Cultivation and characteristics of the Marine Actinobacteria from the Sea water of Alang, Bhavnagar

1896-1901The methods for the sample treatment and isolation included heat and CaCO3 treatment, enrichment of the samples and use of different growth media. Since the initial microbial load was quite high, the serial dilutions were used for the isolation of the actinomycetes. Morphotypes of different colony morphology and filamentous structure were detected and selected. A distinct variation in the occurrence of the morphotypes on various media using different techniques was evident. Among the three seasons; winter samples had highest number of the morphotypes. The heat treatment of the samples significantly affected the isolation and abundance of the morphotypes. Further, highest numbers of the actinomycetes were obtained on ISP-3 (International Streptomyces Project; Oatmeal agar), ISP-6 (Peptone-yeast extract iron agar) and ISP-2 (Yeast extract-malt extract agar) media, suggesting its suitability for the growth of the marine actinomycetes. Over all, the study highlights the occurrence, cultivability and diversity of the actinomycetes of the Alang Sea water