Phylogenetic tree of 16S rRNA gene in cyanobacteria generated through BLASTN search in NCBI cyanobacterial database using the neighbor joining algorithm provided by MEGA 5.

<p>Phylogenetic tree of 16S rRNA gene in cyanobacteria generated through BLASTN search in NCBI cyanobacterial database using the neighbor joining algorithm provided by MEGA 5.</p

Effect of <i>Arthrospira</i> sp. CCC729 crude extract on selected cancer biomarkers.

<p>Multiplex assay showing levels of expression of cancer biomarkers in A498 cancer cells after treatment with crude extract at 100 μg·mL^-1 in the cells.</p

Effect of <i>Geitlerinema</i> sp. CCC728 crude extract on selected cancer biomarkers.

<p>Multiplex assay showing levels of expression of cancer biomarkers in A498 cancer cells after treatment with crude extract at 100 μg·mL^-1 in the cells showing impact on selected biomarkers.</p

Screening for anticancer activity on NRK52E cell line.

<p>Effects of crude extracts and TLC fractions on normal rat kidney epithelial (NRK52E) cells. <i>Geitlerinema</i> sp. CCC728 crude extract and TLC fractions C2, C3, C4, and C5 (a), <i>Arthrospira</i> sp. CCC729 crude extract and TLC fractions D1, D2, D3, and D4 (b). Significant difference (p<0.001) between pairs, control with crude, C2, C3, C4 and C5, crude with control, C2, C3 and C4. C2 with control, crude and C3 <b>(a),</b> Control with crude, D1, D2, D3 and D4 <b>(b)</b>.</p

Cell cycle analysis of A498 cell lines with PI staining using Flow cytometry.

<p>Interaction of cells with most potent fractions from the second TLC purification: control cells without treatment (a), cells <i>vs</i> D3 <b>(b)</b> and D4 <b>(c) from</b><i>Arthrospira</i>, C4 <b>(c)</b> and C5 <b>(d)</b> from <i>Geitlerinema</i>.</p

Screening for anticancer activity on MCF10A cell line.

<p>Effects of crude extracts and TLC fractions on normal human mammary epithelial (MCF10A) cells. Significant difference (p<0.001) between pairs, control with <i>Geitlerinema</i> CCC728 and <i>Arthrospira</i> CCC729 crude extracts <b>(a),</b> no significant difference (p>0.05) among pairs control and C2 and C3, C4 as well as C5 <b>(b)</b> and significant difference (p<0.05) among control with D2 with D3 and no significant difference (p>0.05) among pairs control with D1 and D4 <b>(c)</b>.</p

Apoptotic analysis by Flow cytometry using Annexin V-PI.

<p>A498 as well as MCF-10A cells were exposed to TLC fractions C4 and C5 (<i>Geitlerinema</i> sp. CCC728), D3 and D4 (<i>Arthrospira</i> sp. CCC729). Statistical analysis of A498 cell lines under treatment showed significant impact on cancer cells (F_4,10 = 106.582, p<0.001) <b>(a).</b> As far as MCF-10A cells were concerned they were not significantly affected by fractions selected in experiment (F_4,10 = 2.588, p> 0.05) <b>(b).</b></p

TLC pattern of cyanobacterial extracts.

<p>TLC patterns of methanolic extracts of lyophilized <i>Geitlerinema</i> sp. CCC728 <b>(a)</b> and <i>Arthrospira</i> sp. CCC729 <b>(c),</b> using CCl4: methanol (9:1). The most potent bands from <i>Geitlerinema</i> sp. CCC728 <b>(b)</b> and <i>Arthrospira</i> sp. CCC729 (d), were purified by a second TLC step using hexane: ethyl acetate (1:1).</p

<i>Geitlerinema</i> sp. CCC728 crude extract has no effect on some cancer biomarkers.

<p>Multiplex assay showing levels of expression of cancer biomarkers in A498 cancer cells after treatment with crude extract at 100 μg·mL^-1 in the cells showing no impact on selected biomarkers.</p

Screening for anticancer activity on cancer cell line.

<p>Calcein assay using a renal cell carcinoma (A498) cell line. Significant difference (p<0.001) between pairs, control with <i>Geitlerinema</i> CCC728 and <i>Arthrospira</i> CCC729 <b>(a),</b> Control, C2 and C3 with C4 and C5 <b>(b)</b> and Control and D1 with D3 and D4, D2 with D4 <b>(c)</b>. Control was lacking with crude extracts or TLC fractions.</p