Optimization of production, biochemical characterization and In Vitro evaluation of the therapeutic potential of fibrinolytic enzymes from a new Bacillus Amyloliquefaciens

The capacity of fibrinolytic enzymes to degrade blood clots makes them of high relevance in medicine and in the pharmaceutical industry. In this work, forty-three microorganisms of the genus Bacillus were evaluated for their potential to produce fibrinolytic proteases. Thirty bacteria were confirmed as producers of fibrinolytic enzymes, the best results obtained for the strain Bacillus amyloliquefaciens UFPEDA 485. The optimization of the enzyme production conditions was done by a central composite design (CCD) star 23 that allowed to define the optimal conditions for soybean flour and glucose concentrations and agitation rate. The highest fibrinolytic activity (FA) of 813 U mL-1 and a degradation of blood clot in vitro of 62% were obtained in a medium with 2% (w/v) of soybean flour and 1% (w/v) glucose at 200 rpm after 48 h of cultivation, at pH 7.2 and 37 °C. The obtained fibrinolytic enzyme was characterized biochemically. Fibrinolytic activity was inhibited by PMSF (fluoride methylphenylsulfonyl - C7H7FO2S) 91.52% and EDTA (ethylenediaminetetraacetic acid - C10H16N2O8) 89.4%, confirming to be a serine- metallo protease. The optimum pH and temperature were 7.0 and 37 oC, respectively, and the enzyme was stable for 12 h. The fibrinolytic activity at physiological conditions of this enzyme produced by Bacillus amyloliquefaciens UFPEDA 485, as well as its long term stability, demonstrate that it has suitable characteristics for human and veterinary applications, and promises to be a powerful drug for the treatment of vascular diseases.We express our thanks to Coordination for the Improvement of Higher Level Education Personnel (CAPES) - Doctoral Sandwich Program (PDSE) Nº 0259/ 12-8 and National Council for Scientific and Technological Development (CNPq) - Nº 202026/2011-6 for the financial support

Traditional products and practices of indigenous people inhabiting Ramanathapuram district of Tamil Nadu

23-26Traditional knowledge plays a crucial role in establishing sustainable relationship between man and nature in the society more dependent on natural environment for their varied needs. A study was undertaken to document the indigenous products and practices related to non-farming livelihood activities of Ramanathapuram district. The data was collected with the help of focused group discussion and practitioner participatory approach. In the paper, indigenous materials with respect to non-farming livelihood activities of the people of Ramanathapuram district such as palm leaf products, traditional fishing devices and charcoal making process (Mootam) are discussed

POEMS syndrome - A case for more aggressive treatment

Annals of the Academy of Medicine Singapore366435-437AAMS

Traditional fishing practices followed by fisher folks of Tamil Nadu

543-547 India is the world's fourth largest fishing nation, accounting for over 4.39% of the global output. It is also a major contributor of foreign exchange earnings for several countries including India through export. The potential forward and backward linkages through boat building, construction of fishing harbours, fish processing etc., contribute further to diversification and strengthening of the regional and national economy. Modern fishing devices like motor boats are increasing the pollution and destroy the natural environment.&nbsp;&nbsp; But the ancient tradition of fishing by using the traditional devices like Pari, Katcha, Ootha, Sorati, Catamaran, and Karaivalai have become sustainable way of harvesting inland and marine life without damaging the environment of coral mangrove, wetland and sea-grass bed habitats. It has been practiced by the coastal fishing communities. </smarttagtype

Indigenous weather and forecast practices of Coimbatore district farmers

630-633Traditional beliefs are abundant particularly among the rural community. In many cases, traditional beliefs are existing in the form of well defined indigenous technologies which are valued much and followed in their farming system. Indigenous knowledge includes both technical and non-technical fields covering various social and religious taboos, beliefs and customs, communication patterns, music, ecology, vegetation, climate and so on. A study was conducted at Coimbatore district, Tamil Nadu of identifying the various indigenous/traditional weather forecasting methods known/practiced by the farmers. The study contained three categories of sample with a sample size of 30 each, namely Seasonal climate forecast farmers (SCF), Medium Range Weather Forecast Farmers (MRF) and Non-informed (NI) farmers. The farmers were selected using simple random sampling. The data was collected through a well structured and pre-tested interview schedule by employing personal interview technique and percentage analysis was used to analyze the data. Fifteen indigenous/traditional weather and climate related practices were identified. Among different indigenous practices, eleven practices related to rainfall, two practices related to pest and disease incidence and one each for drought and almanac has been delineated