of high-fat foods is one of the major causes of obesity. Physical exercise is a strategy used to counteract obesity. The aim of this study was to investigate the effect of eight weeks endurance training and high-fat diet (HFD) on appetite-regulating hormones in rat plasma. Materials and Methods:Twenty eight male Wistar rats were randomly divided into four groups: Control group with standard diet (CSD), endurance training with a standard diet (ESD), control group with high-fat diet (CHFD) and endurance training with high-fat diet (EHFD). Twenty-four hr after the last training session, the blood samples were obtained and analyzed for hormones levels. Results: The significant increased weight gain and food intake and decreased plasma nesfatin-1 and PYY3-36 levels were observed in CHFD group, while exercise under the HFD antagonized these effects. There were no significant changes in ghrelin, insulin and leptin levels in different groups. Conclusion: These results suggest that exercise can prevent fattening effect of HFD. Probably, performing exercise makes a reduction of food intake and weight gain in rat via the increase in nesfatin-1 and PYY levels. However, further studies are necessary to understand the exact mechanisms involved in this field