Dottie’s Story: Teaching Complex Instruction

Analyzing the structures that frame classroom interactions and learning, and designing, implementing, and analyzing a Complex Instruction (CI) rotation were two requirements for completion of the senior seminar I taught to elementary education majors from 2002-2009. Dottie’s Story relates how the seminar was organized and how one student, Dottie, implemented and reflected upon her learning and the learning of her students. Upon reading this paper, the reader will have a good understanding of how undergraduates were taught CI. We see the various steps of Dottie’s progress through her reflective journal and note the academic and social effects of her work on several students in the kindergarten classroom in which she was a student teacher. Small sample T-tests showed significant academic gains for students in this classroom and qualitative analyses point out the value of status interventions for four students in this classroom. This paper is one chapter in an unpublished book manuscript written during my final sabbatical leave at the University of Vermont

Faster title and abstract screening? Evaluating Abstrackr, a semi-automated online screening program for systematic reviewers

BACKGROUND: Citation screening is time consuming and inefficient. We sought to evaluate the performance of Abstrackr, a semi-automated online tool for predictive title and abstract screening. METHODS: Four systematic reviews (aHUS, dietary fibre, ECHO, rituximab) were used to evaluate Abstrackr. Citations from electronic searches of biomedical databases were imported into Abstrackr, and titles and abstracts were screened and included or excluded according to the entry criteria. This process was continued until Abstrackr predicted and classified the remaining unscreened citations as relevant or irrelevant. These classification predictions were checked for accuracy against the original review decisions. Sensitivity analyses were performed to assess the effects of including case reports in the aHUS dataset whilst screening and the effects of using larger imbalanced datasets with the ECHO dataset. The performance of Abstrackr was calculated according to the number of relevant studies missed, the workload saving, the false negative rate, and the precision of the algorithm to correctly predict relevant studies for inclusion, i.e. further full text inspection. RESULTS: Of the unscreened citations, Abstrackr’s prediction algorithm correctly identified all relevant citations for the rituximab and dietary fibre reviews. However, one relevant citation in both the aHUS and ECHO reviews was incorrectly predicted as not relevant. The workload saving achieved with Abstrackr varied depending on the complexity and size of the reviews (9 % rituximab, 40 % dietary fibre, 67 % aHUS, and 57 % ECHO). The proportion of citations predicted as relevant, and therefore, warranting further full text inspection (i.e. the precision of the prediction) ranged from 16 % (aHUS) to 45 % (rituximab) and was affected by the complexity of the reviews. The false negative rate ranged from 2.4 to 21.7 %. Sensitivity analysis performed on the aHUS dataset increased the precision from 16 to 25 % and increased the workload saving by 10 % but increased the number of relevant studies missed. Sensitivity analysis performed with the larger ECHO dataset increased the workload saving (80 %) but reduced the precision (6.8 %) and increased the number of missed citations. CONCLUSIONS: Semi-automated title and abstract screening with Abstrackr has the potential to save time and reduce research waste

The process of conducting herbal medicine research through the clinical trials exemption scheme

The primary aims of this study were to develop research methodology in order to investigate the efficacy of herbal medicine in alleviating menopausal symptoms, and to prepare an application for submission to the Medicines Control Agency (MCA) to gain approval to run a randomised, double blind, placebo controlled clinical trial. A research protocol was developed which outlined the key stages of the study for subsequent review and approval by an ethics committee. An operations manual was developed to guide and instruct clinicians on how the study should be implemented. This required the development of case history notes, screening procedures, timetable flow charts and adverse event reporting cards specifically for this study. An original herbal formula was developed for this study based on a literature review of medicinal plants. A 54-page exemption from licences MLA-164 application was completed, which set out in detail the design of the study. The pharmacological properties of the medicinal plants were reviewed from the literature and listed in detail to satisfy the MCA's requirements for scientific evidence of efficacy and safety. Heavy metal analysis was performed for quality control purposes to examine the feasibility of being able to meet specification limits for metal contamination in medicinal herbs. This was conducted using Inductively Coupled Plasma-Atomic Emission Spectroscopy on a total of 16 herb samples, eight samples of Hypericum perforatum L. and eight samples of Salvia officinalis L. from cultivation sites in the UK and Europe. Mean metal ion concentrations of lead, cadmium, mercury, copper, zinc, nickel and chromium were found to be below statutory limits and guidelines, and would therefore satisfy the requirements of an MLA- 164 application for metal contamination. High Performance Liquid Chromatography was performed using UV detector and Diode-Array detector systems to determine the suitability of each method to provide a `fingerprint' analysis of the medicinal herbs for the purposes of establishing plant authenticity. The diode-array analysis was superior to simple UV detector methodology because it was able to provide greater accuracy to determine the chemical group for some unknown constituents. However, neither system provided adequate phytochemical profiles to authenticate the medicinal plants. The research study provided a novel insight into the processes required for the design and implementation of a herbal medicine clinical trial, and has expanded the current knowledge of the requirements for completing a clinical trials application (MLA-164) to the MCA

