NeuroSAFE frozen section during robot-assisted radical prostatectomy (RARP): Peri-operative and Histopathological Outcomes from the NeuroSAFE PROOF Feasibility Randomised Controlled Trial

Objectives: To report on the methods, peri‐operative outcomes and histopathological concordance between frozen and final section from the NeuroSAFE PROOF Feasibility study (NCT03317990). Patients and Methods: Between May 2018 and March 2019 49 men at 2 UK centres underwent robot‐assisted robotic prostatectomy (RARP). 25 men were randomised to NeuroSAFE RARP (intervention arm) vs. 24 men to standard RARP (control arm). Frozen section was compared to final paraffin section margin assessment in the 25 men in the NeuroSAFE arm. Operation timings and complications were collected prospectively in both arms. Results: 50 NVB from 25 patients in the NeuroSAFE arm were analysed. When analysed by each pathological section (n=250, average 5 per side) we note sensitivity 100%, specificity 99.2%, AUC was 0.994 (95% CI 0.985 to 1, P= <.001). On an NVB basis (n=50) we note sensitivity of 100%, specificity 92.7%, and AUC of 0.963 (95% CI 0.914 to 1, p = <0.001. NeuroSAFE RARP lasted a mean 3 hours 16 minutes (knife to skin to off table, 95% CI 3 hrs 2 mins ‐ 3 hrs 30 mins) compared to 2 hours 14 minutes (2 hrs 2 mins ‐ 2 hours 25 mins, P=<0.001) for standard RARP. There was no morbidity associated with the additional length of operation in the NeuroSAFE arm. Conclusion: This feasibility study demonstrates the safety, the reproducibility and the excellent histopathological concordance of the NeuroSAFE technique in the NeuroSAFE PROOF trial. Though the technique increases the duration of RARP, this does not cause short‐term harm. Confirmation of feasibility has led to the opening of the fully powered NeuroSAFE PROOF RCT, which is currently underway at 4 sites in the UK

ATTENTION STATE OF CHILDREN OF HIGH SCHOOL AGE FOR DIFFERENT TYPES OF WEATHER

Attention provides cognitive processes and is a necessary moment of knowledge acquisition, high quality and productivity of mental work. The aim of the study – to learn the state of attention among schoolchildren of 15–17 years, depending on sex, level of neurotism and extra-introverts under different types of weather. Materials and Methods. 90 practically healthy students were examined. The proof-reading test that was used in our research is a common method of studying attention. The surveys were conducted on days with I, II and III types of weather. This article analyzes the indicators of attention such as the number of correctly drawn letters (m), the productivity of work (B), and the accuracy of attention (K). The integral index of attention stability (A) among the students of the senior school age for different types of weather was established to determine the level of neuroticism and extra-introversion. We used the teen version of the questionnaire Eisenck. The calculations of the results were carried out according to Student's t-criterion and methods of non-parametric statistics. Results and Discussion. The number of highlighted letters in senior pupils decreases with deteriorating of weather conditions. The obtained data indicate a significant decline in the productivity of attention in students of 9–11 classes with deterioration of weather conditions. Accuracy and stability of attention were the best under the favorable weather, decreased under conditionally unfavorable and were the minimum value in unfavorable weather. Analyzing the results, it was found out that the group of people with high neuroticism is the most sensitive to weather conditions, and students with low neuroticism are more stable. According to the conducted research, introverts-senior pupils tended to increase the state of attention in comparison with extroverts. Conclusions. It is established that the state of attention is the best with the type of weather for all children of the senior school age. Unfavorable weather had a negative effect on the state of attention of the pupils of the surveyed contingent. The indicators of attention decreased with deterioration of weather conditions compared with students with low neurotism in people with a high level of neurotism. We have found out that the indicators of attention decreased, in the pupils of the senior school age, regardless of extra-introversion

Species share of Molinio-Arrhenatheretea class in vegetation of the Forest Arboretum in Zielonka near Poznań and in adjacent treestands

