Analysis of DNA methylation patterns under salinity stress with respect to control condition in the shoot and root of seedlings of rice varieties, IR29, Nipponbare (Nipp), Pokkali (Pokk), and Geumgangbyeo (Geum).

<p>A score of 1 and 0 represents presence and absence of bands, respectively. Values in parentheses indicate percentage of bands in each pattern which was determined by dividing number of bands in each pattern by total number of bands in all three patterns.</p

BLAST results of ten randomly selected polymorphic methylated fragments and their location on rice genome.

<p>BLAST results of ten randomly selected polymorphic methylated fragments and their location on rice genome.</p

A representative MSAP gel using the primer combination <i>Eco</i>RI-ACG/<i>Msp</i>I-AATC.

<p>Both control and salinity stressed root and shoot of rice genotypes, IR29, Nipponbare (Nipp), Pokkali (Pokk), Geumgangbyeo (Geum) were used for MSAP analysis. EH and EM refer to digestion with <i>Eco</i>RI+<i>Hpa</i>II and <i>Eco</i>RI+<i>Msp</i>I, respectively. RC: root control; RS: root stress; SC: shoot control; SS: shoot stress.</p

DNA methylation changes in shoot and root at seedling stage under non-stress and salinity stress conditions.

a<p>MSAP (%)  =  [(II+III+IV)/(I+II+III+IV)]×100;</p>b<p>Fully methylated bands (%) = [(III+IV)/(I+II+III+IV)]×100;</p>c<p>Hemimethylated bands (%) = [(II)/(I+II+III+IV)]×100.</p><p>Type I indicated absence of methylation due to the presence of bands in both <i>EcoR</i>I/<i>Hpa</i>II and <i>Eco</i>RI/<i>Msp</i>I digest; type II bands appeared only in <i>EcoR</i>I/<i>Hpa</i>II digestion but not in the <i>Eco</i>RI/<i>Msp</i>I digest; type III generated bands obtained in <i>Eco</i>RI/<i>Msp</i>I digest but not in the <i>EcoR</i>I/<i>Hpa</i>II digest; and type IV represents the absence of band in both enzyme combinations.</p