Melt-extruded polyethylene oxide (PEO) rods as drug delivery vehicles: Formulation, performance as controlled release devices and the influence of co-extruded excipients on drug release profiles

The utility of controlled release medication formulations lies in their ability to keep drugs at steady levels in the blood plasma of recipients and within the termini of the maximum and minimum effective therapeutic levels. This avoids the “ups” and “downs” of medication levels within the body which would have been the result had conventional immediate release tablets been administered instead. In the veterinary field, controlled release medications are essential¹ because of the logistical difficulties of administering drugs on a regular (e.g., daily) basis to animals. The chief advantages of controlled release veterinary medications lie in the ease with which they can be administered; decrease in stress for animals, owing to less need for rounding up and frequent dosing; and, most importantly for farmers, the reduced cost of treatment relative to that for a multiple dosage regime

Reflections on the Practicality of Good Theory

Jennifer Kennison noticed something different about the way her high school chemistry students were working together during Complex Instruction rotation. Her attention to the change in her students’ learning caused me to think about how Elizabeth Cohen’s often referenced Kurt Lewin’s comment “There is nothing so practical as a good theory.” As a result, I decided to ask two students who were teaching CI rotations if they would be interested in working together on a conference presentation that looked at their work through the eyes of Lewin’s dictum. They would take on responsibility for documenting and writing about their CI units and I, their advisor, would take on Lewin. Both Jennifer, an experienced teacher and MEd. candidate, and Bethany Brodeur, a senior elementary education major, agreed to this task. The resulting papers formed the core of our presentation at the 2004 conference of the New England Educational Research Organization. Together, they form a short volume that integrates learning about CI with the practical implications of implementation of CI at the elementary and secondary levels. This paper reports my observations of their work confirming Lewin’s dictum and Cohen’s wisdom. C

Biotransformation of explosives by the old yellow enzyme family of flavoproteins

Several independent studies of bacterial degradation of nitrate ester explosives have demonstrated the involvement of flavin-dependent oxidoreductases related to the old yellow enzyme (OYE) of yeast. Some of these enzymes also transform the nitroaromatic explosive 2,4,6-trinitrotoluene (TNT). In this work, catalytic capabilities of five members of the OYE family were compared, with a view to correlating structure and function. The activity profiles of the five enzymes differed substantially; no one compound proved to be a good substrate for all five enzymes. TNT is reduced, albeit slowly, by all five enzymes. The nature of the transformation products differed, with three of the five enzymes yielding products indicative of reduction of the aromatic ring. Our findings suggest two distinct pathways of TNT transformation, with the initial reduction of TNT being the key point of difference between the enzymes. Characterization of an active site mutant of one of the enzymes suggests a structural basis for this difference

Better duplicate detection for systematic reviewers: Evaluation of Systematic Review Assistant-Deduplication Module

BACKGROUND: A major problem arising from searching across bibliographic databases is the retrieval of duplicate citations. Removing such duplicates is an essential task to ensure systematic reviewers do not waste time screening the same citation multiple times. Although reference management software use algorithms to remove duplicate records, this is only partially successful and necessitates removing the remaining duplicates manually. This time-consuming task leads to wasted resources. We sought to evaluate the effectiveness of a newly developed deduplication program against EndNote. METHODS: A literature search of 1,988 citations was manually inspected and duplicate citations identified and coded to create a benchmark dataset. The Systematic Review Assistant-Deduplication Module (SRA-DM) was iteratively developed and tested using the benchmark dataset and compared with EndNote’s default one step auto-deduplication process matching on (‘author’, ‘year’, ‘title’). The accuracy of deduplication was reported by calculating the sensitivity and specificity. Further validation tests, with three additional benchmarked literature searches comprising a total of 4,563 citations were performed to determine the reliability of the SRA-DM algorithm. RESULTS: The sensitivity (84%) and specificity (100%) of the SRA-DM was superior to EndNote (sensitivity 51%, specificity 99.83%). Validation testing on three additional biomedical literature searches demonstrated that SRA-DM consistently achieved higher sensitivity than EndNote (90% vs 63%), (84% vs 73%) and (84% vs 64%). Furthermore, the specificity of SRA-DM was 100%, whereas the specificity of EndNote was imperfect (average 99.75%) with some unique records wrongly assigned as duplicates. Overall, there was a 42.86% increase in the number of duplicates records detected with SRA-DM compared with EndNote auto-deduplication. CONCLUSIONS: The Systematic Review Assistant-Deduplication Module offers users a reliable program to remove duplicate records with greater sensitivity and specificity than EndNote. This application will save researchers and information specialists time and avoid research waste. The deduplication program is freely available online