Experimental-Research Unit – Forest Arboretum in Zielonka is located in the Puszcza Zielonka Landscape Park near Poznań and is bordering experimental forests of the Poznań University of Life Sciences. The aim of the studies was to identify the diversity of flora and vegetation of green areas in 13 landscape units, distinguished on the basis of the natural lie of the land, as well as forms and intensity of human impacts. 536 vascular plants species, including 82 of Molinio-Arrhenatheretea class and 102 plant communities with 13 of green areas therein were identified. Taxa and syntaxa of the class mentioned above are present in nearly all complexes of plant communities. The largest acreage of meadows and pastures belongs to the complex of green areas, where the presence of 57 species of Molinio-Arrhenatheretea class and seven associations were revealed. The lowest number of such indicators was found in aquatic and rush vegetation complex of water basins and in ruderal vegetation of build-up areas and roads, whereas their absence in watercourses vegetation complex. Characteristic species share of Molinia and fresh green areas is highly variable in analysed delimitations

Comparison of neonatal transient evoked otoacoustic emission responses recorded with linear and QuickScreen protocols

Linear and QuickScreen (non-linear) transient evoked otoacoustic emission (TEOAE) protocols were compared in terms of standardized clinical parameters in order to define the protocol producing recordings with the highest signal quality. Neonatal responses (520) were obtained from three different screening sites. The linear recordings were evoked by 69 and 75-dB p.e. SPL clicks. All responses were post-windowed by a 3.5- to 12.5-ms window, chosen by time-frequency analysis as the segment representing 97.35 per cent (linear) and 95.6 per cent (quick) of the total cumulative spectral energy. Evidence from hearing loss cases and the high similarity between the profile contours of the QuickScreen and the linear normal recordings have strongly suggested that a linear response evoked by a 75-dB p.e. SPL stimulus and post-processed by a 3.5 to 12.5 window is free of stimulus artefacts. The data indicate that the 75-dB linear protocol produces higher signal to noise ratios at 2.0, 3.0, and 4.0 kHz, higher wave reproducibility, and lower TEOAE noise values than the QuickScreen protocol

Assessment of auditory processing in children with dyslalia

The objective of the work was to assess occurrence of central auditory processing disorders in children with dyslalia. Material and method. The material included 30 children at the age 798 years old being under long-term speech therapy care due to articulation disorders. All the children were subjected to the phoniatric and speech examination, including tonal and impedance audiometry, speech therapist&#039;s consultation and psychologist&#039;s consultation. Electrophysi-ological (N2, P2, N2, P2, P300 record) and following psychoacoustic test of central auditory functions were performed (Frequency Pattern Test). Results. Analysis of the results revealed disorders in the process of sound analysis within frequency and P300 wave latency prolongation in children with dyslalia. Conclusions. Auditory processing disorders may be significant in development of correct articulation in children, they also may explain unsatisfactory results of long-term speech therap

PEOPLE: PatiEnt prOstate samPLes for rEsearch, a tissue collection pathway utilizing magnetic resonance imaging data to target tumor and benign tissue in fresh radical prostatectomy specimens.

BACKGROUND: Over 1 million men are diagnosed with prostate cancer each year worldwide, with a wide range of research programs requiring access to patient tissue samples for development of improved diagnoses and treatments. A random sampling of prostate tissue is sufficient for certain research studies; however, there is growing research need to target areas of the aggressive tumor as fresh tissue. Here we set out to develop a new pathway "PEOPLE: PatiEnt prOstate samPLes for rEsearch" to collect high-quality fresh tissue for research use, using magnetic resonance imaging (MRI) to target areas of tumor and benign tissue. METHODS: Prostate tissue was sampled following robotic radical prostatectomy, using MRI data to target areas of benign and tumor tissue. Initially, 25 cases were sampled using MRI information from clinical notes. A further 59 cases were sampled using an optimized method that included specific MRI measurements of tumor location along with additional exclusion criteria. All cases were reviewed in batches with detailed clinical and histopathological data recorded. For one subset of samples, DNA was extracted and underwent quality control. Ex vivo culture was carried out using the gelatin sponge method for an additional subset. RESULTS: Tumor was successfully fully or partially targeted in 64% of the initial cohort and 70% of the optimized cohort. DNA of high quality and concentration was isolated from 39 tumor samples, and ex vivo culture was successfully carried out in three cases with tissue morphology, proliferation, and apoptosis remaining comparable before and after 72 hours culture. CONCLUSION: Here we report initial data from the PEOPLE pathway; using a method for targeting areas of tumor within prostate samples using MRI. This method operates alongside the standard clinical pathway and minimizes additional input from surgical, radiological, and pathological teams, while preserving surgical margins and diagnostic tissue.Genomics England 100,000 genomes projectProstate Cancer U